한국균학회지 (The Korean Journal of Mycology)

  • 제37권1호
  • /
  • Pages.114-116
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  • 2009
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  • 0253-651X(pISSN)
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  • 2383-5249(eISSN)
한국균학회 (The Korean Society of Mycology)
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고추 역병균(Phytophthora capsici)의 액체배지에서 난포자 형성과 발아

Oospore Production in Broth Media and Oospore Germination of Phytophthora capsici

  • 김병섭 (강릉원주대학교 식물생명과학과) ;
  • ;
  • Kim, Byung-Sup (Department of Plant Science, Kangnung-Wonju National University) ;
  • Rin, Ernest James (Department of Plant Pathology and Microbiology, University of California) ;
  • Coffey, Michael D. (Department of Plant Pathology and Microbiology, University of California)
  • 발행 : 2009.06.30
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초록

본 연구는 P. capsici의 난포자를 다량으로 확보하기 위하여 우수한 액체 배지를 선발하고 난포자의 발아율을 조사할 뿐만 아니라 $F_1$ progeny를 확보하기 위하여 수행하였다. 공시한 8개 액체배지 중 carrot broth와 V8C broth가 난포자형성을 위해 가장 적당한 배지였다. 총 11균주의 P. capsici(CapA형)과 P. tropicalis(CapB형)를 선발하였고 P. capsici(CapA형)에 P. tropicalis(CapB형)을 교배하는 서로 다른 9개 조합의 교배에서 얻은 난포자로부터 총 129 균주의 $F_1$ hybrid progeny를 얻었으며 난포자의 발아율은 0.64-4.0%(평균 1.85%)이었다.

In this study, we selected suitable broth media for mass production of Phytophthora capsici oospore, investigated oospore germination and secured $F_1$ progeny. Carrot broth and V8C broth were determined most effective for oospore formation by calculating and comparing oospore concentration produced from 8 different liquid media. Eleven strains were selected from P. capsici (CapA)/P. tropicalis (CapB) and 9 crosses were formed. Oospore progeny were produced, isolated and germinated from A1 and A2 combinations of P. capsici (CapA) with P tropicalis (CapB). This resulted in a total number of 129 $F_1$ isolates of P. capsici/P. tropicalis with a 0.64-4.0% (mean 1.85%) oospore germination.

키워드

참고문헌

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