Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Anti-cancer Activities of Extract from the Bark of Melia azedarach L. var. japonica Makino

고련피 추출물의 항암활성

  • Kim, Hyun-Woo (Research Institute for Natural Product and Medicines, Semyung University) ;
  • Kang, Se-Chan (Research Institute for Natural Product and Medicines, Semyung University)
  • 김현우 (세명대학교 천연자원의약연구소) ;
  • 강세찬 (세명대학교 천연자원의약연구소)
  • Published : 2009.08.30
Download PDF
⟨ Previous Next ⟩

Abstract

In the present study, the anti-cancer activity of 80% ethanol extracts from 120 kinds of medicinal herbs and native plants were investigated. Among them, the barks of Melia azedarach L. var. japonica Makino showed the highest cytotoxicity in HCT-15 human colon cancer cell. With this result, we carried out hollow fiber (HF) assay and anti-metastasis study to confirm the anti-cancer effects of M. azedarach var. japonica. In MTT assay, M. azedarach var. japonica.inhibited the proliferation of HCT-15 cells in dose-dependent manner. HF assay was carried out using A549 human adenocarcinoma cell, HCT-15 and SK-Hep1 human liver cancer cell via intraperitoneal (IP) and subcutaneous (SC) site. As a results, SK-Hep1 implanted in IP site showed the highest cytotoxicity. The result from metastatic model using B16/BL6 mouse corresponded to that of HF assay. These results suggest that the ethanol extract from M. azedarach var. japonica. might have a potent anti-cancer activity and advanced study is needed for the development of novel natural anti-cancer drug.

Keywords

References

  1. Baguley, B.C. and Nash, R. 1981. Antitumour activity of substituted 9-anilinoacridines-comparison of in vivo and in vitro testing systems. Eur. J. Cancer 17:671-679 https://doi.org/10.1016/0014-2964(81)90271-1
  2. Boyd, M.R. 1989. Status of the NCI preclinical antitumor drug discovery screen. Principles and Practice of Oncology 3:1-12
  3. Bridges, E.M., Bibby, M.C. and Burchill, S.A. 2006. The hollow fiber assay for drug responsiveness in the ewing's sarcoma family of tumors. J. Pediatr. 149:103-111 https://doi.org/10.1016/j.jpeds.2006.02.042
  4. Casciari, J.J., Hollingshead, M.G., Alley, M.C., Mayo, J.G. and Malspeis, L. 1994. Growth and chemotherapeutic response of cells in a hollow-fiber in vitro solid tumor model. J. Natl. Cancer Inst. 86:1846-1852 https://doi.org/10.1093/jnci/86.24.1846
  5. Cespedes, C.L., Alarcon, J., Aranda, E., Becerra, J. and Silva, M. 2001. Insect growth regulator and insecticidal activity of beta-dihydroagarofurans from Maytenus spp. (Celastraceae). Z. Naturforsch C. 56:603-613
  6. Cha, S.M. 1977. Potential anticancer medicinal plants. A statistical evaluation of their frequencies of appearance in oriental medicine formularies. Kor. J. Pharmacogn. 8:1-15
  7. Denizot, F. and Lang, R. 1986. Rapid colorimetric assay for cell growth and survival. Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89:271-277 https://doi.org/10.1016/0022-1759(86)90368-6
  8. Geran, R.I., Greenberg, N.H., Macdonald, M.M. and Abbott, B.J. 1977. Modified protocol for the testing of new synthetics in the L1210 lymphoid leukemia murine model in the DR&D program, DCT, NCI. Natl. Cancer Inst. Monogr. 45:151-153
  9. Holbeck, S.L. 2004. Update on NCI in vitro drug screen utilities. Eur. J. Cancer 40:785-793 https://doi.org/10.1016/j.ejca.2003.11.022
  10. Hollingshead, M.C., Alley, M.C., Camalier, R.F., Abbot, B.J. and Mayo, J.G. 1995. In vivo cultivation of tumor cells in hollow fibers. Life Sci. 57:131-141 https://doi.org/10.1016/0024-3205(95)00254-4
  11. Hong, M.H. 1972. Statistical studies on the formularies of oriental medicine(1) prescription frequency and their origin distribution of herb drugs. Kor. J. Pharmacogn. 3:57-64
  12. Hwang, W.I., Lee, S.D. and Oh, S.K. 1982. A study on the pharmacological activities of Korean medicinal herbs mamly on the antitumor activities (in Korean). Kor. Biochem. J. 15:205-219
  13. Hwang, W.I. and Oh, S.K. 1984. Effects of petroleum ether extract of ginseng root on same enzyme activity in human colon cancer cells. Kor. J. Ginseng Sci. 8:153-166
  14. Itokawa, H., Qiao, Z.S., Hirobe, C. and Takeya, K. 1995. Cytotoxic limonoids and tetranortriterpenoids from Melia azedarach. Chem. Pharm. Bull. 43:1171-1175 https://doi.org/10.1248/cpb.43.1171
  15. Keshri, G., Lakshmi, V. and Singh, M.M. 2003. Pregnancy interceptive activity of Melia azedarach Linn. in adult female Sprague-Dawley rats. Contraception 68:303-306 https://doi.org/10.1016/S0010-7824(03)00143-4
  16. Khan, M.R., Kihara, M. and Omoloso, A.D. 2001. Antimicrobial activity of Horsfieldia helwigii and Melia azedarach. Fitoterapia 72:423-427 https://doi.org/10.1016/S0367-326X(00)00334-8
  17. Kwon, H.C., Lee, B.G., Kim, S.H., Jung, C.M. and Hong, S.Y. 1999. Inducible nitric oxide synthase inhibitors from Melia azedarach var. japonica. Arch. Pharm. Res. 22:410-413 https://doi.org/10.1007/BF02979067
  18. Lee, B.G., Kim, S.H., Zee, O.P., Lee, K.R. and Lee, H.Y. 2000. Suppression of inducible nitric oxide synthase expression in RAW 264. 7 macrophages by two beta-carboline alkaloids extracted from Melia azedarach. Eur. J. Pharmacol. 406:301-309 https://doi.org/10.1016/S0014-2999(00)00680-4
  19. Lee, J.Y., Hwang, W.I. and Lim, S.T. 1998. Effects of Platycodon grandiflorum DC. extract on the growth of cancer cell lines. Kor. J. Food Sci. Technol. 30:13-21
  20. Miyazaki, T. and Nishijima, M. 1981. Studies on fungal polysaccharides. XXVII. Structural examination of a water-soluble, antitumor polysaccharide of Ganoderma lucidum. Chem. Pharm. Bull. 29:3611-3616 https://doi.org/10.1248/cpb.29.3611
  21. Ofulla, A.V., Chege, G.M., Rukunga, G.M., Kiarie, F.K. and Githure, J.I. 1995. In vitro antimalarial activity of extracts of Albizia gummifera, Aspilia mossambicensis, Melia azedarach and Azadirachta indica against Plasmodium falciparum. Afr. J. Health Sci. 2:309-311
  22. Plowman, J., Camalier, R., Alley, M., Sausville, E. and Schepartz, S. 1999. US NCI testing procedures. In: Fiebig H-H, Burger AM, editors. Contributions in oncology:relevance of tumour models for anticancer drug development. Basel: Karger pp. 121-135
  23. Son, H.S. and Hwang, W.I. 1990. A study on the cytotoxic activity of garlic (Allium sativum) extract against cancer cells (in Korean). Kor. J. Nutrition 23:135-147
  24. Takeya, K., Quio, Z.S., Hirobe, C. and Itokawa, H. 1996a. Cytotoxic trichilin-type limonoids from Melia azedarach. Bioorg. Med. Chem. 4:1355-1359 https://doi.org/10.1016/0968-0896(96)00128-9
  25. Takeya, K., Qiao, Z.S., Hirobe, C. and Itokawa, H. 1996b. Cytotoxic azadirachtin-type limonoids from Melia azedarach. Phytochemistry 42:709-712 https://doi.org/10.1016/0031-9422(96)00044-1
  26. Thayer, P.S., Himmelfarb, P. and Watts, G.L. 1971. Cytotoxicity assays with L1210 cells in vitro: comparison with L1210 in vivo and KB cells in vitro. Cancer Chemother. Rep. 2:21-25