한국어병학회지 (Journal of fish pathology)

  • 제23권2호
  • /
  • Pages.189-198
  • /
  • 2010
  • /
  • 1226-0819(pISSN)
  • /
  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

Pentachlorophenol 노출에 따른 넙치, Paralichthys olivaceus의 혈액성분의 변화

Changes of hematological parameters in olive flounder Paralichthys olivaceus exposed to pentachlorophenol

  • 강주찬 (부경대학교 수산생명의학과) ;
  • 민은영 (부경대학교 수산생명의학과)
  • Kang, Ju-Chan (Dept. of Aquatic Life Medicine, Pukyong National University) ;
  • Min, Eun-Yong (Dept. of Aquatic Life Medicine, Pukyong National University)
  • 투고 : 2010.05.03
  • 심사 : 2010.08.23
  • 발행 : 2010.08.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

PCP 노출에 따른 넙치의 혈액성상, 혈청의 유기성분, 무기성분 및 효소활성의 변화를 검토하였다. 넙치의 적혈구 수 및 Ht는 0.5 mg/kg이상의 농도에서 2주 이후, Hb는 0.5 mg/kg 이상의 농도에서 4주 이후부터 유의한 감소를 나타냈다. 넙치의 혈청 chloride 및 phosphate 농도의 변화는 관찰되지 않았지만, magnesium 농도는 0.5 mg/kg 이상의 농도에서 4주째 이후부터 유의하게 증가하였고, 혈청 calcium농도는 0.5 mg/kg 이상의 농도에서 6주째 유의한 감소가 관찰되었다. 혈청 total protein 농도는 6주째 0.5 mg/kg 이상의 농도에서 유의하게 감소하였으며, glucose 농도는 0.5 mg/kg에서 2주 후부터 유의한 증가가 관찰 되었지만, total cholesterol 농도의 유의한 차이는 관찰되지 않았다. 혈청 GOT 활성은 0.5 mg/kg의 농도에서 2주 이후, 혈청 GPT활성은 0.5 mg/kg 농도에서 4주 이후부터 유의한 증가가 관찰되었다. 또한 혈청 LDH 활성은 1.0 mg/kg의 농도에서 4주 이후, 0.5 mg/kg의 농도에서 6주에서 유의한 증가가 관찰되었으나, 혈청 AlP활성의 변화는 관찰되지 않았다.

Experiments were carried out to determine the hematological changes in olive flounder, Paralichthys olivaceus, after sub-chronic dietary pentachlorophenol (PCP) concentrations (0, 0.1, 0.5 and 1.0 mg/kg) exposure 6 weeks. The RBC count, Ht and Hb were significantly decreased in PCP 0.5 mg/kg exposed flounder compared to control group. The flounder exposed to PCP concentration 0.5 mg/kg started to increase significantly in serum magnesium concentrations after 4 weeks. The low concentration of serum calcium concentration was observed at 6 weeks of the experiment in PCP concentration 0.5 mg/kg. Although serum total protein concentrations was significantly reduced compared to the control group at PCP concentration 0.5 mg/kg, serum glucose concentration was found to be significantly increased over the control group. A significant increment of GOT, GPT and LDH activities in the flounder serum was noticed after 4 weeks at PCP concentrations over 0.5 mg/kg without typical changes of Al-P. These results indicate that flounder can be affected by PCP in terms of inorganic matter, organic matter and enzyme activity in serum when they were exposed to the PCP concentrations 0.5 mg/kg or higher.

키워드

참고문헌

  1. Bansal, S.K., Verma, S.R., Gupta, A.K. and Dalela, R.C.: Physiological dysfunction of the haemapoetic system in a fresh water teleost, Labeo rohita, following chronic chlordane exposure. Part I-Alteration in certain haematological parameters. Bull. Environ. Contam. Toxicol., 22:666-673, 1979. https://doi.org/10.1007/BF02027005
  2. Balint, T., Szegletes, T., Szegletes, Z., Halasy, K. and Nemcsok, J.: Biochemical and subcellular changes in carp exposed to the organophosphorus methidathion and the pyrethroid deltamethin. Auat. Toxicol,. 33(3-4):279-295, 1995. https://doi.org/10.1016/0166-445X(95)00029-4
  3. Borthwick, P.W. and Schimmel, S.C.: Toxicity of pentachloro-phenol and related compounds to early life stages of selected estuarine animals. In Pentachloro-phenol : chemistry, pharmacology, and environmental toxicology, pp.141-146, Rao, K.R. ed. New York, London, Plenum Press, 1978.
  4. Casillas. E. and Ames, W.: Serum chemistry of diseased English sole, Parophrys vetulus Girard, from pollured areas of Puget Sound, Washington. J. Fish Dis., 8(5):437-449, 1985. https://doi.org/10.1111/j.1365-2761.1985.tb01277.x
  5. Casillas. E. and Ames, W.: Hepatotoxic effects of CCl4 on english sole (Parophrys vetulus) : Possible indicators of liver dysfunction. Comp. Biochem. Physiol., 4C(2):397-400, 1986.
  6. Chen, X., Yin, D., Hu, S. and Hou, Y.: Immunotoxicity of pentachlorophenol on macrophage immunity and IgM secretion of the crucian carp (Carassius auratus). Bull. Environ. Contam. Toxicol., 73:153-60, 2004.
  7. Crandall, C.A. and Goodnight, C.J.: The effect of various factors on the toxicity of sodium pentachlorophenate to fish. Limnol. Oceanogr., 4:53-56, 1959. https://doi.org/10.4319/lo.1959.4.1.0053
  8. Crosby, D.G., Beynon, K.I., Greve, P.A., Korte, F., Still, G.G. and Vouk, J.W.: Environmental chemistry of pentachlorophenol. Pure Appl. Chem., 53:1051-1080, 1981. https://doi.org/10.1351/pac198153051051
  9. Davis, J.C. and Hoos, R.A.: Use of sodium pentachloro-phenate and dehydroabiotic acid as reference toxicants for salmonid bioassays. J. Fish. Res. Board Can., 32:411-416, 1975. https://doi.org/10.1139/f75-050
  10. Folmar, L.C.: Effects of chemical contaminants on blood chemistry of teleost fish : A bibliography and synopsis of selected effects. Environ. Toxicol. Chem., 12 :337-375, 1993. https://doi.org/10.1002/etc.5620120216
  11. Jee, J.H., Kim, S.G.. and Kang, J.C.: Effect of waterbrone iron on serum iron concentration and iron binding capacity of olive flounder (Paralichthys olivaceus). J. Fish. Sci. Tech., 7(1): 23-28, 2004.
  12. Joro, K. and Yasuo, I.: Effect of oral administration of cadmium on fish-I. Analytical results of the blood and bones. Bull. Jap. Soc. Fosh., 43(5):523-536, 1977. https://doi.org/10.2331/suisan.43.523
  13. Johansson, N., Larsson, A. and Lewander, K.: Metabolic effects of PCB (polychlorinated biphenyls) on the brown trout, Salmo trutta. Comp. Gen. Pharmacol., 3:310-314, 1973.
  14. Khattak, I.U.D. and Hafeez, M.A.: Effect of malathion on blood parameters of the fish, Cyprinion watsoni. Pak. J. Zool., 28(1):45-49, 1996.
  15. Ma, G., Lin, H. and Zhang, W.: Effects of cadmium on serum gonadotropin and growth hormone in common carp(Cyprinus carpio L.) J. Fish. China Shuichan Xuebao., 19(2):120-126, 1995.
  16. Mayer, K.S., Mayer, F.L. and Witt, A.: Waste transformer oil and PCB toxicity to rainbow trout. Am. Fish. Soc., 114:869-886, 1985. https://doi.org/10.1577/1548-8659(1985)114<869:WTOAPT>2.0.CO;2
  17. McDonald, D.G. and Miligan, C.L.: Chemical propeties of the blood. pp. 55-133. In fish physiology(Hoar WS, DJ Randall and AP Farrell, eds.) Academic Press, San Diego, 1992.
  18. Milligan, L.C. and Wood, C.M.: dosturbances in hematology, fluid volum distribution and circulatory function associated with low enviromental pH in the rainbow trout, Salmo gairdneri. J. Exp. Biol., 99:397-415, 1983.
  19. Rao P.P., Joseph, K.V. and Eao, K.J.: Histopathological and bio chemical changes in the liver of a fresh water fosh exposed to heptachlor. J. Nat. Conserv., 2(2):133-137, 1990.
  20. Roch, M. and Maly, E.J.: Relationship of cadmium-induced hypocalcemia with mortality in rainbow trout (Salmo gairdneri) and the influence of temperature on toxicity. J. Fish. Res. Board Can., 36(11): 1279-1303, 1979.
  21. Sakamoto, S. and Yone, Y.: Requirement of red sea bream for dietary iron. II Bull. Jap. Soc. Sci. Fish., 44(3):223-225, 1978 https://doi.org/10.2331/suisan.44.223
  22. Shen, H, Zhang, Q., Xu, R. and Wang, G.: Effects of petroleum on the sero-proteins of Tilapia mossambica. Mar. Environ. Sci., 16(1):1-5, 1997.
  23. Shich, M.S.: Changes of blood enzymes in brook trout induced by in fection with Aeromonas salonicida. J. Fish. Biol., 11:13-18, 1978.
  24. Smith, A.C. and Ramos, F.: Automated chemical analysis in fish health assessment. J. Fish. Biol., 17:445-450, 1980. https://doi.org/10.1111/j.1095-8649.1980.tb02777.x
  25. Yamazaki, K.W., Hashimoto, K., Fujii, Koyama, J., Ikeda, Y. and Ozaki, H.: Hemochemical changes in carp exposed to low cadmium concentrations. Bull. Jap. Soc. Sci. Fish., 52(3):459-466, 1986. https://doi.org/10.2331/suisan.52.459
  26. Witters, H.E., Van Puymbroeck, S., Van Den Sande, I. and Vanderborght, O.L.J.: Haematological disturvances and osmostic shifts in rainbow trout, Oncorhynchus mykiss (walbaum) under acid and aluminum exposure. J. Comp. Physiol., 160B: 563-571, 1990.
  27. Zheng, J. and Nicholson, R.A.: Influence of two naturally occurring abietane monocarboxylic acids (resin acids) and a chlorinated derivative on release of the inhibitory neurotransmitter gammaaminobutyric acid from trout brain synaptosomes. Bull. Environ. Contam. Toxicol., 56(1):114-120, 1996. https://doi.org/10.1007/s001289900017
  28. 尾崎久雄.: 魚類生理學講座 : 3. 消化生理(上), 錄書房, 東京, pp.184-188, 1971.