Korean Journal of Breeding Science (한국육종학회지)

The Korean Society of Breeding Science (한국육종학회)
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Chloroplast-targeted Expression of PsAPX1 Enhances Tolerance to Various Environmental Stresses in Transgenic Rice

엽록체에 과발현된 PsAPX1 유전자 형질전환 벼의 다양한 환경스트레스 내성 증진

  • 박향미 (농촌진흥청 국립식량과학원) ;
  • 김율호 (농촌진흥청 국립식량과학원) ;
  • 최만수 (농촌진흥청 국립식량과학원) ;
  • 이재은 (농촌진흥청 국립식량과학원) ;
  • 최인배 (농촌진흥청 국립식량과학원) ;
  • 최임수 (농촌진흥청 국립식량과학원) ;
  • 신동범 (농촌진흥청 국립식량과학원) ;
  • 이장용 (농촌진흥청 국립농업과학원) ;
  • 곽상수 (한국생명공학연구원) ;
  • 권석윤 (한국생명공학연구원)
  • Received : 2009.09.06
  • Published : 20090900
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Abstract

Oxidative stress is a major damaging factor for plants exposed to environmental stresses. In order to develop transgenic rice plants with enhanced tolerance to various environmental stresses, PsAPX1, the gene of ascorbate peroxidase isolated from Pisum sativum was expressed in chloroplast under the control of an oxidative stress inducible sweet potato peroxidase2 (SWPA2) promoter (referred to as PsAPX1 plants). PsAPX1 transgenic plants showed enhanced tolerance to various environmental stresses, such as 170 mM NaCl, UV-B, ozone, 20% PEG, and drought in compared with non-transgenic (NT) plants. These results suggest that chloroplast-targeted over-expression of PsAPX1 gene could be very useful strategy for developing transgenic rice plants with increased tolerance to environmental stresses.

1. 본 연구에서는 완두유래의 세포질성 PsAPX1 유전자를 대상으로 산화스트레스 유도성 프로모터를 연결하여 엽록체에 targeting 되는 과발현 운반체를 제작하고 벼에 도입한 결과 형질전환체에서 도입유전자 수가 1~3 copy인 것으로 나타나, 적은 수의 유전자가 안정적으로 도입되었음을 확인하였다. 2. 염, 오존, 자외선, 한발과 같은 다양한 환경스트레스 조건에서 내성이 증진된 우수 계통을 선발하기 위하여 작성된 형질전환 벼 계통들을 대상으로 생물검정을 실시한 결과, 독립계통별로 스트레스원에 대한 반응에 차이가 있음을 확인하였으며, 오존이나 자외선과 같은 산화스트레스 조건에서 내성이 증진된 우수 계통들을 선발하였다. 3. 이러한 결과를 바탕으로, 다양한 환경스트레스에 내성이 증진된 우수한 형질전환 벼 계통들을 선발하기 위하여 최소한의 독립라인을 작성하여야 함을 확인하였으며, 선발된 계통들은 새로운 육종 소재로 활용될 수 있을 것으로 기대된다.

Keywords

Acknowledgement

Supported by : 농촌진흥청

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