Food Science and Biotechnology

  • 제18권5호
  • /
  • Pages.1063-1070
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  • 2009
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  • 1226-7708(pISSN)
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  • 2092-6456(eISSN)
한국식품과학회 (Korean Society of Food Science and Technology)
권호 표지

Anti-inflammatory Effect of Dangyuja (Citrus grandis Osbeck) Leaves in LPS-stimulated RAW 264.7 Cells

  • Yang, Eun-Jin (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Lee, Hye-Ja (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Kang, Gyeoung-Jin (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Park, Sun-Soon (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Yoon, Weon-Jong (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Kang, Hee-Kyoung (Department of Pharmacology, College of Medicine, Jeju National University) ;
  • Cho, So-Mi Kim (Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University) ;
  • Yoo, Eun-Sook (Department of Pharmacology, College of Medicine, Jeju National University)
  • 발행 : 2009.10.31
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초록

Dangyuja (Citrus grandis Osbeck) is a native plant growing only on Jeju Island in Korea. In this study, antiinflammatory effect of dangyuja leaves on a murine macrophage cell line was investigated. RAW 264.7 murine macrophage cells were stimulated with lipopolysaccharide (LPS, $1{\mu}g/mL$) to induce expression of pro-inflammatory markers [interleukin (IL)-6 and inducible nitric oxide synthase (iNOS)]. The crude extract (80% MeOH Ex.) and solvent fractions (hexane, $CHCl_3$, EtOAc, BuOH, and $H_2O$ Ex.) were obtained from dangyuja leaves. The $CHCl_3$ fraction inhibited the nitric oxide (NO) and IL-6 production in a dose-dependent manner. Also, the $CHCl_3$ fraction inhibited mRNA expression and protein levels of iNOS in a dose-dependent manner. Furthermore, the $CHCl_3$ fraction inhibited LPS-induced nuclear factor (NF)-${\kappa}B$ activation and phosphorylation of mitogen-activated protein kinases (MAPKs: ERK, JNK, and p38). These results suggest that dangyuja leaves may inhibit LPS-induced production of inflammatory markers by blocking NF-${\kappa}B$ and MAPKs signaling in RAW 264.7 cells.

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