Membrane Journal (멤브레인)

The Membrane Society of Korea (한국막학회)
권호 표지

Removal of Salts from Hydrolyzed Protein Solution by Membrane

분리막을 이용한 단백질 가수분해 용액의 탈염

  • Kim, In-Chul (Advanced Chemical Technology Division, Korea Research Institute of Chemical Technology) ;
  • Kwon, Ja-Young (Advanced Chemical Technology Division, Korea Research Institute of Chemical Technology) ;
  • Park, Joo-Young (Advanced Chemical Technology Division, Korea Research Institute of Chemical Technology) ;
  • Jeong, Bo-Reum (Advanced Chemical Technology Division, Korea Research Institute of Chemical Technology) ;
  • Lee, Ki-Hoon (Department of Biosystems Engineering, Seoul National University)
  • 김인철 (한국화학연구원 신화학연구단 환경에너지연구센터) ;
  • 권자영 (한국화학연구원 신화학연구단 환경에너지연구센터) ;
  • 박주영 (한국화학연구원 신화학연구단 환경에너지연구센터) ;
  • 정보름 (한국화학연구원 신화학연구단 환경에너지연구센터) ;
  • 이기훈 (서울대학교 바이오시스템공학부)
  • Published : 2009.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

The aim of this paper is to investigate removal of salts in hydrolyzed sericin solution by a prepared nanofiltration membrane. The nanofiltration membrane was prepared by the interfacial polymerization method, and then compared with commercialized nanofiltration membrane in terms of salts removal. The sericin solution was hydrolyzed by a protease. The molecular weight distribution of the hydrolyzed sericin was determined by electrophoresis method. Relative flux measurements were carried out to investigate membrane fouling.

본 연구의 목적은 나노복합막을 제조하여 가수분해된 세리신 용액에 함유되어 있는 염의 제거 거동을 살펴보는 것이다. 계면중합법으로 나노복합막을 제조하고 상용화되어 있는 나노복합막과 탈염 거동을 비교하였다. 세리신 용액은 단백질 분해효소에 의해서 분해하여 저분자화하였고 전기영동에 의해 분자량 분포를 살펴보았다. 상대투과유량을 조사하여 나노복합막의 막오염 정도를 살펴보았다. 기공크기가 큰 나노복합막이 탈염에 효율적인 것을 확인하였다.

Keywords

References

  1. C. Fabiani, M. Pissichini, M. Spadoni, and G. Zeddita, 'Treatment of waste water from silk degumming processes for protein recovery and water reuse', Desalination, 105, 1 (1995) https://doi.org/10.1016/0011-9164(96)00050-1
  2. J. H. Wu, Z. Wang, and S. Y. Xu, 'Preparation and characterization of serici powder extracted from silk industry wastewater', Food Chem., 103, 1255 (2007) https://doi.org/10.1016/j.foodchem.2006.10.042
  3. G. H. Altman, F. Diaz, C. Jakuba, T. Calabro, R. L. Horan, J. Chen, H. Lu, J. Richmond. and D. L. Kaplan, 'Silk-based bionaterals', Biomaterials, 24, 401 (2003) https://doi.org/10.1016/S0142-9612(02)00353-8
  4. R. B. Bhatia and C. J. Brinker, 'Aqueous Sol-Gel process for protein encapsulation', Chem. Mater., 12, 2434 (2000) https://doi.org/10.1021/cm000260f
  5. L. Copolovici and U. Niinemets, 'Salting-in and salting-out effects of ionic and neutral osmotica on limonene and linalool Henry's law contents and octanol/water partition coefficients', Chemoshere， 69, 621 (2007) https://doi.org/10.1016/j.chemosphere.2007.02.066
  6. M. Alaee, R. Whital, and M. Strachan, 'The effect of water temperature and composition on Henry's law constant for various PAH's', Chemosphere, 32, 1153 (1996) https://doi.org/10.1016/0045-6535(96)00031-8
  7. J. Art, M. Chelkowska, K. Kasiura, and P. Pietrzykowski， 'The fatty acids as penetration enhancers of amino acids by ion pairing', In. J. Cosmetic. Sci., 24, 313 (2002) https://doi.org/10.1046/j.1467-2494.2002.00155.x
  8. Y. Lee, 'Silk Reeling and Sericulture', FAO Agricultural Sevices Bullein, 136, 1 (2005)
  9. J. Y. Jang, Y. S. Chung, Y. M. Lee, and S. Y. Nam, 'Preparation and Properties of Membranes for the Application of Desalting, Refining and Concentration for Dye Processing', Membrane Journal, 16, 213 (2006)
  10. D. H. Son, Y. H. Lee, J. K. Koo, N. J. Cho, and D. I. Jang, 'Effect of Electrical Field on Ultrafiltration for Protein Separation', Membrane Journal, 15, 132 (2005)
  11. D. H. Son, H. J. Jun, Y. H. Lee, J. K. Koo, N. J. Cho, and D. L. Jang, 'Separation and Purification of Lysozyme from Chicken Eggwhite Through Ultrafiltration', Membrane Journal, 15, 121 (2005)
  12. I. C. Kim, K. H. Lee， J. Y. Park, B. R. Jeong, J. Y. Kwon, and K. H. Lee, 'Separation of Protein from Degumming Solution By Ultrafiltration Membrane'， Membrane Journal, 17, 375 (2007)
  13. J. Wang, D. Qu, M. Tie, H. Ren, X. Peng, and Z. Luan, 'Effect of coagulation pretreatment on membrane distillation process for desalination of recirculating cooling water', Separation and Purification Technology, 64, 108 (2008) https://doi.org/10.1016/j.seppur.2008.07.022
  14. J. Belal, D. Abutarboush, T. Matsuura, H. A. Arafat, and R. M. Narbaitz, 'Preparation of thin-film-composite polyamide membranes for desalination using novel hydrophilic surface modifying macromolecules', Journal of Membrane Science, 325, 166 (2008) https://doi.org/10.1016/j.memsci.2008.07.037
  15. A. S. Jonsson, A. K. Nordin, and O. Wallberg, 'Concentration and purification of lignin in hardwood kraft pulping liquor by ultrafiltration and nanofiltration', Chemical Engineering Research and Design， 86, 1271 (2008) https://doi.org/10.1016/j.cherd.2008.06.003
  16. I. C. Kim, I. H. Choi, B. R. Min, and K. H. Lee, 'Investigation of photocatalytic process on removal of natural organic matter in nanofiltration process', Membrane Journal, 17, 375 (2007). Membrane Journal, 17, 375 (2007)