Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Resistance of SOD2-transgenic petunia line to oxidative stress

  • Lee, Su-Young (Floricultural Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Han, Bong-Hee (The Foundation of Ag. Tech. Commercialization and Transfer) ;
  • Kim, Yeong-Tae (Genomics Division, National Academy of Agricultural Science, Rural Development Administration) ;
  • Kim, Jin-Seog (Chemical Biotechnology Research Center, Korea Research Institute of Chemical Technology (KRICT))
  • Received : 2010.11.16
  • Accepted : 20101131
  • Published : 2010.12.31
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Abstract

SOD2-transgenic $T_3$ petunia line (A2-36-2-1-1-35) was treated with different levels of methyl viologen (MV) to determine its resistance to oxidative stress. Four (4) levels of MV (0, 100, 200, and $400\;{\mu}M$) were applied. The SOD2-transgenic $T_3$ petunia line exhibited a very significant oxidative stress resistance at the highest MV concentration ($400\;{\mu}M$) treatment compared to non-transgenic plant. RNA and protein expression of SOD2 transgene and higher parenchyma cell density in the transgenic petunias exhibiting resistance to oxidative stress proves its contribution to the expression of its resistance to oxidative stress.

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References

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