Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibitory Effects of 23 Korean Local Plant Extracts on Aldo-keto reductase family 1 B10 (AKR 1 B10)

한국 자생식물 추출물 23종의 Aldo-keto reductase family 1 B10 (AKR 1 B10) 효소 억제효과

  • Lee, Joo-Young (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute) ;
  • Song, Dae-Geun (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute) ;
  • Jung, Sang-Hoon (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute) ;
  • Kim, Jong-Hwan (Specialty Crops Experiment Station, Gangwondo Agricultural Research and Extension Services) ;
  • Ahn, Soo-Young (Specialty Crops Experiment Station, Gangwondo Agricultural Research and Extension Services) ;
  • Nho, Chu-Won (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute) ;
  • Pan, Cheol-Ho (Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute)
  • 이주영 (한국과학기술연구원 강릉분원) ;
  • 송대근 (한국과학기술연구원 강릉분원) ;
  • 정상훈 (한국과학기술연구원 강릉분원) ;
  • 김종환 (강원도 농업기술원 특화작물시험장) ;
  • 안수용 (강원도 농업기술원 특화작물시험장) ;
  • 노주원 (한국과학기술연구원 강릉분원) ;
  • 판철호 (한국과학기술연구원 강릉분원)
  • Published : 2009.09.30
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Abstract

We examined ethanol extracts prepared from 23 Korean local plants obtained in Pyeongchang, Gangwon-do for their inhibitory effects on recombinant human AKR 1 B10 (rhAKR1B10) in vitro. To do this, rhAKR1B10 was first expressed in E. coli as a biological active form and purified by using Ni-affinity chromatography followed by gel permeation chromatography. Then, rhAKR1B10 was used for screening out 23 Korean local plant extracts having an inhibitory activity against itself. Among them, six extracts showed more than 50% inhibition of rhAKR1B10 activity at the concentration of $10{\mu}g$/ml. Especially, the extracts of Ligularia fischeri var. spiciformis Nakai and Rhus trichocarpa Miq. were the most potent because their $IC_{50}$ values were 2.94 and $2.00{\mu}g$/ml, respectively.

Keywords

References

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