Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Water Extracts of Cuscuta Japonica Chois in RANKL-induced Osteoclast Differentiation

파골세포 분화에서 토사자 물 추출물의 효과

  • Cho, Hae-Joong (Department of Obstetrics and Gynecology, School of Medicine, Wonkwang University) ;
  • Choi, Min-Kyu (Department of Anatomy, School of Medicine, Wonkwang University) ;
  • Kim, Jeong-Joong (Department of Anatomy, School of Medicine, Wonkwang University) ;
  • Le, Yan (Department of Anatomy, School of Medicine, Wonkwang University) ;
  • Song, Jeong-Hoon (Department of Plastic Surgery, School of Medicine, Wonkwang University) ;
  • Lee, Myeung-Su (Department of Rheumatology, School of Medicine, Wonkwang University) ;
  • Lee, Chang-Hoon (Department of Rheumatology, School of Medicine, Wonkwang University) ;
  • Jang, Sung-Jo (Department of Neurosurgery, School of Medicine, Wonkwang University) ;
  • Kwak, Han-Bok (Department of Anatomy, School of Medicine, Wonkwang University) ;
  • Oh, Jae-Min (Department of Anatomy, School of Medicine, Wonkwang University)
  • 조해중 (원광대학교 의과대학 산부인과학교실) ;
  • 최민규 (원광대학교 의과대학 해부학교실) ;
  • 김정중 (원광대학교 의과대학 해부학교실) ;
  • 리연 (원광대학교 의과대학 해부학교실) ;
  • 송정훈 (원광대학교 의과대학 성형외과학교실) ;
  • 이명수 (원광대학교 의과대학 류마티스학교실) ;
  • 이창훈 (원광대학교 의과대학 류마티스학교실) ;
  • 장성조 (원광대학교 의과대학 신경외과학교실) ;
  • 곽한복 (원광대학교 의과대학 해부학교실) ;
  • 오재민 (원광대학교 의과대학 해부학교실)
  • Published : 2009.08.25
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Abstract

Osteoclasts are bone-resorbing multinucleated cells derived from the monocyte/macrophage lineage. The differentiation of osteoclasts are regulated by osteoblastic cells expressed RANKL, which is the most critical molecule for osteoclast differentiation. In this study, we found that water extracts of cuscuta inhibited RANKL-mediated osteoclast differentiation by direct action on bone marrow macrophages (BMMs) without cytotoxicity. In BMMs, water extracts of cuscuta inhibited the mRNA expression of c-Fos, NFATc1, TRAP, and OSCAR. Also, the protein expression of c-Fos and NFATc1 was inhibited by water extracts of cuscuta treatement. Water extracts of cuscuta inhibited the phosphorylation of p38, ERK, and JNK in BMMs treated with RANKL. However, water extracts of cuscuta did not inhibit RANKL-induced I-${\kappa}B$ activation. Water extract of cuscuta failed to inhibit bone resorption by osteoclasts cultured on hydroxyapatite plates. These results suggest that cuscuta may be a promising drug for use against bone disorders such as osteoporosis and rheumatoid arthritis.

Keywords

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