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Antioxidant Compounds and Antioxidant Activities of the Methanolic Extracts from Milling Fractions of Sorghum (Sorghum bicolor L. Moench)

수수 도정부위별 메탄올 추출물의 항산화성분 및 항산화활성

  • Woo, Koan-Sik (National Institute of Crop Science, Rural Development Administration) ;
  • Seo, Myung-Chul (National Institute of Crop Science, Rural Development Administration) ;
  • Kang, Jong-Rae (National Institute of Crop Science, Rural Development Administration) ;
  • Ko, Jee-Yeon (National Institute of Crop Science, Rural Development Administration) ;
  • Song, Seuk-Bo (National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Jae-Saeng (National Institute of Crop Science, Rural Development Administration) ;
  • Oh, Byeong-Geun (National Institute of Crop Science, Rural Development Administration) ;
  • Park, Gi-Do (National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Yong-Hwan (National Institute of Crop Science, Rural Development Administration) ;
  • Nam, Min-Hee (National Institute of Crop Science, Rural Development Administration) ;
  • Jeong, Heon-Sang (Dept. of Food Science and Technology, Chungbuk National University)
  • 우관식 (농촌진흥청 국립식량과학원) ;
  • 서명철 (농촌진흥청 국립식량과학원) ;
  • 강종래 (농촌진흥청 국립식량과학원) ;
  • 고지연 (농촌진흥청 국립식량과학원) ;
  • 송석보 (농촌진흥청 국립식량과학원) ;
  • 이재생 (농촌진흥청 국립식량과학원) ;
  • 오병근 (농촌진흥청 국립식량과학원) ;
  • 박기도 (농촌진흥청 국립식량과학원) ;
  • 이용환 (농촌진흥청 국립식량과학원) ;
  • 남민희 (농촌진흥청 국립식량과학원) ;
  • 정헌상 (충북대학교 식품공학과)
  • Received : 2010.07.26
  • Accepted : 2010.09.07
  • Published : 2010.11.30

Abstract

The purpose of this study was to evaluate the antioxidant compounds and their activities of the methanolic extracts from milling fractions of sorghum. To determine the antioxidant compounds in the methanolic extract from the milling fractions, the content of polyphenol, flavonoids, tannin, anthocyanins and proanthocyanidin were measured by spectrophotometric methods. These were evaluated for antioxidative activities by ABTS and DPPH radical scavenging assays. The extraction yield of hull, bran and grain of sorghum were 9.95, 19.05 and 2.94%, respectively. The methanolic extracts from sorghum bran showed generally higher antioxidant activities than the extracts from hull and grain of sorghum. In addition, antioxidant compounds distributed much higher contents in sorghum bran extract than in the extracts from hull and grain of sorghum. A significant correlation was also noted between free radical scavenging activity and polyphenolic compounds. The results of this study show that notable antioxidant activity in sorghum bran is considered to have significant health benefits.

본 연구에서는 황금찰수수의 도정분획별 메탄올 추출물의 항산화성분과 항산화활성을 비교 분석하고자 하였다. 항산화성분으로 총 polyphenol, flavonoid, tannin, anthocyanin 및 proanthocyanidin 함량을 측정하였으며, 항산화활성은 ABTS와 DPPH radical 소거활성을 측정하였다. 추출수율은 각각 수수의 hull, bran 및 grain으로 구분하여 80% 메탄올로 추출하여 용매를 완전히 제거한 후 수율을 측정한 결과 9.95, 19.05 및 2.94%로 나타났다. 메탄올 추출물의 추출수율은 hull, bran 및 grain층에서 각각 9.95, 19.05 및 2.94%의 수율을 보였다. 실험결과 bran의 추출물이 hull이나 grain 추출물보다 높은 함량의 항산화성분을 함유하고 있는 것을 확인할 수 있었다. 또한 이러한 항산화성분이 다량 분포하는 bran 추출물이 ABTS와 DPPH radical 소거활성이 각각 66.37 및 36.56 mg TE/g sample으로 나타나 hull이나 grain 추출물보다 우수한 항산화활성을 가지는 것으로 나타났다. 본 연구결과 수수의 bran은 기능성식품 제조에 있어 좋은 소재로 활용가능성이 높을 것으로 예상된다.

Keywords

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