Mycobiology

The Korean Society of Mycology (한국균학회)
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Screening of Cell Cycle-Related Genes of Pleurotus eryngii Using Yeast Mutant Strains

  • Shi, Shanliang (Department of Microbiology and Research Institute of Life Science, Gyeongsang National University) ;
  • Ro, Hyeon-Su (Department of Microbiology and Research Institute of Life Science, Gyeongsang National University)
  • Published : 2010.03.31
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Abstract

Temperature-sensitive yeast mutants were used to screen for cell cycle-related genes from Pleurotus eryngii genomic DNA. A mushroom genomic DNA library was established and each gene was screened for the ability to rescue seven Saccharomyces cerevisiae temperature-sensitive strains. Hundreds of yeast transformants were selected at restrictive temperatures over $30^{\circ}C$. Plasmids from the transformants that survived were isolated and transformed back into their host strains. The temperature sensitivity of the resulting transformants was tested from $30^{\circ}C$ to $37^{\circ}C$. Ten DNA fragments from P. eryngii were able to rescue yeast temperature-sensitive strains, and their DNA sequences were determined.

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References

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