The Journal of Korean Physical Therapy

The Korean Society of Physical Therapy (대한물리치료학회)
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Expression of MCP-1 in Spinal Dorsal Horn in a Rat Model of Lumbar Disc Herniation

추간판 탈출증 흰 쥐 모델에서 척수후각에서의 MCP-1의 발현

  • Cho, Yun-Woo (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University) ;
  • Park, Hea-Woon (Department of Rehabilitation Medicine, School of Medicine, Catholic University of Deagu) ;
  • Kim, Su-Jeong (Institute of Medical Science, Yeungnam University) ;
  • Seo, Jeong-Min (Institute of Biomedical Engineering, Yeungnam University) ;
  • Do, Sung-Jin (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University) ;
  • Ahn, Sang-Ho (Department of Rehabilitation Medicine, College of Medicine, Yeungnam University)
  • 조윤우 (영남대학교 의과대학 재활의학교실) ;
  • 박해운 (대구가톨릭대학교 의과대학 재활의학교실) ;
  • 김수정 (영남대학교 의과학연구소) ;
  • 서정민 (영남대학교 의료공학연구소) ;
  • 도성진 (영남대학교 의과대학 재활의학교실) ;
  • 안상호 (영남대학교 의과대학 재활의학교실)
  • Received : 2009.08.16
  • Accepted : 2009.09.11
  • Published : 2009.09.25
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Abstract

Purpose: This study evaluates MCP-1 expression in the dorsal horn of a rat model of lumbar disc herniation by an autograft of the nucleus pulposus to the spinal nerve. Methods: After a coccygeal nucleus pulposus graft to the left $5^{th}$ lumbar spinal nerve, proximal to dorsal root ganglion, mechanical allodynia and thermal hyperalgesia were assessed 1 day before surgery, and 1, 10, 20, 30 days after surgery. The mRNA of MCP-1 in the dorsal horn was assessed by real time PCR to compare the temporal pattern of neuropathic pain of the lumbar disc herniation model. Results: In the ipsilateral side of the lumbar disc herniation models, mechanical allodynia and thermal hyperalgesia reached a maximum at 10 days after surgery with significant difference from the control group. Pain was also provoked in the contralateral side of the lumbar disc herniation models with less intensity than the ipsilateral side. The level of MCP-1 mRNA expression in the dorsal horn reached a peak at 20 days after surgery. Conclusion: Mechanical allodynia and thermal hyperalgesia was induced by nucleus pulposus in a rat lumbar disc herniation model, similar to a previously reported peripheral nerve injury model. The level of MCP-1 expression was higher in the dorsal horn of the ipsilateral and contralateral sides. These results suggest that MCP-1 might play a role in the maintenance of neuropathic pain.

Keywords

References

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