Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Virulence Factor Profiles of Escherichia coli Isolated from Pork and Chicken Meats Obtained from Retail Markets

소매시장에서 판매하는 돼지고기와 닭고기에서 분리된 Escherichia coli의 병원성 인자

  • Choi, Sun-Keum (Department of Food and Nutrition, Chung-Ang University) ;
  • Lee, Min-Hwa (Department of Food and Nutrition, Chung-Ang University) ;
  • Lee, Bog-Hieu (Department of Food and Nutrition, Chung-Ang University) ;
  • Jung, Ji-Youn (Department of Companion and Laboratory Animals Science, Kongju National University) ;
  • Choi, Chang-Sun (Department of Food and Nutrition, Chung-Ang University)
  • 최선금 (중앙대학교 식품영양학과) ;
  • 이민화 (중앙대학교 식품영양학과) ;
  • 이복희 (중앙대학교 식품영양학과) ;
  • 정지윤 (공주대학교 특수동물학과) ;
  • 최창순 (중앙대학교 식품영양학과)
  • Published : 2010.02.28
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Abstract

The aim of this study was to investigate the virulence factor profiles of Escherichia coli strains isolated from pork and chicken meats purchased from retail markets in Korea. From 943 pork and 142 chicken meats, 217 isolates of E. coli were cultured. The presence of 11 virulence factors (elt, estI, estII, astA, stx, cdt, cnf, agg, inve, eae, afa) were detected by polymerase chain reaction. Forty one (18.9%) of 217 E. coli isolates carried at least one virulence factor. Among 175 E. coli isolates from pork, the detection rate of astA, elt, eae, estII, estI, afa, and cnf were 6.9%, 4.6%, 4.6%, 4.0%, 2.3%, 1.1%, and 0.6%, respectively. However, stx, agg, and cdt were not detected in our isolates. Therefore, we conclude that astA is the most prevalent virulence factor in E. coli isolates contaminated in pork and chicken meats in Korea.

시중에서 판매되는 계육과 돈육 가검물 1085개를 구입하고, 총 217개의 대장균 분리주를 확보하였다. 현재 알려진 대장균 병원성 인자 11개(astA, elt, estI, estII, cdt, cnf, eae, afa, stx, inve, aggR)의 보유여부를 PCR 기법으로 조사하였다. 돈육유래 대장균에서 존재하는 병원성 인자는 astA(6.9%), eae와 elt(4.6%), estII(4.0%), estI(2.3%), afa (1.1%), cnf(0.6%)의 순으로 검출되었으나, cdt, stx, aggR, inve는 검출되지 않았다. 계육에서 분리된 대장균 42개 중에서 astA와 inve를 보유하고 있는 병원성 대장균이 각각 1개씩 확인되었다. 특히 astA는 돈육과 계육에서 분리된 대장균 중에서 높은 빈도로 존재하고 있음을 확인하였으며, cnf와 afa같이 기존에 알려지지 않은 병원성 인자들의 존재도 확인되었다.

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References

  1. Bhan, M. K., Khoshoo, V., Sommerfelt, H., Raj, P., Sazawal, S., and Srivastava, R. (1989a) Enteroaggregative Escherichia coli and Salmonella associated with nondysenteric persistent diarrhea. Pediatr. Infect. Dis. J. 8, 499-502. https://doi.org/10.1097/00006454-198908000-00005
  2. Bhan, M. K., Raj, P., Levine, M.M., Kaper, J.B., Bhandari, N., Srivastava, R., Kumar, R., and Sazawal, S. (1989b) Enteroaggregative Escherichia coli associated with persistent diarrhea in a cohort of rural children in India. J. Infect.Dis. 159, 1061-1064. https://doi.org/10.1093/infdis/159.6.1061
  3. Choi, C., Kwon, D., and Chae, C. (2001) Prevalence of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 gene and its relationship with fimbrial and enterotoxin genes in E. coli isolated from diarrheic piglets. J. Vet. Diagn. Invest. 13, 26-29. https://doi.org/10.1177/104063870101300106
  4. De Rycke, J., Milon, A., and Oswald, E. (1999) Necrotoxic Escherichia coli (NTEC): Two emerging categories of human and animal pathogens. Vet. Res. 30, 221-233.
  5. Hidaka, A., Hokyo, T., Arikawa, K., Fujihara, S., Ogasawara, J., Hasa, A., Hara-Kudo, Y., and Nishikawa, Y. (2009) Multiplex real-time PCR for exhaustive detection of diarrhoeagenic Escherichia coli. J. Appl. Microbiol. 106, 410-420. https://doi.org/10.1111/j.1365-2672.2008.04043.x
  6. Ishiguro, F., Kyota, Y., Mochizuki, M., and Horikawa, T. (2005) An outbreak of diarrhea caused by Escherichia coli serogroup O169:HNM harboring a coding gene for enteroaggregative E. coli heat-stable enterotoxin 1 (astA) in Fukui Prefecture. Jpn. J. Infect. Dis. 58, 119-120.
  7. Itoh, F., Ogino, T., Itho, F., and Watanabe, H. (1992) Differentiation and detection of pathogenic determinants among diarrheogenic Escherichia coli by polymerase chain reaction using mixed primers. Nippon Rinsho. 50, 343-347.
  8. Kadhum, H. J., Ball, H. J., Oswald, E., and Rowe, M. T. (2006) Characteristics of cytotoxic necrotizing factor and cytolethal distending toxin producing Escherichia coli strains isolated from meat samples in Northern Ireland. Food Microbiol. 23, 491-497. https://doi.org/10.1016/j.fm.2005.07.003
  9. Kobayashi, K., Seto, K., Yatsuyanagi, J., Saito, S., Terao, M., Kaneko, M., Serikawa, T., Kuramoto, S., Fujisawa, T., Suzuki, R., Yamazaki, M., Hayashi, K., Matsune, W., Yasuoka, T., Horikawa, K., Murakami, K., Kawano, K., Yamada, T., and Ito, K. (2002). Presence of the genes regarding adherence factors of Escherichia coli isolates and a consideration of the procedure for detection of diarrheagenic strain. Kansenshogaku Zasshi 76, 911-920. https://doi.org/10.11150/kansenshogakuzasshi1970.76.911
  10. Le Bouguenec, Lalioui, L., du Merle, L., Jouve, M., Courcoux, P., Bouzari, S., Selvarangan, R., Nowicki, B. J., Germani, Y., Andremont, A., Gounon, P., and Garcia, M. I. (2001) Characterization of AfaE adhesins produced by extraintestinal and intestinal human Escherichia coli isolates: PCR assays for detection of Afa adhesins that do or do not recognize Dr blood group antigens. J. Clin. Microbiol.39, 1738-1745. https://doi.org/10.1128/JCM.39.5.1738-1745.2001
  11. Lee, G. Y., Jang, H. I., Hwang, I. G., and Rhee, M. S. (2009) Prevalence and classification of pathogenic Escherichia coli isolated from fresh beef, poultry, and pork in Korea. Int. J. Food Microbiol. 134, 196-200. https://doi.org/10.1016/j.ijfoodmicro.2009.06.013
  12. Levine, M. M., Prado, V., Robins-Browne, R., Lior, H., Kaper, J. B., Moseley, S. L., Gicquelais, K., Nataro, J. P., Vial, P., and Tall, B. (1988) Use of DNA probes and HEp-2 cell adherence assay to detect diarrheagenic Escherichia coli. J. Infect. Dis. 158, 224-228. https://doi.org/10.1093/infdis/158.1.224
  13. Nataro, J. P. and Kaper, J. B. (1998) Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11, 142-201.
  14. Ojeniyi, B., Ahrens, P., and Meyling, A. (1994) Detection of fimbrial and toxin genes in Escherichia coli and their prevalence in piglets with diarrhoea. The application of colony hybridization assay, polymerase chain reaction and phenotypic assays. Zentralbl. Veterinarmed. B. 41, 49-59.
  15. Osek, J., Gallien, P., Truszczynski, M., and Protz, D. (1999) The use of polymerase chain reaction for determination of virulence factors of Escherichia coli strains isolated from pigs in Poland. Comp. Immunol. Microbiol. Infect. Dis. 22, 163-174. https://doi.org/10.1016/S0147-9571(98)00083-6
  16. Savarino, S. J., Fasano, A., Watson, J., Martin, B. M., Levine, M. M., Guandalini, S., and Guerry, P. (1993) Enteroaggregative Escherichia coli heat-stable enterotoxin 1 represents another subfamily of E. coli heat-stable toxin. Proc. Natl. Acad. Sci. USA 90, 3093-3097. https://doi.org/10.1073/pnas.90.7.3093
  17. Schoonderwoerd, M., Clarke, R. C., van Dreumel, A.A., and Rawluk, S. A. (1988) Colitis in calves: natural and experimental infection with a verotoxin-producing strain of Escherichia coli O111:NM. Can. J. Vet. Res. 52, 484-487.
  18. Shuman, M. E. and Stock, A. H. (1956) Epidemiologic studies on enteropathogenic Escherichia coli diarrhea. Ann. NY Acad. Sci. 66, 108-111. https://doi.org/10.1111/j.1749-6632.1956.tb40108.x
  19. Spika, J. S., Parsons, J. E., Nordenberg, D., Wells, J. G., Gunn, R. A., and Blake, P. A. (1986) Hemolytic uremic syndrome and diarrhea associated with Escherichia coli O157: H7 in a day care center. J. Pediatr. 109, 287-291. https://doi.org/10.1016/S0022-3476(86)80386-9
  20. Subekti, D. S., Lesmana, M., Tjaniadi, P., Machpud, N., Sriwati, Sukarma, Daniel, J. C., Alexander, W. K., Campbell, J. R., Corwin, A. L., Beecham, H. J. 3rd, Simanjuntak, C., and Oyofo, B. A. (2003) Prevalence of enterotoxigenic Escherichia coli (ETEC) in hospitalized acute diarrhea patients in Denpasar, Bali, Indonesia. Diagn. Microbiol. Infect. Dis. 47, 399-405. https://doi.org/10.1016/S0732-8893(03)00120-2
  21. Toshima, H., Uenaka, E., Bi, Y., Nakamura, H., Ogasawara, J., Hase, A., Kamata, Y., and Nishikawa, Y. (2004) Detection and isolation of Escherichia coli with a coding gene for enteroaggregative Escherichia coli heat-stable enterotoxin 1 from food and comparison with fecal isolates. J. Food Prot. 67, 2117-2122.
  22. Toth, I., Hrault, F., Beutin, L., and Oswald, E. (2003) Production of cytolethal distending toxins by pathogenic Escherichia coli strains isolated from human and animal sources: establishment of the existence of a new cdt variant (Type IV). J. Clin. Microbiol. 41, 4285-4291. https://doi.org/10.1128/JCM.41.9.4285-4291.2003
  23. Vial, P. A., Robins-Browne, R., Lior, H., Prado, V., Kaper, J. B., Nataro, J. P., Maneval, D., Elsayed, A., and Levine M. M. (1988) Characterization of enteroadherent-aggregative Escherichia coli, a putative agent of diarrheal disease. J. Infect. Dis. 158, 70-79. https://doi.org/10.1093/infdis/158.1.70
  24. Wanke, C. A., Schorling, J. B., Barrett, L. J., Desouza, M. A., and Guerrant, R.L. (1991) Potential role of adherence traits of Escherichia coli in persistent diarrhea in an urban Brazilian slum. Pediatr. Infect. Dis. J. 10, 746-751. https://doi.org/10.1097/00006454-199110000-00006
  25. Yamamoto, T. and Echeverria, P. (1996) Detection of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 gene sequences in enterotoxigenic E. coli strains pathogenic for humans. Infect. Immun. 64, 1441-1445.
  26. Yamamoto, T. and Nakazawa, M. (1997) Detection and sequences of the enteroaggregative Escherichia coli heatstable enterotoxin 1 gene in enterotoxigenic E. coli strains isolated from piglets and calves with diarrhea. J. Clin. Microbiol. 35, 223-227.

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