Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Comparison Study on Efficacies of Disinfectants and Sanitizers Among Methods for Quantitative Surface Test

살균소독제의 정량적 표면시험방법별 유효성 비교

  • Kim, Ae-Young (Korea Health Industry Development Institute) ;
  • Kim, Yong-Su (Korea Health Industry Development Institute) ;
  • Ha, Sang-Do (Department of Food Science & Technology, Chung-Ang University)
  • Received : 2010.03.09
  • Accepted : 2010.08.12
  • Published : 2010.09.30
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Abstract

Currently, in vitro suspension tests using tubes are used as a authorized test method for sanitizers and disinfectants. However, the methods could not accurately assess the efficacy of sanitizers and disinfectant on the food-contacted surfaces in the field. This study evaluated the effectiveness of 5 kinds of representative sanitizers and disinfectants against E. coli and S. aureus to compare three quantitative surface testing methods that have been internationally standardized. As a result, the ASTM E2111-05 (ASTM(1)) test method obtained 5.18 $\pm$ 0.03 and 5.27 $\pm$ 0.04 log cfu/carrier reduction in dealing with E. coli and S. aureus, respectively, the ASTM E2197-02 (ASTM(2)) test method obtained 4.63 $\pm$ 0.04 and 3.97 $\pm$ 0.03 log cfu/carrier reduction and the CEN EN 13697 test method should 6.14 $\pm$ 0.05 and 5.31 $\pm$ 0.10 log cfu/carrier reduction in clean condition (CEN(1)) but 4.37 $\pm$ 0.02 and 4.06 $\pm$ 0.01 log cfu/carrier reduction in dirty condition (CEN(2)). Among them, CEN(1) showed the highest bactericidal effects, whereas ASTM(2) and CEN(2) revealed low performance (p < 0.05). In conclusion, the bactericidal effects of the ASTM(2) method and the CEN EN 13697 method adopting stainless steel were lower than the ASTM(1) method, which uses glass. The effectiveness assessment results among nationally accredited test methods were different each other. This implies that they could not fit for in the accurate evaluation of sanitization and disinfection on food-contact surfaces in practical food-processing fields. These results could be used as a basic data for establishment of an official surface test methods applicable in the field.

현재 살균소독제의 국가 공인시험법은 시험관 내에서 행하는 현탁액시험법으로 실제 현장의 표면 살균을 정확히 평가하기에는 무리가 있다. 본 연구는 국제적으로 표준화되어 있는 3가지 정량적 표면시험법간의 유효성 평가력 비교를 목적으로 E. coli와 S. aureus를 대상으로 시중 유통되는 대표적 5개 계열 살균소독제의 유효성을 평가하였다. 그 결과 E. coli와 S. aureus에 대한 각 방법별 살균소독력은 ASTM(1)법에서 5.18 $\pm$ 0.03, 5.27 $\pm$ 0.04 log cfu/carrier, ASTM (2)법에서 4.63 $\pm$ 0.04, 3.97 $\pm$ 0.03 log cfu/carrier, CEN EN 13697법의 청정조건(CEN(1))에서 6.14 $\pm$ 0.05, 5.31 $\pm$ 0.10 log cfu/carrier, 오염조건(CEN(2))에서 4.37 $\pm$ 0.02, 4.06 $\pm$ 0.01 log cfu/carrier로 나타났다. CEN(1)시험법이 ASTM(2)와 CEN(2) 시험법에 비해 살균소독력이 유의적으로 높게 평가되었다. 적용된 3개의 시험법 중 유리를 담체로 사용한 ASTM(1)법에 비해 스테인리스 스틸을 사용한 ASTM(2)와 CEN법 적용 시 살균소독효과가 낮게 평가되었다. 결론적으로 3 가지 국가 공인시험법간 유효성 평가결과가 달랐으며, 실제 식품제조 현장에서 사용되는 표면에서의 살균소독력을 정확히 평가하기 어렵다는 것을 확인할 수 있었다.

Keywords

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