Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
권호 표지

Distribution and Biodiversity of Lactic Acid Bacteria Having Bacteriocin-like Activity from Fresh Fruits and Vegetables

신선 과채류에 존재하는 박테리오신 유사 활성을 지니는 유산균의 분포와 다양성

  • Park, Young-Seo (Department of Food Science and Biotechnology, Kyungwon University) ;
  • Jang, Jae Kweon (Department of Food Science, Chungkang College of Cultural Industries) ;
  • Choi, Young Jin (Department of Agricultural Biotechnology, Seoul National University) ;
  • Chung, Myong-Soo (Department of Food Science and Engineering, Ewha Womans University) ;
  • Park, Hoon (Department of Food Science, Sunmoon University) ;
  • Shim, Kun-Sub (Greenbio Co., Ltd.)
  • 박영서 (경원대학교 식품생물공학과) ;
  • 장재권 (청강문화산업대학 식품과학과) ;
  • 최영진 (서울대학교 식품생명공학전공) ;
  • 정명수 (이화여자대학교 식품공학과) ;
  • 박훈 (선문대학교 식품과학과) ;
  • 심건섭 ((주)그린바이오)
  • Received : 2008.12.08
  • Accepted : 2009.01.15
  • Published : 2009.02.28
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Abstract

From the 25 fresh fruits and vegetable products, 1,250 isolates grown on MRS agar media were screened for the inhibitory activity on the growth of Escherichia coli 0157:H7, Listeria monocytogenes, and Bacillus cereus as well as Lactobacillus plantarum, L. casei, and Lactococcus lactis subsp. lactis. Among them, 607 isolates (49% of total isolates) from 23 different foods produced growth inhibitory activity on the E. coli 0157:H7, L. monocytogenes, or B. cereus. When these isolates were screened for the inhibitory activity on the growth of L. plantarum, L. casei, and Lactococcus lactis subsp., 24 isolates (3% of total isolates) from 7 food samples showed bacteriocin-like activity. These isolates had typical physiological characteristics of lactic acid bacteria, which indicated these isolates were strains of lactic acid bacteria. The inhibitor from 3 out of 24 revealed as nicin. From the RAPD-PCR profiles, 24 strains was classified and it was also indicated that most of the strains isolated from same produce showed similar phylogenetic profile.

25 종류의 신선 과채류로부터 MRS 한천 고체배지에서 생육하는 1,250주의 유산균을 분리한 후 식중독 세균인 Escherichia coli 0157:H7, Listeria monocytogenes, Bacillus cereus와 유산균인 Lactobacillus plantarum, L. casei, Lactococcus lactis subsp. lactis에 대한 생육저해활성을 조사하였다. 조사한 1,250 균주 중에서 23 종류의 과채류에 존재하는 607 균주(총 조사균주의 49%)가 E. coli 0157:H7, L. monocytogenes 또는 B. cereus에 대한 항균활성을 나타내었다. 이 균주들의 L. plantarum, L. casei, Lactococcus lactis subsp. lactis에 대한 항균활성을 측정한 결과, 7 종류의 과채류에 존재하는 24 균주(총 균주의 3%)가 박테리오신 유사활성을 나타내었으며, 이 균주들의 생리적 형태학적 특징을 조사한 결과 전형적인 유산균의 특징을 지니고 있었다. 24 균주 중에서 3 균주는 nisin을 생산하는 것으로 판단되었으며, RAPD-PCR 분석을 통하여 동일한 과채류에 존재하는 균주들은 유사한 분류학적 특징을 지니고 있는 것으로 확인되었다.

Keywords

Acknowledgement

Supported by : 농림부

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