The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of HTE001, an Herbal Formulation, on Electric Stimulation-induced Penile Erection in Rats

전기 자극으로 유발한 음경 발기력 측정 흰쥐 모델에 대한 HTE001의 발기력 상승효과

  • Lim, Dong-Wook (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Dong-Hun (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Song, Mi-Kyoung (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Mi-Yeon (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Bu, Young-Min (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Ho-Cheol (Dept. of Herbal Pharmacology, College of Oriental Medicine, Kyung Hee University)
  • 임동욱 (경희대학교 한의과대학 본초학교실) ;
  • 이동헌 (경희대학교 한의과대학 본초학교실) ;
  • 송미경 (경희대학교 한의과대학 본초학교실) ;
  • 김미연 (경희대학교 한의과대학 본초학교실) ;
  • 부영민 (경희대학교 한의과대학 본초학교실) ;
  • 김호철 (경희대학교 한의과대학 본초학교실)
  • Published : 2009.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study was conducted to investigate the effect of HTE001, a multi-herbal mixture consisting of 10 herbs, Cornus Frutus, Schizandrae Fructus, Rubi Fructus, Cnidi Fructus, Acanthopanacis senticosi Radix, Cinnamomi Cortex, Eucommiae Cortex, Allii Bulbus, Rehmanniae Radix and Ginseng Radix, on electrostimulation-induced penile erection in rats. Methods : Intracavernous pressure (ICP) and mean arterial blood pressure (MAP) were simultaneously monitored through electric stimulation of the cavernous nerve after the oral administration of HTE001 (30, 100, 300 mg/kg) in normal rats. Statistical analysis was performed on maximal intracavernous pressure (ICP), maximal intracavernous pressure/mean arterial blood pressure (ICP/MAP) ratio, and the area under the curve (AUC) of ICP/MAP ratio. Results : Oral administration of HTE001 300 mg/kg caused the ICP to increase in a frequency-dependent manner. And HTE001 300 mg/kg treatment group showed the highest value in the ICP/MAP ratio and the AUC value of the ICP/MAP ratio compared to the control group at 2 Hz, 6 Hz and 10 Hz, respectively without an effect on the mean arterial blood pressure under the same stimulation of the cavernous nerve. Conclusions : These results show that HTE001 improve penile erection and prolong the decay period in normal rats without affecting mean arterial blood pressure, and suggest that HTE001 could be a good therapeutic candidate to treat erectile dysfunction.

Keywords

References

  1. Tharakan B, Manyam BV. Botanical therapies in sexual dysfunction. Phytother Res. 2005 ; 19 : 457-63 https://doi.org/10.1002/ptr.1634
  2. Choi YD, Rha KH, Choi HK. In vitro and in vivo experimental effect of Korean red ginseng on erection. J Urol. 1999 ; 162 : 1508-11 https://doi.org/10.1016/S0022-5347(05)68349-8
  3. Laumann EO, Paik A, Rosen RC. Sexual dysfunction in the United States: prevalence and predictors. JAMA. 1999 ; 281 : 537-44 https://doi.org/10.1001/jama.281.6.537
  4. Feldman HA, Goldstein I, Hatzichristou DG, Krane RJ, McKinlay JB. Impotence and its medical and psychosocial correlates: results of the Massachusetts Male Aging Study. J Urol. 1994 ; 151 : 54-61 https://doi.org/10.1016/S0022-5347(17)34871-1
  5. Johannes CB, Araujo AB, Feldman HA, Derby CA, Kleinman KP, McKinlay J. Incidence of erectile dysfunction in men 40 to 69 years old: longitudinal results from the Massachusetts male aging study. J Urol. 2000 ; 163 : 460-3 https://doi.org/10.1016/S0022-5347(05)67900-1
  6. Ayta IA, McKinlay JB, Krane RJ. The likely worldwide increase in erectile dysfunction between 1995 and 2025 and some possible policy consequences. BJU Int. 1999 ; 84 : 50 https://doi.org/10.1046/j.1464-410x.1999.00142.x
  7. Son HC, Byun SS, Park EC, Cho KS, Jo MK, Kim SW, Kim, HH, Paick J, Lee CW. Prevalence of sexual dysfunction in men older than 40 living in Seoul. Korean Journal of Urology. 2002 : 43, 52-61
  8. Campos AR, Lima RC, Jr Uchoa DE, Silveira ER, Santos FA, Rao VS. Pro-erectile effects of an alkaloidal rich fraction from Aspidosperma ulei root bark in mice. J Ethnopharmacol. 2006 ; 104 : 240-4 https://doi.org/10.1016/j.jep.2005.09.009
  9. Drewes SE, George J, Khan F. Recent findings on natural products with erectile-dysfunction activity. Phytochemistry. 2003 ; 62 : 1019-25 https://doi.org/10.1016/S0031-9422(02)00621-0
  10. Adimoelja A. Phytochemicals and the breakthrough of traditional herbs in the management of sexual dysfunctions. 2000 ; Int J Androl 23 Suppl 2 : 82-4 https://doi.org/10.1046/j.1365-2605.2000.00020.x
  11. Park JY, Shin HK, Lee YJ, Choi YW, Bae SS, Kim CD. The mechanism of vasorelaxation induced by Schisandra chinensis extract in rat thoracic aorta. J Ethnopharmacol. 2009 ; 121 : 69-73 https://doi.org/10.1016/j.jep.2008.09.031
  12. Chen K, Fang J, Kuang X, Mo Q. Effects of the fruit of Rubus chingii Hu on hypothalamuspituitary-sex gland axis in rats. Zhongguo Zhong Yao Za Zhi. 1996 ; 21 : 560-2
  13. Li CS, Zhang GX, Shi TR, Dong XX, Sun P, Li DD, Xu HZ, Chen KJ. Experimental study on effect of the water extract of dogwood fruits on the liver and testis in rats model of kidneyyang deficiency. Zhongguo Zhong Yao Za Zhi. 2003 ; 28 : 743-6
  14. Chen J, Chiou WF, Chen CC, Chen CF. Effect of the plant-extract osthole on the relaxation of rabbit corpus cavernosum tissue in vitro. J Urol. 2000 ; 163 : 1975-80 https://doi.org/10.1016/S0022-5347(05)67613-6
  15. Deyama T, Nishibe S, Nakazawa Y. Constituents and pharmacological effects of Eucommia and Siberian ginseng. Acta Pharmacol Sin. 2001 ; 22 : 1057-70
  16. Lee HS, Kim BS, Kim MK. Suppression effect of Cinnamomum cassia bark-derived component on nitric oxide synthase. J Agric Food Chem. 2002 ; 50 : 700-3
  17. Zhang WH, Li G, Dong HS, Liu ZL, Xiong CL. The effects of eucommia ulmoides oliv on catching action of diabetic rats and myelinated nerve fibers in penile tissues. Zhonghua Nan Ke Xue. 2006 ; 12 : 466-9
  18. Morihara N, Nishihama T, Ushijima M, Ide N, Takeda H, Hayama M. Garlic as an anti-fatigue agent. Mol Nutr Food Res. 2007 ; 51 : 1329-34 https://doi.org/10.1002/mnfr.200700062
  19. Lee BC, Choi JB, Cho HJ, Kim YS. Rehmannia glutinosa ameliorates the progressive renal failure induced by 5/6 nephrectomy. J Ethnopharmacol. 2009 ; 122 : 131-5 https://doi.org/10.1016/j.jep.2008.12.015
  20. Gauthaman K, Adaikan PG, Prasad RN. Aphrodisiac properties of Tribulus Terrestris extract (Protodioscin) in normal and castrated rats. Life Sci. 2002 ; 71 : 1385-96 https://doi.org/10.1016/S0024-3205(02)01858-1
  21. Walker DK, Ackland MJ, James GC, Muirhead GJ, Rance DJ, Wastall P, Wright PA. Pharmacokinetics and metabolism of sildenafil in mouse, rat, rabbit, dog and man. Xenobiotica. 1999 ; 29 : 297-310 https://doi.org/10.1080/004982599238687
  22. Mizusawa H, Ishizuka O, Nishizawa O. Animal models for studying penile hemodynamics. Asian J Androl. 2002 ; 4 : 225-8
  23. Andersson KE, Wagner G. Physiology of penile erection. Physiol Rev. 1995 ; 75 : 191-236
  24. Chen KK, Chan JY, Chang LS, Chen MT, Chan SH. Intracavernous pressure as an experimental index in a rat model for the evaluation of penile erection. J Urol. 1999 ; 147 : 1124-8
  25. Quinlan DM, Nelson RJ, Partin AW, Mostwin JL, Walsh PC. The rat as a model for the study of penile erection. J Urol. 1989 ; 141 : 656-61 https://doi.org/10.1016/S0022-5347(17)40926-8
  26. Gemalmaz H, Waldeck K, Chapman TN, Tuttle JB, Steers WD, Andersson KE. In vivo and in vitro investigation of the effects of sildenafil on rat cavernous smooth muscle. J Urol. 2001 ; 165 : 1010-4 https://doi.org/10.1016/S0022-5347(05)66593-7
  27. Yu JY, Kang KK, Yoo M, Kwon JW. Penile erectile responses to electric stimulation are enhanced by a new phosphodiesterase type-5 inhibitor. Int J Urol. 2005 ; 12 : 299-304 https://doi.org/10.1111/j.1442-2042.2005.01039.x
  28. Ueno N, Iwamoto Y, Segawa N, Kinoshita M, Ueda H, Katsuoka Y. The effect of sildenafil on electrostimulation-induced erection in the rat model. Int J Impot Res. 2002 ; 14 : 251-5 https://doi.org/10.1038/sj.ijir.3900860
  29. Wada Y, Kikuchi K, Imamura T. A fatal hypotension by sildenafil in an end-stage renal disease patient with hypertension and abnormal pharmacokinetics of the medicine. Nephrology (Carlton). 2009 ; 14 : 357-8 https://doi.org/10.1111/j.1440-1797.2009.01132.x