Development of a Microbial Biosurfactant Production Process

미생물에 의한 생물 계면활성제 대량생산을 위한 기술개발

  • Kim, Ju-Hyun (Department of Biotechnology, Seokyeong University) ;
  • Kim, Gi-Eun (Department of Biotechnology, Seokyeong University)
  • Published : 2009.04.29

Abstract

The microbial biosurfactants can be substituted to the chemical detergents in some industrial processes. In this study we developed a biotechnological processes for the biosurfactants with microorganisms. The biosurfactants have a lot of advantages in comparision with the chemical surfactants. They are proenvironmental even during and after industrial use. But there are not so many kinds of biosurfactants. The production cost and the end price is much higher than the chemical surfactants. But nowdays there are many kinds of microorganisms, which can produce the surfactants in large quantity and fast. We tried to develop a production process for the large scale with some microorganisms. At first Candida bombicola KCTC 7145, Sphingomonas chungbukensis KCTC 2955 and Sphingomonas yanoikuyae KCTC 2818 are cultivated and studied. For the large scale production process we used molasses as a complex medium and tried to optimize the process. Molasses contains 17 to 25% of water, 45 to 50% of sugar and 25% of carbohydrate, it can be fully used as a substrate. The microorganisms have been cultivated in the diluted media with molasses 2, 5, 8 and 10%, respectively, The optimal conditions for the cultivation and the production process have been studied. For the study the optical density, glucose concentration and the surface tension were measured. Candida bombicola KCTC 7145 and the 5% molasses media was selected as an optimal condition for the production process of a biosurfactant. During cultivation of Candida bombicola KCTC 7145 in the 5% molasses medium kerosene and corn oil were added for promoting the biosurfactants.

Keywords

References

  1. Lim, K. H. (1996), Their structure, properties, and applications, J. Korean Oil Chemist's Soc. 13, 1-20
  2. Van Dyke, M. I., P. Couture, M. Brauer, H. Lee, and J. T. Trevors (1993), Pseudomonas aeruginosa UG2 rhanmolipid biosurfactants : structural chracterization and their use in removing hydrophobic compounds from soil. Can J. Microbial. 39(11), 1071-1078 https://doi.org/10.1139/m93-162
  3. Kosaric, N., C. C. Gray, and W. L. Carin (1983), Microbial emulsifiers and deemulsifiers. Biotechnology 3, 575-592
  4. Layman, P. (1985), Industrial set for strong growth. Chem. Eng. News 63, 23
  5. Robert, M., M. E. Mercade, M. P. Bosch, J. L. Parra, M. J. Espuny, M. A. Manresa, and J. Guinea (1989), Effect of the carbon source on biosurfactant production by Pseudomonas aeruginosa 44T. Biotechnol, Lett. 11, 871-874 https://doi.org/10.1007/BF01026843
  6. Lang, S. (2002), Biological amphiphiles (microbial biosurfactants). Current Opinion in colloid & Interface Science. 7, 12-20 https://doi.org/10.1016/S1359-0294(02)00007-9
  7. Desai, J. D. and I. M. Banet (1997), Microbial production of surfactants and their commercial potentiaI, Microbiol. Mol. Biol. Rev. 61(1), 47-64
  8. Banat, I. M., R. S. Markkar, and S. S. Cometra (2000), Potential commercial appIications of microbial surfactants, Appl. Micribial. Biotechnol. 53, 495-508 https://doi.org/10.1007/s002530051648
  9. Milton, M. J. (1987), Surfactants in engineering technology, Surfactant Science Series 26, Marcell Dekker, New York
  10. Dunvnjak, J. D., G. Cooper, and N. Kosaric (1982), Production of biosurfactant by Arthrobacter paraffineus ATCC 19558, Biotechnol. Bioeng. 24, 165-175 https://doi.org/10.1002/bit.260240114
  11. Poremba, K., W. Gunkel, S. Lang, and F. Wagner (1991), Marine biosurfactants, III. toxicity testing with marine microorganism and comparison with synthetic surfactants, Z. Naturforch 46c, 21-216, 14
  12. Cooper, D. G. and D. A. Paddock (1984), ' Production of a biosurfactant from Torulopsis bombicola', Appl. Environ. Microbiol. 47, 173-176
  13. Zajic, J. E., H. Gignard, and D. F. Gerson (1977), Properties and biodegradation of a bioemulsifier from Corynebacterium hydrocarboclastus, Biotechnol. Bioeng. 19, 1303-1320 https://doi.org/10.1002/bit.260190905
  14. Fiechter, A. (1992), Integrated systems for biosurfactant synthesis. Pure Appl. Chem. 64(11), 1739-1743 https://doi.org/10.1351/pac199264111739
  15. Beersteacher, E. (1954), Petroleum Microbiology. Elsevier. Houston
  16. Bognolo, G. (1999), Biosurfactants as emulsifying agents for hydrocarbons. Colloids surfaces A: Physicochem Eng Aspects 152, 41-52 https://doi.org/10.1016/S0927-7757(98)00684-0
  17. Rosen. M. J. (1989), in Surfactant and Interfacial Phenomena, 2nd ed., Wiley. New york
  18. MacFaddin. J. F. (1984), Biochemical Tests for Identification for Medical Bacterial, 2nd ed. Williams and Wilkins Co., Baltimore, USA
  19. Schippers, C., K. Gessner, T. Muller, and T. Scheper (2000), Microbial degradation of phenanthrene by addition of a sophorelipid mixture. J. Biotechnol. 83(3), 189-198 https://doi.org/10.1016/S0168-1656(00)00304-7
  20. Um, H. J., M. H. Kang, Y. P. Kim, S. J. Kim, and Y. C. Kim (2003), Sequence and phylogenetic analysis of the phnS gene encoding 2-hydroxy chromene-2- carboxylate isomerase in Sphingomonas chungbukensis DJ77. The Korean Journal of Microbiology 39(3), 123-127
  21. Makkar, R. S. and S. C. Cameotra, (1997b), Utilization of molasses for biosurfactant production by two Bacillus strains at thermophilic conditions. J. Am. Oil. Chem. Soc. 74(7), 887-889 https://doi.org/10.1007/s11746-997-0233-7
  22. Magaritis, A., A. Kennedy, J. E. Zajic, and D. F. Gerson (1979), Biosurfactant production by Nocardia erythropolis. Dev. lnd. Microbiol. 20, 623-630
  23. Massaki, M., D. Hiromi, T. Toshifumi, M. Satoru, S. Yasutsugu, and I. Tadayuki (1993), A new lipopeptide biosurfactant produced by Arthrobacter sp. Strain MIS38. J. Bacteriol. 175, 6459-6466 https://doi.org/10.1128/jb.175.20.6459-6466.1993
  24. Najafpour, G. D. and C. P. Shan (2003), Enzymatic hydrolysis of molasses. Bioresour. Technol. 86, 91-94 https://doi.org/10.1016/S0960-8524(02)00103-7
  25. Shuler, M. L., F. Karg (2003), Bioprocess engineering, Prentice Hall. 194-196
  26. Kim, S. H., E. J. Lim, K. S. Choi, Y. K. Jeong, K. L. Jang, and T. H. Lee (1996), Emulsifying agent production by Acinetobacter sp. BE-254, Kor. J. Appl. Microbial. Biotechnol. 24, 206-212
  27. Asmer, H., S. Lang., F. Wagner, and V. Wray (1988), Microbial production, structureelucidation and bioconversion of sophorose lipids. J. Am Oil Chem Soc. 65, 1460-1466 https://doi.org/10.1007/BF02898308
  28. Ito, A., S. Inoue (1982), Sopholipids from Toulopsis bombicola; possible relation to alkane uptake. Appl Environ Microbial. 43, 1278-1283
  29. Guεrra-Santos , L. H., O. Kappeli, and A. Flechter (1986), Dependence of Pseudomonas aeruginosa continuous culture biosurfactant production on nutritional and environmental factors. Appl. Microbiol. Biotechnol. 24, 443-448 https://doi.org/10.1007/BF00250320
  30. Javaheri, M., G. E. Jenneman, M. J. McInnemey, and R. M. Knapp (1985), Anaerobic production of a biosurfactant by Bacillus licheniformis JF-2. Appl. Environ. Microbiol. 50, 698-700
  31. Yakimov, M. M., K. N. Timmis, V. Wray, and H. L. Fredrickson (1995), Characterization of a new lipopeptide surfactant produced by thermotolerant and halotolerant subsurface Bacillus licheniformis BAS50. Appl. Environ. Microbiol. 61, 1706-1713
  32. Neu. T. R. and K. Poralla (1990), Emulsifying agent from bacteria isolated during screening for cells with hydrophobic surfaces. Appl. Microbiol. Biotechnol. 32, 521-525 https://doi.org/10.1007/BF00173721
  33. Arima, K., A. Kakinuma, and G. Tamura (1968), Surfactin, a crystalline peptide lipid surfactant produced by Bacillus subtilis : isolation, characterization and its inhibition of fibrin clot formation. Biochem. Biophys. Res. Commun. 31, 488-494 https://doi.org/10.1016/0006-291X(68)90503-2
  34. Bernheimer, A. W. and L. S. Avigad (1970), Nature and properties of a cytological agent produced by Bacillus subtilis. J. Gen. Microbiol. 61, 361-369 https://doi.org/10.1099/00221287-61-3-361
  35. Cooper, D. G., J. E. Zajic, and D. F. Gerson (1978), Production of surface active lipids by Corynebacterium lepus. Appl. Environ. Microbiol. 37, 4-10
  36. Cooper, D. G., S. N. Liss, R. Longay, and J. E. Zajic (1989), Surface activities of Mycobacterium and Pseudomonas. J. Ferment. Technol. 59, 97-101
  37. Desai, A. J., K. M. Patal, and J. D. Desai (1988), Emulsifier production by Pseudomonas fluorescens during the growth on hydrocarbons. Curr. Sci. 57, 500-501
  38. Persson, A., E. Oesterberg, and M. Dostalek (1988), Biosurfactant production by Pseudomonas Fluorescens 378: Growth and product ch따acteristics. Appl. Microbiol. Biotechnol. 29, 1-4 https://doi.org/10.1007/BF00258342