Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Isolation and Quantitative Analysis of Flavonoids with Peroxynitritescavenging Effect from the Young Leaves of Heracleum moellendorffii

어수리 어린 잎으로부터 Peroxynitrite 소거활성을 나타내는 플라보노이드 성분의 분리 및 함량분석

  • Park, Hee-Juhn (Department of Pharmaceutical Engineering, Sangji University) ;
  • Nugroho, Agung (Department of Agro-industrial Technology, Faculty of Agriculture, Lambung Mangkurat University) ;
  • Jung, Bo-Ram (Department of Pharmaceutical Engineering, Sangji University) ;
  • Won, Yu-Hwa (Department of Pharmaceutical Engineering, Sangji University) ;
  • Jung, Youn-Ju (Department of Pharmaceutical Engineering, Sangji University) ;
  • Kim, Won-Bae (Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Choi, Jae-Sue (Devision of Food Science and Biotechnology, Pukyong National University)
  • Received : 2010.04.27
  • Accepted : 2010.10.15
  • Published : 2010.10.30
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Abstract

Naturally occurring substances which strongly scavenge peroxynitrite(ONOO-) are evaluated to be beneficial for cardiovascular, diabetic, hypercholesterolemic and obese disease. To search for bioactive substances with peroxynitritescavenging activity from the young leaves of Heracleum moellendorffii(Umbelliferae) used for a mountainous vegetable, the experiment on peroxynitrite-scavenging assay, phytochemical isolation and HPLC analysis were undertaken. Activity-guided fractionation led to the separation of astragalin and hyperoside which were identified by physicochemical and spectroscopic data. The $IC_{50}$ values of astragalin and hyperoside were shown as $5.80{\pm}0.62$ and $0.560{\pm}0.26\;{\mu}M$, respectively, in the assay. The HPLC analysis led to the quantitative determination of astragalin and hyperoside by $30.0{\pm}0.01$ and $17.0{\pm}0.02\;mg/g$ dried weight, respectively.

식용으로 사용되는 산채의 일종인 어수리의 지상부로부터 peroxynitrite 소거활성을 나타내는 성분을 분리한 결과 플라보노이드 성분인 hyperoside와 astragalin이 분리되었다. 특히, 높은 활성을 보였던 hyperoside는 이 식물재료 중 함량도 높아 어수리 잎은 peroxynitrite 과잉생산시 유발될 수 있는 당뇨병, 비만, 고지혈증, 심장질환 등의 예방과 치료에 유익한 식품이라 할 수 있을 것이다.

Keywords

References

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