Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of Lactosucrose from Sucrose and Lactose by a Levansucrase from Zymomonas mobilis

  • Han, Woo-Cheul (Department of Food and Nutrition, Kangwon National University) ;
  • Byun, Sun-Ho (Department of Food and Nutrition, Kangwon National University) ;
  • Kim, Mi-Hyun (Department of Food and Nutrition, Kangwon National University) ;
  • Sohn, Eun-Hwa (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Lim, Jung-Dae (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Um, Byung-Hun (Korean Institute of Science and Technology Gangneung Institute) ;
  • Kim, Chul-Ho (Biotechnology Research Division, Jeonbuk Branch Institute Molecular Bioprocess Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kang, Soon-Ah (Department of Fermented Food Science, Seoul University of Venture and Information) ;
  • Jang, Ki-Hyo (Department of Food and Nutrition, Kangwon National University)
  • Published : 2009.10.31
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Abstract

Lactosucrose ($4^G-\beta$-D-galactosylsucrose) is an oligosaccharide consisting of galactose, glucose, and fructose. In this study, we prepared lactosucrose from lactose and sucrose using a levansucrase derived from Zymomonas mobilis. Optimum conditions for lactosucrose formation were $23^{\circ}C$, pH 7.0, 18.0% (w/v) lactose monohydrate, and 18% (w/v) sucrose as substrates, and 1 unit of enzyme/ml of reaction mixture. Under these conditions, the lactosucrose conversion efficiency was 28.5%. The product was purified and confirmed to be O-$\beta$-D-galactopyranosyl-($1{\rightarrow}4$)-O-$\beta$)-D-glucopyranosyl-($1{\rightarrow}2$)-$\beta$-D-fructofuranoside, or lactosucrose. A mixed-enzyme system containing a levansucrase and a glucose oxidase was applied in order to increase the efficiency of lactose and sucrose conversion to lactosucrose, which rose to 43.2% as a result.

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References

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