Agronomic Variation in Anther Derived Plants of Sweet Pepper (Capsicum annuum L.) Genotypes

  • Received : 2010.05.25
  • Accepted : 2010.11.22
  • Published : 2010.12.31

Abstract

Anther derived double haploids (DHs) from sweet pepper genotypes ('Special', 'Derby', 'Bossanova', 'Fiesta', 'Debora' and 'Minipaprika') were used to study the agronomic variation in 2006. Ninety-nine successful DHs regenerants (32 from 'Special', 25 from 'Derby', 23 from 'Bossanova', 10 from 'Fiesta', 6 from 'Debora' and 3 from 'Minipaprika') were transplanted at plastic house and studied on their agronomic characters. Variation in agronomic characters was observed within the DHs of each genotype. DHs obtained from 'Derby' and 'Fiesta' exhibited wide variation in fruit yield $plant^{-1}$ whereas averaged fruit yield $plant^{-1}$ was highest in 'Derby' (1608 g) and less variation was observed in DHs of 'Bossanova'. Based on the agronomic characters expressed in DHs population at this environment, SP55, SP56, SP60, and SP116 from 'Special', SP8, SP10, SP14, SP16, and SP34 from 'Derby', SP115, SP119, SP142, SP143, SP196, and SP199 from 'Bossanova', SP41, SP45, and SP114 from 'Fiesta', SP21 from 'Debora' and SP91 from 'Minipaprika' identified as elite inbred lines and these DH lines could be used for commercial hybrids production in sweet pepper. Genetic relationship among the selected inbred lines using molecular markers and their response to diseases are further recommended to study.

Keywords

References

  1. Amaury-M, A.F. Nakazaki, T. Yamagata and T. Tanisaka, 1997. Production of double-haploid plants from Lilium longiflorum Thunb. Anther culture. Plant Sci. 123: 179-187. https://doi.org/10.1016/S0168-9452(96)04573-6
  2. Amzad H.-M., K. Konisho, M. Mihami and K. Nemoto. 2003. Somaclonal variation of regenerated plants in chili pepper (Capsicum annuum L). Euphytica, 130:233-239. https://doi.org/10.1023/A:1022856725794
  3. Brown, J.S. and E.A. Wernsman. 1982. Nature of reduced productivity of anther-derived dihaploid lines of flue-cured tobacco. Crop Sci, 22:1-5. https://doi.org/10.2135/cropsci1982.0011183X002200010001x
  4. Burk, L.G. and D.F. Matzinger, 1976. Variation among antherderived double haploids from an inbred line of tobacco. J. Hered, 67:381-384. https://doi.org/10.1093/oxfordjournals.jhered.a108757
  5. Caranta, C., A. Palloix, V. Lefebvre, and A.M. Daubeze. 1977. QTLs for components of partial resistance to cucumber mosaic virus in pepper; restriction of virus installation in host-cells. Theor. Appl. Genet, 94: 431-438.
  6. Collins, G.B. and N. Sunderland. 1974. Pollen-derived haploids of Nicotiana nightiana, N. raimondii and N. attenuata. J. Exp. Bot, 25: 1030-1039. https://doi.org/10.1093/jxb/25.6.1030
  7. Deaton, W.R., G..B. Collins, and M.T. Nielsen. 1986. Vigor and variation expressed by anther-derived doubled haploids of burley tobacco (Nicotiana tabacum L.) I. Comparison of sexual and doubled haploid populations. Euphytica, 35:33-40. https://doi.org/10.1007/BF00028538
  8. Deaton, W.R., P.D. Legg and G.B. Collins. 1982. A comparison of burley tobacco doubled haploid lines with their source-inbred cultivars. Theor. Appl. Genet, 62 : 69-74.
  9. Forster, B.P., E. Heberle-Bor, K.J. Kasha, and A.Touraev. 2007. The resurgence of haploid in higher plants. Trend in Plant Science, Vol. 12, No. 8, p. 360-1385.
  10. Greenleaf, W.H. 1986. Pepper breeding. In: Breeding vegetable crops (Bassett M.J. ed.) AVI, Westport, Connecticut, USA. pp. 67-134.
  11. Guha, S. and S.C.Maheswari. 1964. In vitro production of embryos from anthers of Datura. Nature, 204: 407.
  12. Gyulai, G., J.A. Gemesne, Z.S. Sagi, G. Venczel, P. Pinter, Z. Kristof, O. Torjek, L. Heszky, S. Botka, J. Kiss, and L. Zatyko, 2000. Double haploid development and PCR-analysis of F1 hybrid derived DH-R2 Paprika (Capsicum annuum L.) lines. Plant Physiol, 156:168-174. https://doi.org/10.1016/S0176-1617(00)80302-8
  13. Hu, K.L., S. Matsubara, and K. Murakami. 1993. Haploid plant production by anther culture in carrot (Daucus carota L.). J. Jpn. Soc. Hort. Sci, 62: 561-565. https://doi.org/10.2503/jjshs.62.561
  14. Joshi, A. and S.L. Kothari. 2007. High copper levels in the medium improve shoot bud differentiation and elongation from the cultured cotyledons of Capsicum annuum L. Plant Cell Tiss. Org. Cult, 88:127-133. https://doi.org/10.1007/s11240-006-9171-6
  15. Kintzios, S., J.-B. Drossopoulos and C.-H. Lymperopoulos. 2001. Effect of vitamins and inorganic micronutrients on callus growth and somatic embryogenesis from leaves chilli pepper. Plant Cell Tiss. Org. Cult, 67:55-62. https://doi.org/10.1023/A:1011610413177
  16. Kumashiro, T. and T. Oinuma. 1986. Genetic variation of colchicines induced and spontaneous double haploids obtained by anther culture of an inbred tobacco. Japan J. Breed. 36:355-362. https://doi.org/10.1270/jsbbs1951.36.355
  17. Larkin, P.J. and W.R.Scowcroft. 1981. Somaclonal variation-a novel source of variability from cell culture for plant improvement. Theor. Appl. Genet, 60: 197-214. https://doi.org/10.1007/BF02342540
  18. Oinuma, T. and T. Yoshida. 1974. Genetic variation among doubled haploid lines of burley tobacco varieties. Japan J. Breed, 24:211-216. https://doi.org/10.1270/jsbbs1951.24.211
  19. Orton, T.J. 1983. Somaclonal variation: theoretical and practical consideration In: Gustafson JP (ed) gene manipulation in plant improvement. Plenum Press, New York, pp. 427-468.
  20. Prakash, A.H., K. Sankara, and M. Kumar. 1997. Plant regeneration from protoplast of Capsicum annuum L. cv. California Wonder. J. Biosci, 22:339-344. https://doi.org/10.1007/BF02703236
  21. Shrestha, S.L. and W.H.Kang. 2009. Effect of genotype of donor plants on the success of anther culture in sweet pepper (Capsicum annuum L.). Kor. J. Plant Res, 22(6): 506-512.
  22. Vagera, J.N. 1990. Pepper (Capsicum ssp.) In vitro mduction of hapioids, In Bajaj, Y.P.S. (ed): Biotechnology in agriculture and forestry. Vol. 12. Haploids in crop improvement I. pp. 374-392.
  23. Valadez-Bustos, M.G., G..A. Aguado-Santacruz, G. Carrillo- Castaneda, V.H. Aguilar-Rincon, E. Espitia-Rangel, S. Montes-Hernandez, and A. Robledo-Paz. 2009. In vitro propagation and agronomic performance of regenerated chili pepper (Capsicum spp.) plants from commercially important genotypes. In Vitro Cell Dev. Biol.-Plant, 45:650-658. https://doi.org/10.1007/s11627-009-9193-y
  24. Wang, J.J., C.S. Sun, C.C. Wang, and N.F. Chein. 1973. The induction of pollen plantlets of Triticale and Capsicum annuum from anther culture. Sci. Sinica XVI, (1):147-151.