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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Antifungal Activity and Biochemical Characterization of Lectin Isolated from Locular Fluid of Cherry Tomato Fruit

방울토마토 열매 locular fluid lectin의 항균성과 생화학적 특성

  • Received : 2010.02.25
  • Accepted : 2010.06.24
  • Published : 2010.06.30
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Abstract

Lectins are carbohydrate-binding and a cell-agglutinating proteins, and are concerted with a plants defence mechanism. In particular, chitin-binding lectins in locular fluid of cherry tomato fruit seemed to have a role in defending plants against fungi. The antifungal activity using lectin isolated from locular fluid of cherry tomato fruit was measured in the plant pathogen Cladosporium cucumerinum, Monosporascus cannonballus, Fusarium oxysporum, and Rhizoctonia solani. Amoung the four strains, a potent antifungal activity was detected in Cladosporium cucumerinum and Monosporascus cannonballus, not in Fusarium oxysporum, and Rhizoctonia solani. The molecular weight of this lectin isolated as double protein bands by SDS-PAGE was calculated to be 87 kDa and 47 kDa from the relative mobilities compared with those of reference molecular weight markers. The isolated lectin agglutinated human red blood cells (A, B, AB, O) treated with trypsin, and the most activity was found at B. The optimal temperature of isolated lectin was at $30^{\circ}C$. For the thermal stability, lectin was stable at $20-80^{\circ}C$. The optimal pH of this lectin was at 7.2, and showed complete loss below pH 9.0.

e-Lectin은 당 결합 단백질과 세포 응집 단백질로서, 식물방어의 역할도 하는데 방울토마토 열매의 locular fluid에는 곰팡이로부터 식물의 보호 기능을 하는 것으로 생각되는 chitin 결합 lectin이 들어 있다. 이에 본 연구에서는 방울토마토의 locular fluid로부터 최종적으로 Sephadex G-200을 통과시켜 lectin을 분리한 다음, 이의 항균성과 분자량, 적혈구 응집력, 혈액특이성, 최적 온도, 열 안정성 및 최적 pH 등의 생화학적 특성을 연구하였다. 식물성 병원균인 4개의 균주 중 Cladosporium cucumerinum과 Monosporascus cannonballus에 대해서는 항균 활성을 나타내었으나, Fusarium oxysporum과 Rhizoctonia solani에 대해서는 항균 활성을 나타내지 않았다. SDS-PAGE의 결과, 2개의 band로 나타났으며, 이의 분자량을 표준 단백질을 이용 측정하여 87 kDa와 47 kDa로 계산되었다. 사람의 A, B, O, AB형의 혈액을 사용하여 각각의 혈구응집반응을 확인한 결과, A, B, O, AB형 모두에서 응집반응이 일어났으며, 이 중 B형 혈액에서 가장 높은 활성을 나타내었다. 분리된 방울토마토 locular fluid lectin의 최적 반응 온도는 $30^{\circ}C$이며, 가장 높은 $30^{\circ}C$를 포함하는 $20-80^{\circ}C$ 범위에서 열 안정성을 보였고, 최적 pH는 7.0이다.

Keywords

References

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