Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Effects of Radix Aconiti Herbal Acupuncture Injected at Hwando($GB_{30}$) on Neuropathic Pain in Rats

환도(環跳)($GB_{30}$) 초오약침(草烏藥鍼)이 L5 척수신경 결찰에 의한 흰쥐의 동통모델에 미치는 영향

  • Bang, Sung-Pil (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Ryu, Mi-Seon (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Kim, Jae-Hong (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Wei, Tung-Sheun (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Yun, Dae-Hwan (Department of Acupoint, College of Oriental Medicine, Dongshin University) ;
  • Yun, Yeo-Choong (Department of Acupuncture and Moxibustion, College of Oriental Medicine, Dongshin University) ;
  • Yeo, Kyeong-Chan (Department of Acupuncture and Moxibustion, National Medical Center) ;
  • Jung, Ho-Seok (Department of Acupuncture and Moxibustion, Jaseng Hospital of Oriental Medicine)
  • 방성필 (동신대학교 한의과대학 침구학교실) ;
  • 류미선 (동신대학교 한의과대학 침구학교실) ;
  • 김재홍 (동신대학교 한의과대학 침구학교실) ;
  • 위통순 (동신대학교 한의과대학 침구학교실) ;
  • 윤대환 (동신대학교 한의과대학 경혈학교실) ;
  • 윤여충 (동신대학교 한의과대학 침구학교실) ;
  • 여경찬 (국립의료원 침구과) ;
  • 정호석 (자생한방병원 침구과)
  • Received : 2009.11.04
  • Accepted : 2009.11.17
  • Published : 2009.12.20
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Abstract

Objectives : The purpose of this study is to examine if Radix Aconiti herbal acupuncture may be effective to the neuropathic pain(mechanical allodynia, cold allodynia) in a rat model of neuropathic pain. Methods : To produce the model of neuropathic pain, under isoflurane 2.5% anesthesia, L5 spinal nerve was ligated by 6-0 silk thread. After neurophathic surgery, the author examined if the animals exhibited the behavioral sign of allodynia. The allodynia was assessed by stimulating the medial malleolus with von frey filament and acetone. Three days after the neuropathic surgery, Radix Aconiti herbal acupuncture was injected at Hwando($GB_{30}$) one time per two days for 2 weeks. After that, the author examined the withdrawal response of neuropathic rats' legs by von frey filament and acetone stimulation. And also the author examined c-Fos in the midbrain central gray of neuropathic rats and the change of WBC, RBC, HGB, HCT count in the blood of neuropathic rats. Results : The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) decreased the withdrawal response of mechanical allodynia that assessed with von frey filament in RA1-$GB_{30}$, RA2-$GB_{30}$ group as compared with control group. The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) decreased the withdrawal response of chemical allodynia(cold allodynia) that assessed with acetone in RA1-$GB_{30}$, RA2-$GB_{30}$ group as compared with control group. The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) showed the significant between control group and RA2-$GB_{30}$ group in the c-Fos expression. The Radix Aconiti herbal acupuncture injected Hwando($GB_{30}$) did not show the significant between control group and RA1-$GB_{30}$ group, RA2-$GB_{30}$ group and RA3-$GB_{30}$ group in the WBC, RBC, HGB, HCT count. Conclusions : We have noticed that Radix Aconiti herbal acupuncture at Hwando($GB_{30}$) decreased mechanical allodynia and cold allodynia in the model of neuropathic pain compared with the control group. C-fos expression in the central gray of that group was also decreased compared with the control group. This study can be used as a basic resource on a study and a treatment of pain.

Keywords

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