Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
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Inhibitory effect of Smilacis Glabrae Rhizoma on nitric oxide production in the macrophage cell line RAW 264.7

토복령(土茯笭)이 RAW264.7 대식세포주에서 산화질소 억제에 미치는 영향

  • Lee, Kang-Hee (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Jung, Jun-Hee (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Kim, Ee-Hwa (Dept. of Meridian & Acupoint, College of Oriental Medicine, Semyung University) ;
  • Lee, Jae-Hyuk (Dept. of Oriental Neuropsychiatry, College of Oriental Medicine, Semyung University)
  • 이강희 (세명대학교 한의과대학 경혈학교실) ;
  • 정준희 (세명대학교 한의과대학 경혈학교실) ;
  • 김이화 (세명대학교 한의과대학 경혈학교실) ;
  • 이재혁 (세명대학교 한의과대학 한방신경정신과학교실)
  • Published : 2009.09.27
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Abstract

Objectives : 본 연구의 목적은 제습, 해독, 통리관절등의 효능이 있는 토복령이 RAW264.7 대식세포주에서 lipopolysaccharide(LPS)로 처치하여 생성되는 nitric oxide(NO)와 prostaglandin $E_2$($PGE_2$)에 억제작용이 있는지 관찰하고자 하는 것이다. Methods : 토복령이 RAW264.7 대식세포주에 세포독성이 유무를 관찰하기 위하여 농도별 MTT assay를 수행하여 세포생존율을 측정하였다. 또한 LPS로 염증유발된 RAW264.7 대식세포주에서 토복령의 농도별 처치가 NO 및 $PGE_2$ 생성억제에 미치는 영향을 관찰하고자 NO 및 $PGE_2$ assay를 수행하였다. Results : 토복령의 농도별 처치가 RAW264.7 대식세포주에서 세포독성을 유발하는지 MTT assay로 측정한 결과 세포독성은 관찰되지 않았으며, 토복령은 LPS처치로 인하여 증가된 NO 및 $PGE_2$ 생성을 통계학적으로 유의하게 감소시킴을 관찰하였다. Conclusions : 본 연구를 통하여 토복령은 RAW264.7 대식세포주에서 LPS로 유도된NO 및 $PGE_2$ 생성을 효과적으로 억제시킴으로써 추후 염증질환의 치료제로서 가능성을 확인하였다.

Keywords

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