Development and Reproduction (한국발생생물학회지:발생과생식)

The Korean Society of Developmental Biology (한국발생생물학회)
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Spermatogenesis and Ultrastructural Characteristics of Spermatozoa of Brackish Water Diploid Clam, Corbicula japonica (Bivalvia: Corbiculidae)

기수산 2배체 재첩, Corbicula japonica(Bivalvia: Corbiculidae)의 정자형성과정 및 정자의 미세구조적 특징

  • Jun, Je-Cheon (West Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Kim, Bong-Seok (Dept. of Biotechnology, National Fisheries Research and Development Institute) ;
  • Chung, Ee-Yung (Korea Marine Environment & Ecosystem Institute, Dive Korea) ;
  • Kim, Jin-Hee (Korea Inter-University Institute of Ocean Science, Pukyong National University) ;
  • Park, Gab-Man (Dept. of Parasitology, Kwandong University College of Medicine) ;
  • Park, Sung-Woo (Dept. of Aquatic Life Medicine, Kunsan National University)
  • 전제천 (국립수산과학원 서해수산연구소) ;
  • 김봉석 (국립수산과학원 생명공학연구소) ;
  • 정의영 (다이브코리아 해양환경생태연구소) ;
  • 김진희 (부경대학교 해양과학공동연구소) ;
  • 박갑만 (관동대학교 의과대학 기생충학교실) ;
  • 박성우 (군산대학교 수산생명의학과)
  • Published : 2009.06.30
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Abstract

Spermatogenesis and ultrastructural characteristics of sperm of brackish water diploid Corbicula japonica were investigated by electron microscope observations. Based on the cytological studies, the spermatozoon of this species (brackish water diploid) C japonica is approximately 55 ${\mu}m$ in length. The sperm head (about 12 ${\mu}m$ long) is elongated and tapers with a slight curve. Sperm nucleus is about 7.90 ${\mu}m$ long, and the acrosome is about 2.70 ${\mu}m$ long: The morphologies of the sperm nucleus type and the acrosome shape of this species are a long arrow-like type and long cone-like shape, respectively. The sperm head of this species (external fertilization, dioecious and oviparous species) is partially modified from that of the primitive type, as seen in triploid Corbicula species (internal fertilization, hermaphrodite and ovoviparous species), reported by some authors. However, this species produces uniflagellate spermatozoa, unlike freshwater triploid hermaphroditic clams being possessed of partially modified biflagellate spermatozoa. Diploid C japonica is similar to those of other bivalves being possessed of a short midpiece containing four mitochondria surrounding the centrioles. The axoneme of the sperm tail flagellum consists of nine pairs of microtubules at the periphery and a pair at the center. The axoneme of the sperm tail shows a 9+2 structure, and from uniflagellate sperm cross sectioned, in particular, wing-like axonernal lateral fins are observed, as seen in external fertilization fishes.

기수산 2배체 일본재첩(Corbicula japonica)의 정자형성과정 및 정자의 미세 구조적 특징을 전자현미경 관찰에 의해 조사하였다. 세포학적 조사 결과, 기수산 2배체인 일본재첩의 정자 길이는 약 55${\mu}m$이다. 정자 두부(길이 약 12${\mu}m$)는 길게 신장되어 있으며 약간 구부러져 있다. 정핵 길이는 7.90 ${\mu}m$, 첨체 길이는 약 2.70 ${\mu}m$이다. 정자의 핵과 첨체의 형태는 각각 긴 화살 모양과 길다란 원추 모양을 나타낸다. 본 종(체외수정, 자웅이체, 난생종)의 정자 두부는 이미 몇몇 저자들에 의해서 보고된 3배체 재첩류(체내수정, 자웅동체, 난태생종)의 정자 두부에서 나타나는, 원시형으로부터 부분적으로 변형된 형태를 나타내고 있다. 그러나 부분적으로 변형된 2개의 편모가 있는 정자를 가지는 담수산 3배체인 자웅동체 조개류와 달리 한 개의 편모를 갖는 정자를 본 종은 생산한다. 2배체 일본재첩은 중심체를 둘러싸는 4개의 미토콘드리아를 가지고 있어, 짧은 중편을 가지는 다른 이매패류의 것들과 유사하다. 정자 미부 편모의 약소님은 중앙에 1쌍의 미세소관과 주변에 9쌍의 미세소관으로 구성되어 있다. 정자 미부의 악소님은 9+2구조를 가지며, 횡절단된 한 개의 편모를 갖는 정자에서 특히, 체외수정 어류들에서 나타는 날개 모야으이 악소님 lateral fin들이 관찰되었다.

Keywords

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