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Chloroplast-targeted Expression of PsAPX1 Enhances Tolerance to Various Environmental Stresses in Transgenic Rice  

Park, Hyang-Mi (National Institute of Crop Science, RDA)
Kim, Yul-Ho (National Institute of Crop Science, RDA)
Choi, Man-Soo (National Institute of Crop Science, RDA)
Lee, Jae-Eun (National Institute of Crop Science, RDA)
Choi, In-Bea (National Institute of Crop Science, RDA)
Choi, Im-Soo (National Institute of Crop Science, RDA)
Shin, Dong-Bum (National Institute of Crop Science, RDA)
Lee, Jang-Yong (National Academy of Agricultural Science, RDA)
Kwak, Sang-Soo (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Kwon, Suk-Yoon (Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Korean Journal of Breeding Science / v.41, no.3, 2009 , pp. 261-270 More about this Journal
Abstract
Oxidative stress is a major damaging factor for plants exposed to environmental stresses. In order to develop transgenic rice plants with enhanced tolerance to various environmental stresses, PsAPX1, the gene of ascorbate peroxidase isolated from Pisum sativum was expressed in chloroplast under the control of an oxidative stress inducible sweet potato peroxidase2 (SWPA2) promoter (referred to as PsAPX1 plants). PsAPX1 transgenic plants showed enhanced tolerance to various environmental stresses, such as 170 mM NaCl, UV-B, ozone, 20% PEG, and drought in compared with non-transgenic (NT) plants. These results suggest that chloroplast-targeted over-expression of PsAPX1 gene could be very useful strategy for developing transgenic rice plants with increased tolerance to environmental stresses.
Keywords
Transgenic rice; PsAPX1; Environmental stress; Chloroplast-targeting; Antioxidant enzyme; Ascorbate peroxidase;
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Times Cited By KSCI : 3  (Citation Analysis)
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