The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Anti-inflammatory Effect of LFR on LPS-stimulated THP-1 Cells

  • Sul, Mu-Chang (Dept. of Oriental Internal Medicine, Jaseng Hospital of Orient Medicine) ;
  • Kim, Hong-Yeoul (Dept. of Biochemistry, College of Oriental Medicine, Kyung-hee University) ;
  • Roh, Young-Lae (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-hee University) ;
  • Jung, Hee-Jae (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-hee University) ;
  • Jung, Sung-Ki (Division of Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung-hee University)
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  • ;
  • ;
  • ;
  • 정승기 (경희의료원 부속한방병원 한방5내과 의사실)
  • Published : 2009.06.30
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Abstract

Background and Objective: Luffae Fructus Retinervus (LFR) is used for investigating symptoms of inflammation. We have evaluated the anti-inflammatory effect of LFR by analyzing the expression of pro-inflammatory cytokines. Materials and Methods : We differentiated THP-l cells into macrophage-like cells by treatment with PMA. Inflammation was induced by treatment with LPS and PMA. We determined the safe concentration of LFR by using the MTS and MTT assays and using PD 98059 as a negative control for comparison of the anti-inflammatory effect of LFR. Results : The MTS and MTT analysis showed that the cell survival rate was >80% within the LFR concentration range of 10-100 ng/ml and began to decrease to >80% at 1 ${\mu}g/ml$. By RT-PCR analysis, the gene expression of TNF-${\alpha}$, IL-8, TGF-${\beta}$, IL-6, IL-${\beta}$1, and IL-10 levels were down-regulated when monocyte-derived macrophages were treated with concentrations of LFR between 10 ng/mL and 100 ng/mL. Conclusion : We conclude that LFR exerts an anti-inflammatory effect by inhibiting the expression of pro-inflammatory activity. The results suggest a promising way to treat general inflammatory diseases.

Keywords

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