Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Antimicrobial Effect of Edible Pullulan Film Containing Natural Antimicrobial Material on Cariogenic Bacteria

천연물 유래 항균물질을 포함한 가식성 Pullulan 필름의 충치유발균에 대한 항균효과

  • Kim, Ki-Myong (Jeonnam Biofood Technology Center) ;
  • Hwang, Kwon-Tack (Dept. of Food & Nutrition, Nambu University) ;
  • You, Sang-Guan (Dept. of Marine Food Science and Technology, Gangneung-Wonju National University) ;
  • Lee, Ung-Soo (Division of Food and Biotechnology, Chungju National University) ;
  • Jung, Kyung-Hwan (Division of Food and Biotechnology, Chungju National University) ;
  • Moon, Sung-Kwon (Division of Food and Biotechnology, Chungju National University) ;
  • Choi, Won-Seok (Division of Food and Biotechnology, Chungju National University)
  • 김기명 (전라남도 식품산업연구센터) ;
  • 황권택 (남부대학교 식품영양학과) ;
  • 유상권 (강릉원주대학교 해양생명공학부) ;
  • 이웅수 (충주대학교 식품생명공학부) ;
  • 정경환 (충주대학교 식품생명공학부) ;
  • 문성권 (충주대학교 식품생명공학부) ;
  • 최원석 (충주대학교 식품생명공학부)
  • Published : 2009.10.31
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Abstract

This study was focused on the development of anticariogenic edible films using pullulan containing grapefruit seed extract (GFSE), polylysine or propolis. According to the result of antimicrobial activity (disc diffusion method) of GFSE, polylysine and propolis against Streptococcus mutans, antimicrobial pullulan film was produced by adding grapefruit seed extract. The optimum combination of pullulan and sorbitol (plasticizer) was 10$\sim$15% (w/v) and 40$\sim$50% of pullulan (w/w), respectively. Minimum concentration of grapefruit seed extract for growth inhibition of Str. mutans was 50 ppm in medium. Formulation of antimicrobial pullulan films containing grape seed extract was established and these results evidently showed potential for commercial application.

천연항균물질인 자몽종자추출물(grapefruit seed extract), 폴리라이신(polylysine) 및 프로폴리스(propolis)의 충치유발균 Streptococcus mutans에 대한 항균활성을 측정하여 가식성필름에 첨가 천연고분자 필름을 제조함으로써, 충치예방 및 구강청결 등의 치의적 상품개발 가능성을 타진해 보고자 하였다. Disc method를 이용한 예비 항균활성실험 결과, 자몽종자추출액의 경우 10%(w/w)의 농도에서 Streptococcus mutans 균에 대해 확실한 항균활성이 나타나, 이를 pullulan 필름제조 용액에 첨가하여 항균성 가식필름을 제조하였다. Pullulan 10$\sim$15%(w/w)의 농도와 사용된 pullulan 양의 40$\sim$50%(w/w)의 sorbitol 농도에서 적절한 항균필름이 제조되었다. 자몽종자추출액 50 ppm을 함유한 pullulan 필름은 Streptococcus mutans의 활성을 억제하는 것으로 나타나, 자몽종자추출액을 함유한 가식성 항균필름은 충치예방상품으로 개발 가능성이 높을 것으로 사료된다.

Keywords

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