Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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A Novel pH-Stable, Bifunctional Xylanase Isolated from a Deep-Sea Microorganism, Demequina sp. JK4

  • Meng, Xin (State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University) ;
  • Shao, Zongze (Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State of Oceanic Administration) ;
  • Hong, Yuzhi (College of Plant Science and Technology, Huazhong Agricultural University) ;
  • Lin, Ling (State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University) ;
  • Li, Chanjuan (State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University) ;
  • Liu, Ziduo (State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University)
  • Published : 2009.10.31
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Abstract

A genomic library was constructed to clone a xylanase gene (Mxyn10) from Demequina sp. JK4 isolated from a deep sea. Mxyn10 encoded a 471 residue protein with a calculated molecular mass of 49 kDa. This protein showed the highest sequence identity (70%) with the xylanase from Streptomyces lividans. Mxyn10 contains a catalytic domain that belongs to the glycoside hydrolase family 10 (GH10) and a carbohydrate-binding module (CBM) belonging to family 2. The optimum pH and temperature for enzymatic activity were pH 5.5 and $55^{\circ}C$, respectively. Mxyn10 exhibited good pH stability, remaining stable after treatment with buffers ranging from pH 3.5 to 10.0. The protein was not significantly affected by a variety of chemical reagents, including some compounds that usually inhibit the activity of other related enzymes. In addition, Mxyn10 showed activity on cellulose. These properties mark Mxyn10 as a potential enzyme for industrial application and saccharification processes essential for bioethanol production.

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References

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