DOI QR코드

DOI QR Code

Comparison of Measuring Methods for Somatic Cell Count in Goat Milk

측정방법에 따른 국내산 산양유의 체세포수 비교

  • Lee, Seung-Gyu (Animal Products Research and Development Division, National Institute of Animal Science, RDA) ;
  • Kim, Min-Kyung (Animal Products Research and Development Division, National Institute of Animal Science, RDA) ;
  • Lee, Yeon-Jeong (Animal Products Research and Development Division, National Institute of Animal Science, RDA) ;
  • Jeong, Seok-Geun (Animal Products Research and Development Division, National Institute of Animal Science, RDA) ;
  • Oh, Mi-Hwa (Animal Products Research and Development Division, National Institute of Animal Science, RDA) ;
  • Kim, Dong-Hun (Animal Products Research and Development Division, National Institute of Animal Science, RDA) ;
  • Park, Kwang-Wook (Sunchon National University) ;
  • Lee, Wan-Kyu (Chungbuk National University) ;
  • Ham, Jun-Sang (Animal Products Research and Development Division, National Institute of Animal Science, RDA)
  • 이승규 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 김민경 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 이연정 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 정석근 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 오미화 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 김동훈 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 박광욱 (순천대학교) ;
  • 이완규 (충북대학교) ;
  • 함준상 (농촌진흥청 국립축산과학원 축산물이용과)
  • Published : 2010.02.28

Abstract

The standard method for somatic cell counts in goat milk is the direct microscopic method after a pyronin Y-methyl stain. It has been reported, however, that are found to differ by measuring methods, but. A total of forty eight goat milk samples from eight farms were compared by pyronin Y-methyl stain, ADAM-SCC in National Institute of Animal Science, and Somacount 500 in Chungbuk Veterinary Service. The average SCC of the samples was $7.3{\times}10^5$ cells/mL by pyronin Y-methyl stain, $4.9{\times}10^5$ cells/mL by ADAM-SCC, and $11.6{\times}10^5$ cells/mL by Somacount 500. The correlations between the methods were not significant. SCC measuring equipment should be developed for reducing the SCC in goat milk, and pyronin Y-methyl green stain for estimating SCC in goat milk should be included in NVRQS Notice for livestock products processing and composition standards.

산양유의 체세포수는 측정방법에 따라 많은 차이가 보고되고 있으나 국제적인 표준방법으로 pyronin Y-methyl green stain에 의한 직접현미경법이 사용되고 있다. 8개 목장 총 48개 시료를 pyronin Y-methyl green stain에 의한 직접검경법, 국립축산과학원에서 보유중인 ADAM-SCC, 충청북도 축산위생연구소에서 보유중인 Somacount 500으로 측정하여 결과를 비교하였다. Pyronin Y-methyl green stain 측정시 전체 평균은 $7.3{\times}10^5$ cells/mL인 반면 ADAMSCC와 Somacaount 500은 각각 $4.9{\times}10^5$$11.6{\times}10^5$ cells/mL로 나타나 과소 또는 과대평가되는 결과를 보였다. 직접현미경법과 ADAM-SCC또는 Somacaount 500과의 상관계수는 각각 0.0332 또는 0.2285로 매우 낮으며 장비간의 상관계수도 0.1552로 매우 낮게 나타났다. 산양유의 체세 포수 저감을 위해서는 측정장비의 개발이 필요하며 장비를 표준화하기 위한 표준측정법으로 pyronin Y-methyl green stain의 채택이 필요하다.

Keywords

References

  1. Bently Instrumnets. Somacount 300 & 500: High volume instruments that deliver exceptional precision. Available from: http://www.bentleyinstruments.com/PDFs/Soma300500.pdf. Accessed Feb. 10. 2010.
  2. Digital Bio. ADAM-SCC. Available from: http://www.digital- bio.com/product/product01_1.php. Accessed Feb. 10. 2010.
  3. Dulin, A. M., Paape, M. J., and Wergin, W. P. (1982) Differentiation and enumeration of somatic cells in goat milk. J. Food Prot. 45, 435-439.
  4. Food and Drug Administration. Direct microscopic somatic cell count. Available from: http://www.fda.gov/downloads/ AboutFDA/ReportsManualsForms/Forms/UCM071153.pdf. Accessed Feb. 10. 2010.
  5. Haenlein, G. F. W. and Hinckley, L. S. (1995) Goat milk somatic cell count situation in USA. Int. J. Anim. Sci. 10,305-310.
  6. IDF. (1981) Laboratory methos for use in mastitis work. Bulletin No. 132. International Dairy Federation, Brussels.
  7. ISO. (1997) Milk – Enumeration of somatic cells-Part 1: Microscopic. ISO13366. International Standards Organization, Geneva.
  8. Miller, R. H., Paape, M. J., and Action, J. C. (1986) Comparison of milk somatic cell counts by Coulter and Fossomatic Counters. J. Dairy Sci. 69, 1942-1946. https://doi.org/10.3168/jds.S0022-0302(86)80621-X
  9. Packard Jr., V. S., Tatini, S., Fugua, R., Heady, J., and Gilman, C. (1992) Direct microscopic methods for bacteria or somatic cells. In: Methods for the examination of dairy products, R. T. Marshall, ed., 16th ed. Am. Public Health Assoc., Washington, DC. pp. 309-321.
  10. Shin, J. H., Jeong, S. G, Han, G. S., Jang, A., Chae, H. S., Yoo, Y. M., Ahn, J. N., Woo, K. T., Choi, S. H., Lee, W. K., and Ham, J. S. (2008) Study on the somatic cell count grade of goat milk in Korea. Korean J. Food Sci. Ani. Resour. 28, 574-579. https://doi.org/10.5851/kosfa.2008.28.5.574
  11. MIFAFF/NVRQS (2002) Raw milk sanitation grading Standards (NVRQS Notice 2002-4).
  12. MIFAFF/NVRQS (2008) Livestock products processing and composition Standards (NVRQS Notice 2008-27).

Cited by

  1. Seasonal variation of goat milk composition and somatic cell count in Jeonnam province vol.36, pp.4, 2013, https://doi.org/10.7853/kjvs.2013.36.4.263
  2. Characteristics of dairy goat milk positive reaction of the alcohol precipitation test in Korea vol.36, pp.4, 2013, https://doi.org/10.7853/kjvs.2013.36.4.255