Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Some Characteristics of Melon necrotic spot virus-Me and Resistance Screen to the Virus in Melon Cultivars

멜론괴저반점바이러스-Me의 몇 가지 특성과 멜론 품종의 저항성 선발

  • Choi, Gug-Seoun (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Jeom-Deog (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Chung, Bong-Nam (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, In-Sook (Horticulture & Herbal Environmental Division, National Institute of Horticultural & Herbal Science, RDA) ;
  • Kwon, Soon-Bae (Gangwon Agricultural Research and Extension Services)
  • 최국선 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 조점덕 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 정봉남 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 조인숙 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 권순배 (강원도농업기술원 연구개발부)
  • Received : 2010.08.12
  • Accepted : 2010.10.04
  • Published : 2010.12.01
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Abstract

Melon necrotic spot virus (MNSV) is a very destructive disease to melon (Cucumis melo) plants. A MNSV was isolated from melon leaf showing necrotic spot symptoms at the plastic house in Naju, Korea in 2009. The isolate, designated as MNSV-Me, was identified and characterized by biological responses on several host plants, immuno captured RT-PCR and partial nucleotide sequencings of the genome. To evaluate MNSV-Me resistance in melon, thirty-five melon cultivars were mechanically inoculated on the cotyledon of the seedlings with the virus. MNSV-Me produced necrotic spots on the inoculated leaves of the all melon cultivars tested. Twenty-five cultivars were susceptible to the virus and they showed systemic necrotic spots on the leaves and/or necrosis longer than 3 cm in length on the stems within about forty days after inoculation. Five cultivars gave moderate resistance, no symptoms on the upper leaves but necrosis on the stem shorter than 3 cm in length. In an evaluation of MNSV-Me resistance in melon cultivars, 'Elstitan', 'Elsluxery', 'Betalichihage', 'Betalichi' and 'Womderfulhagae 1st' were found to have resistance by showing only faint necrosis on their stems.

Melon necrotic spot virus (MNSV)는 국내에서 재배되고 있는 멜론(Cucumis melo)에 심각한 피해를 주고 있다. 2009년 전남 나주 멜론재배단지에서 괴저반점을 나타내는 멜론 잎에서 MNSV를 분리 동정하였다. 지표식물 반응, immuno captured(IC) RT-PCR 및 염기서열 상동성 비교 등 몇몇 분석으로 이 분리주를 MNSV-Me로 명명하였다. 멜론 시판 35품종에 대한 MNSV-Me의 저항성 선발을 위하여 유묘의 자엽에 인위적 즙액 접종 방법을 이용하였다. 접종된 모든 품종의 자엽에서는 괴저반점을 형성하였다. 이들 중 25품종은 상엽으로 괴저 증상의 이행 또는 일부 개체에서는 접종 약 40일 후 줄기에 3 cm 이상의 괴저를 나타내는 감수성 반응을 나타냈다. 5품종은 상엽으로 잘 이행이 되지 않지만 줄기에 3 cm 미만의 괴저증상을 형성하는 중간 저항성을 발현하였다. 접종한 상엽으로는 바이러스 증상이 발현되지 않고, 단지 줄기에 희미한 괴저가 형성되는 얼스타이탄, 얼스럭서리, 베타리치 하계, 베타리치 및 원더플하계1호가 저항성을 나타냈다.

Keywords

References

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