Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Effect of High Temperature and High Humidity on Protein Expression and Plasma Membrane $H^{+}ATPase$ of Umbel with Flower of Onion (Allium cepa L.)

고온과 고습 조건하에서 양파 화구의 총 단백질의 발현과 원형질막 $H^{+}ATPase$의 영향

  • Ku, Yang-Gyu (Department of Plant Biotechnology, Bioenergy Research Institute, College of Agriculture and Life Science, Chonnam National University) ;
  • Park, Won (Department of Plant Biotechnology, Bioenergy Research Institute, College of Agriculture and Life Science, Chonnam National University) ;
  • Lee, Eul-Tai (Mokpo Sub-Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Kim, Cheol-Woo (Mokpo Sub-Institute, National Institute of Horticultural & Herbal Science, Rural Development Administration) ;
  • Oh, Jeong-Min (Department of Plant Biotechnology, Bioenergy Research Institute, College of Agriculture and Life Science, Chonnam National University) ;
  • Jang, Young-Seok (Bioenergy Crop Research Center, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Yong-Kwon (National Agricultural Cooperative Federation, Seed Research & Development Center) ;
  • Ahn, Sung-Ju (Department of Plant Biotechnology, Bioenergy Research Institute, College of Agriculture and Life Science, Chonnam National University)
  • 구양규 (전남대학교 농업생명과학대학 식물생명공학부 바이오에너지연구소) ;
  • 박원 (전남대학교 농업생명과학대학 식물생명공학부 바이오에너지연구소) ;
  • 이을태 (국립원예특작과학원 목포출장소) ;
  • 김철우 (국립원예특작과학원 목포출장소) ;
  • 오정민 (전남대학교 농업생명과학대학 식물생명공학부 바이오에너지연구소) ;
  • 장영석 (국립식량과학원 바이오에너지작물센터) ;
  • 김용권 (농협중앙회 종묘개발센터) ;
  • 안성주 (전남대학교 농업생명과학대학 식물생명공학부 바이오에너지연구소)
  • Published : 2009.06.30
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Abstract

This study was undertaken the effect of high temperature and high humidity on protein expression and especially plasma membrane (PM) $H^{+}ATPase$ of umbel with flowers of early cultivar 'Shinsunhwang' and intermediate cultivar 'Maebsihwang' of onion (Allium cepa L.). There were no visible any difference on the protein pattern from before flowering stage to full flowering stage of two onion cultivars, however, seed set stages were revealed induced/deduced protein patterns. At day 18, protein expression pattern of the high temperature and high humidity treatments of two cultivars was significantly reduced compared to controls. Furthermore, various protein expression of the high temperature treatment was more reduced compared to high humidity treatment. PM $H^{+}ATPase$ expression of the control plants of two onion cultivars was clearly shown, but was not detectable under high temperature treatment of the two onion cultivars using western blot analysis. PM $H^{+}ATPase$ expression of the high humidity treatment was faintly detected intermediate cultivar 'Maebsihwang', not early cultivar 'Shinsunhwang'. These results indicate that protein expression pattern and PM $H^{+}ATPase$ under high temperature treatment was considered to be more damaged compared to high humidity.

본 연구는 고온과 고습 조건 하에서 양파 화구의 총 단백질 발현과 원형질막 $H^{+}ATPase$ 영향을 조사하고자 조생종 '신선황'과 중생종 '맵시황'를 이용하여 실험을 수행하였다. '신선황'과 '맵시황' 품종의 양파 회구 개화전, 반개화, 그리고 만개 단계에서 단백질의 양생에는 차이를 보이지 않았지만 결실 단계에서는 유도 및 비유도되는 단백질이 현저하게 나타났다. 양파 화구 개화 후 실시한 고온과 고습처리 18일째의 양파두 품종의 단벡질도 현저하게 감소하였고 특히 고온처리구에서 더 감소되는 경향을 보였다. 원형절막 $H^{+}ATPase$ 발현을 western-blot으로 살펴본 결과, '신선황' 과 '맵시황'의 경우 대조구에서 원형질막 $H^{+}ATPase$ 단백질 발현이 유지 되는 반면에, 고온처리구에서는 두품종 모두 원형질막 $H^{+}ATPase$가 발현 되지 않았다. 고습처리구에서 중생종 '맵시황'약 원형질막 $H^{+}ATPase$는 발현 되었는데 조생종 '신선황'에서 발현되지 않았다. 이는 양파 종자 성숙단계에서 고온 조건을 조우하면 고습처리 보다는 단백질 및 원형질막의 $H^{+}ATPase$피해가 더 심각함을 보여 주는 결과이다.

Keywords

References

  1. Ahn, S.J., M. Sivaguru, G.C. Chung, Z. ReIgel, and H. Matsumoto. 2002. Aluminum-induced growth inhibition is associated with impaired efflux and influx $H^{+}$ across the plasma membrane in root apices of squash (Cucurbita pepo). J. Exp.Bot. 53: 1959-1966 https://doi.org/10.1093/jxb/erf049
  2. Ahn, S.J., M. Sivaguru, H. Osawa, G.C. Chung, and H. Matsumoto. 2001. Aluminum inhibits $H^{+}$-ATPase activity by pennanently altering the plasma membrane surface potentials in squash roots. Plant Physiol. 126: 1381-1390 https://doi.org/10.1104/pp.126.4.1381
  3. Ahn, S.J., Y.S. Kim, W. Park, Y.G. Ku, K.S. Min, and T.I. Hwang. 2007. Aluminum-induced root growth inhibition and impaired plasma membrane $H^{+}$-flux inmung bean. Korean J. Crop. Sci. 52(2): 213-219 (in Korean)
  4. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254 https://doi.org/10.1016/0003-2697(76)90527-3
  5. Huh, E.J., K.S. Cho, Y.S. Kwon, and J.G. Woo. 2002. Effects of temperature and photoperiod on bulbing and maturity of spring sown onions in highland. J. Kor. Soc. Hort. Sci. 43(5):587-590 (in Korean)
  6. Jang. Y.S., D.Y. Hyun, C.W. Kim, E.T. Lee, S.K. Cho, I.H. Choi, and B.C. Jeong. 2002. Artificial rearing method of Lucilia illustris for pollination vector in onion (Allium cepa L.). J. Kor. Soc. Hort. Sci. 43 (2): 178-182 (in Korean)
  7. Ku, Y.G, W. Park, E.T. Lee, C.W. Kim, Y.S. Kim, Y.S. Jang, and S.J. Ahn. 2008. Effect of high temperature and humidity on seed production and mother bulb harvesting on onion. Kor. J. Hort. Sci. Technol. 26(2):97-100 (in Korean)
  8. Kumar, S., M. Imtiyaz, and A. Kumar. 2007. Effect of differential soil moisture and nutrient regimes on postharvest attributes of onion (Allium cepa L.). Sci. Hort. 112:121-129 https://doi.org/10.1016/j.scienta.2006.12.024
  9. Lee, E.J., J.K. Jeon, and J.K. Suh. 2008. Effect of supplemental lighting in different lighting Intensity on Pyruvic acid and sugar content in onion (Allium cepa L.). J. Bio-Environ. Control 17(4):266-272
  10. Lim, C.H., T.H. Park, J.L. Cho, and S.S. Kang. 2002. Effect of day length and temperature after bolting on flower curd and seed yield of early onion 'Sarnnamjosaeng'. Kor. J. Hort. Sci & Technol. 20:306-308 (in Korean)
  11. Pathak, C.S. 2000. Hybrid seed production in onion. Journal of New Seeds. 1:89-108 https://doi.org/10.1300/J153v01n03_04
  12. Palmgren, M.G, P. Askerlund, K. Fredrikson, S. Widell, M. Sommarin, and C. Larsson. 1990. Sealed inside-out and right-side-out plasma membrane vesicles: optimal conditions for formation and separation. Plant Physiol. 92:871-880 https://doi.org/10.1104/pp.92.4.871
  13. Ramin A.A. 1999. Storage potential of bulb onions (Allium cepa L.) under high temperatures. J. Hort. Sci. & Biotech. 74:181-186 https://doi.org/10.1080/14620316.1999.11511092
  14. Spurr, C.J., D.A. Fulton, P.H. Brown, and R.I. Clark. 2002. Changes in seed yield and quality with maturity in onion (Allium cepa L., cv. 'Early Cream Gold'). J. Agro. & Crop Sci. 188:275-280 https://doi.org/10.1046/j.1439-037X.2002.00573.x
  15. Sussman, M.R. 1994. Molecular analysis of proteins in the plant plasma membrane. Annu. Rev. Plant Physiol. Plant Mol. Biol. 45:211-234 https://doi.org/10.1146/annurev.pp.45.060194.001235