Advances in Traditional Medicine

  • 제10권1호
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  • Pages.44-49
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  • 2010
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  • 2662-4052(pISSN)
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  • 2662-4060(eISSN)
경희대학교 융합한의과학연구소 (Kyung Hee Oriental Medicine Research Center)
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Inhibitory effect of sakuranetin on (1,3)-β-glucan synthase

  • You, Myung-Ja (Division of Nanobiochemistry, College of Natural Products, Wonkwang University) ;
  • Kim, Bo-Mi (Division of Nanobiochemistry, College of Natural Products, Wonkwang University) ;
  • Bhatt, Lok Ranjan (Division of Nanobiochemistry, College of Natural Products, Wonkwang University) ;
  • Chai, Kyu-Yun (Division of Nanobiochemistry, College of Natural Products, Wonkwang University) ;
  • Baek, Seung-Hwa (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 발행 : 2010.03.31
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초록

An examination of the kinetic properties of UDP-glucose, (1,3)-$\beta$-glucans (callose) synthase, from mung bean seedlings (Sorbus commixta cortex) shows that these enzymes have a complex relationship with UDP-glucose and various effectors. Fluorescence assay showed that deoxynojirimycin increased the inhibitory effect of (1,3)-$\beta$-glucan synthase in a concentration-dependent manner. The inhibitory effect of sakuranetin (34.34%) was higher than that of deoxynojirimycin (80.63%). Disk diffusion method revealed that sakuranetin inhibited the growth of Candida albicans to a 1.5 mm inhibition zone. These results suggest that sakuranetin, isolated from Sorbus commixta cortex extract, can be used as stable antifungal material.

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