Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Identification of 'Chunpoong' among Panax ginseng Cultivars Using Real Time PCR and SNP Marker

  • Sun, Hua (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Lee, Ok-Ran (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Yu-Jin (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Jeong, Seok-Kyu (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • In, Jun-Gyo (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kwon, Woo-Saeng (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Kim, Se-Young (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University) ;
  • Yang, Deok-Chun (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
  • Published : 2010.03.31
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Abstract

The common DNA extraction methods are indispensable for genotyping by molecular marker analysis. However, genotyping a large number of plants is painstaking. A modified 'NaOH-Tris' method used in this study reduces the extraction time while keeping the cost low and avoiding the use of hazardous chemicals. The endpoint analysis by realtime PCR tends to be fast and effective for the development of SNP markers linked to the 'Chunpoong' cultivar of Panax ginseng. The 'Chunpoong' marker was developed by a major latex-like protein gene sequence. From our results, we suggest that this method is successful in distinguishing 'Chunpoong' from a large number of ginseng cultivars.

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References

  1. Banerjce U, Izquierdo JA. Antistress and antifatigue propertiesof Panax ginseng: Comparison with piracetan. ActaPhysiol Lat Am. 32: 277-285 (1982)
  2. Sugaya A, Yuzurihara M, Tsuda T, Yasuda K, Kajiwara K,Sugaya E. Proliferative effect of ginseng saponin on neuriteextension of primary cultured neurons of the rat cerebral cortex.J Ethnopharmacol. 22: 173-181 (1998) https://doi.org/10.1016/0378-8741(88)90125-0
  3. Jo JS, Han YN, Oh HI, Park H, Sung HS, Park JI. Korean ginseng has a characteristic shape. In: Han YN, Oh HI, Park H, Sung HS, Park JI (ed), Understandiny of Korean ginseng, The Society for Korean Ginseng, Hanrimwon, Seoul, p 37- 38 (1995)
  4. Palazon J, Cusido R M, Bonfil M, Mallol A, Moyamo E,Marales C, Pinol MT. Elicitation of different Panax ginsengtransformed root phenotypes for an improvement ginsenosideproduction. Plant Physiol Biochem. 41: 1019-1025(2003) https://doi.org/10.1016/j.plaphy.2003.09.002
  5. Zheng K, Subudhi PK, Domingo J, Maopantay G, Huang N.Rapid DNA isolation for marker assisted selection in ricebreeding. Rice Genet Newslett. 12: 48 (1995)
  6. Wang H, Qi M, Cutler AJ. A simple method of preparingplant samples for PCR. Nucleic Acids Res. 21: 4153-4154(1993) https://doi.org/10.1093/nar/21.17.4153
  7. Ikeda N, Bautista NS, Yamada T, Kamijima O, Ishii, T. UltrasimpleDNA extraction method for marker-assisted selectionusing microsatellite markers in rice. Plant Mol Biol Rep. 19:27-32 (2001) https://doi.org/10.1007/BF02824075
  8. Cheung WY, Hubert N, Landry BS. A simple and rapid DNAmicroextraction method for plant, animal, and insect suitablefor RAPD and other PCR analyses. PCR Methods Appl. 3:69-70 (1993) https://doi.org/10.1101/gr.3.1.69
  9. Guidet F. A powerful new technique to quickly prepare hundredsof plant extracts for PCR and RAPD analyses. NucleicAcids Res. 22: 1772-1773 (1994) https://doi.org/10.1093/nar/22.9.1772
  10. Dilworth E, Frey JE. A rapid method for high throughputDNA extraction from plant material for PCR amplification.Plant Mol Biol Rep. 18: 61-64 (2000) https://doi.org/10.1007/BF02825295
  11. Chunwongse J, Martin GB, Tanksley SD. Pre-germinationgenotypic screening using PCR amplification of half-seeds.Theor Appl Genet. 86: 694-698 (1993)
  12. Do N, Adams RP. A simple technique for removing plantpolysaccharide contaminants from DNA. Biotechniques 10:162-6 (1991)
  13. Callaway AS, Abranches R, Scroggs J, Allen GC, ThompsonWF. High-throughput transgene copy number estimation bycompetitive PCR. Plant Mol Biol Rep. 20: 265-277 (2002) https://doi.org/10.1007/BF02782462
  14. Joshi MU, Pittman HK, Haisch CE, Verbanac KM. Real-timePCR to determine transgene copy number and to quantitatethe biolocalization of adoptively transferred cells fromEGFP-transgenic mice. Biotechniques 45: 247-258 (2008) https://doi.org/10.2144/000112913
  15. Dalla CL, Vaccari I, Mandolini M, Martinelli L. Elaborationof a Reliable Strategy Based on Real-Time PCR To CharacterizeGenetically Modified Plantlets and To Evaluate theEfficiency of a Marker Gene Removal in Grape (Vitis spp.).J Agric Food Chem. 57: 2668-2677 (2009) https://doi.org/10.1021/jf802740m
  16. Wu SB, Wirthensohn MG, Hunt P, Gibson JP, Sedgley M.High resolution melting analysis of almond SNPs derivedfrom ESTs. Theor Appl Genet. 118: 1-14 (2008) https://doi.org/10.1007/s00122-008-0870-8
  17. Mader E, Lukas B, Novak JA. Strategy to setup codominantmicrosatellite analysis for high-resolution-melting-curve-analysis(HRM). BMC Genet. 9: 69 (2008) https://doi.org/10.1186/1471-2156-9-69
  18. Garces-Claver A, Fellman SM, Gil-Ortega R, Jahn M, Arnedo-Andres MS. Identification, validation and survey of a singlenucleotide polymorphism (SNP) associated with pungencyin Capsicum spp. Theor Appl Genet. 115: 907-916 (2007) https://doi.org/10.1007/s00122-007-0617-y
  19. van Tienderen PH, de Haan AA, van der Linden CG, VosmanB. Biodiversity assessment using markers for ecologicallyimportant traits. Trends Ecol Evol. 17: 577-582 (2002) https://doi.org/10.1016/S0169-5347(02)02624-1
  20. Ohnishi Y, Tanaka T, Ozaki K, Yamada R, Suzuki H, NakamuraY. A high-throughput SNP typing system for genomewideassociation studies. J Hum Genet. 46: 471-477 (2001) https://doi.org/10.1007/s100380170047
  21. Shiokai S, Kitashiba H, Shirasawa K, Nagano K, Nishio T.Leaf-punch method to prepare a large number of PCR templatesfrom plants for SNP analysis. Mol Breeding 23:329-336 (2009) https://doi.org/10.1007/s11032-008-9244-9
  22. Garritano S, Gemignani F, Voegele C, Nguyen-Dumont T, LeCalvez-Kelm F, De Silva D, Lesueur F, Landi S, TavtigianSV. Determining the effectiveness of high resolution meltingAnalysis for SNP genotyping and mutation scanning at theTP53 locus. BMC Genet. 10: 5 (2009) https://doi.org/10.1186/1471-2156-10-5
  23. Sun H, Kim MK, Pulla RK, Kim YJ, Yang DC. Isolation andexpression analysis of a novel major latex-like protein(MLP151) gene from Panax ginseng. Mol Biol Report,Online published (2009)

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