KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
권호 표지

A Genome-wide Approach for Functional Analysis Using Rice Mutant

  • Yim, Won-Cheol (Department of Plant Biotechnology, Dongguk University) ;
  • Kim, Dong-Sub (Radiation Research Center for Bio-technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Moon, Jun-Cheol (Institute of Life Science & Natural Resources, Korea University) ;
  • Jang, Cheol-Seong (Plant Genomics Lab., Division of Bio-resources Technology, Kangwon National University) ;
  • Lee, Byung-Moo (Department of Plant Biotechnology, Dongguk University)
  • Published : 2009.09.30
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Abstract

Rapid extension of genomic database leads to the remarkable advance of functional genomics. This study proposes a novel methodology of functional analysis using 5-methyltrytophan (5 MT) mutant together with their 2-DE analysis and public microarray database. A total of 24 proteins was changed in 5 MT mutant and four remarkably different expressed proteins were identified. Among them, three spots were converted to Affymetrix probe. A total of 155 microarray samples from Gene Expression Omnibus (GEO) in NCBI was retrieved and followed by constructing gene co-expression networks over a broad range of biological issues through Self-Organising Tree Algorithm. Three co-expressing gene clusters were retrieved and each functional categorization with differential expression pattern was exhibited from 5 MT resistance mutant rice. It was indicated new co-expression networks in the mutant. This study suggests that on investigating possibility which correspond 2-DE to microarray database with their full potential.

Keywords

References

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