Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
권호 표지

Feasibility Study on RI Biochip Application to Detection of Risk Factors of Atherosclerosis

RI검출 바이오칩의 혈관계 질환 발생 위험인자 검지에 대한 타당성 연구

  • Ko, Kyong-Cheol (Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Choi, Mi Hee (Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Park, Sang Hyun (Radiation Research Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Cho, Kyung-Hyun (School of Biotechnology, Yeungnam University) ;
  • Lee, Ki-Teak (Department of Food Science and Technology, Chungnam National University)
  • 고경철 (한국원자력연구원 방사선과학연구소 방사선생명공학연구부) ;
  • 최미희 (한국원자력연구원 방사선과학연구소 방사선생명공학연구부) ;
  • 박상현 (한국원자력연구원 방사선과학연구소 방사선생명공학연구부) ;
  • 조경현 (영남대학교 생명공학부) ;
  • 이기택 (충남대학교 식품공학과)
  • Received : 2009.02.05
  • Accepted : 2009.03.16
  • Published : 2009.03.31

⟨ Previous Next ⟩

Abstract

Microarrays can be used to screen thousands of binding events in a parallel and high throughput fashion and are of major importance in disease diagnosis and drug discovery. The use of radioisotope is conventionally regarded as one of the most sensitive detection methods. Atherosclerosis is a common disorder affecting arterial blood vessels. It happens when fat, cholesterol, and other substances made in the arterial blood vessels form a hard substances called plaque. Lipoprotein-associated phospholipase $A_2$ ($Lp-PLA_2$), a phospholipase $A_2$ enzyme, is used as a marker for cardiac disease. The detection of $Lp-PLA_2$ was accomplished by using radioactive [$^3H-acetyl$] PAF as a substrate and a feasibility study on RI biochip application to detection of $Lp-PLA_2$, a risk factors of atherosclerosis was performed. Inhibitive activity of a native plant extract was also determined by using the RI biochip. It was found to be applicable to a high-throughput screening of inhibitors for developing atherosclerosis therapeutic agents.

Keywords

Acknowledgement

Supported by : 교육과학기술부