KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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On-line monitoring of microorganism cultivation processes using optical sensing membranes for simultaneous detection of dissolved oxygen and pH

용존산소와 pH의 동시 검출용 광학 센서 막을 이용한 미생물 발효공정의 온라인 모니터링

  • 김춘광 (전남대학교 물질.생물화학공학과) ;
  • 이종일 (전남대학교 응용화학공학부)
  • Published : 2009.02.28
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Abstract

An optical sensing membrane has been fabricated to measure the concentration of dissolved oxygen(DO) and pH value simultaneously. It has employed HPTS as a pH sensitive dye and a ruthenium(II) complex as a DO sensitive dye. The sensing membrane has been applied to wells in a 24-well microtiter plate. Using the 24-well microtiter plate the concentrations of dissolved oxygen and pH values have been on-line monitored during the cultivations of E.coli DH5${\alpha}$, B.cereus 318 and P.pastoris X-33. On-line monitoring of DO and pH in microorganism cultivation processes showed good performance of the sensing membrane containing 5 mM HPTS and 2 or 5 mg/mL Rudpp.

Keywords

References

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