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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Influence of the lung mechanical ventilation with injurious parameters on 7-ketocholesterol synthesis in Sus Scrofa

  • Klimenko, Oxana V. (Department of Pediatrics and Adolescent Medicine, 1st School of Medicine, Charles University) ;
  • Vobruba, Vaclav (Department of Pediatrics and Adolescent Medicine, 1st School of Medicine, Charles University) ;
  • Martasek, Pavel (Department of Pediatrics and Adolescent Medicine, 1st School of Medicine, Charles University)
  • Published : 2010.04.30
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Abstract

The aim of work was to investigate changes of 7-ketocholesterol synthesis in alveolar macrophages in the dynamic of lung mechanical ventilation with injurious parameters. The goal of in vitro part of work was to observe influence of 7-ketocholesterol on iNOS and MIP1 $\beta$ production in bronchoalveolar lavage fluid (BALF) cells. We used 17 healthy domestic pigs randomly assigned into two treatment groups: group I with mechanical ventilation with physiological parameters; group II underwent injurious ventilation with high volume tidal (VT) and low positive end expiratory pressure (PEEP). Cells were analyzed for CYP27A1 protein and gene expression levels, 7-ketocholesterol production. In alveolar macrophages of group II, we obtained increase of production of CYP27A1 protein and 7-ketocholesterol, as well as the expression of the CYP27A1 gene at the 2nd hour of ventilation. In the in vitro experiments we show dose-dependent increase of MIP1 $\beta$ and decrease of CYP27A1, iNOS protein production after 7-ketocholesterol treatment.

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References

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