Journal of the Korean Association of Oral and Maxillofacial Surgeons

  • 제35권3호
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  • Pages.137-145
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  • 2009
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  • 2234-7550(pISSN)
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  • 2234-5930(eISSN)
대한구강악안면외과학회 (The Korean Association of Oral and Maxillofacial Surgeons)
권호 표지

Matrix metalloproteinase(MMP) sensitive hyaluronic acid hydrogel-nanoparticle complex와 rhBMP-2를 이용한 골재생

BONE REGENERATION WITH MMP SENSITIVE HYALURONIC ACID-BASED HYDROGEL, rhBMP-2 AND NANOPARTICLES IN RAT CALVARIAL CRITICAL SIZE DEFECT(CSD) MODEL

  • 남정훈 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 박종철 (서울대학교 치과대학 구강악안면외과학교실) ;
  • 유상배 (서울대학교 치과대학 치학연구소) ;
  • 정용일 (광주 과학기술원 신소재공학과) ;
  • 태기융 (광주 과학기술원 신소재공학과) ;
  • 김정주 (고려대학교 의과대학 의공학교실, BK21 의공학사업단, 한국인공장기센터) ;
  • 박용두 (고려대학교 의과대학 의공학교실, BK21 의공학사업단, 한국인공장기센터) ;
  • 장정원 (서울대학교 치과대학 치학연구소) ;
  • 이종호 (서울대학교 치과대학 구강악안면외과학교실)
  • Nam, Jeong-Hun (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Park, Jong-Chul (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University) ;
  • Yu, Sang-Bae (Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Chung, Yong-Il (Department of Materials Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Tae, Gi-Yoong (Department of Materials Science and Engineering, Gwangju Institute of Science and Technology) ;
  • Kim, Jung-Ju (Department of Biomedical Engineering, Biomedical Engineering, Brain Korea 21 2nd Project for Biomedical Science) ;
  • Park, Yong-Doo (Department of Biomedical Engineering, Biomedical Engineering, Brain Korea 21 2nd Project for Biomedical Science) ;
  • Jahng, Jeong-Won (Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Lee, Jong-Ho (Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University)
  • 발행 : 2009.06.30
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초록

As an efficient controlled release system for rhBMP-2, a functional nanoparticle-hydrogel complex, incorporated with matrix metalloproteinase(MMP) sensitive peptide cross-linker, was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase(ALP) activity and mineral contents analysis, based on the rat calvarial critical size defect(8mm in diameter) model. Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded MMP sensitive hyaluronic acid(HA) based hydrogel-Nanoparticles(NP) complex, as compared to only MMP HA, the MMP HA-NP without rhBMP-2, or even with the rhBMP-2. These improvements included the formation pattern of bone and functional marrow, the degree of calcium quantification, and the ALP activity. These results indicate that the MMP sensitive HA with nano-particle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced rhBMP-2 scaffold.

키워드

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