The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
권호 표지

Pathways Analysis of Gleditsia spina Extract on Changes of Gene Expression in Human Melanoma cells

조각자(皂角刺)가 악성흑색종 세포주에 미치는 영향

  • Received : 2009.08.14
  • Accepted : 2009.11.30
  • Published : 2009.12.25
Download PDF
⟨ Previous Next ⟩

Abstract

Glenditsia spina (GS) can resolve carbuncle, relive swelling, dispel wind and destroy parasites. For these reasons, GS has been widely used as dermatologic agent clinically. In this study, the specific pathways of anti-proliferative effect of GS on human derived melanoma cells were identified. The molecular profile was measured using microarray technique to identify up- or down-regulated genes in SK-MEL-2 cell line. Pathway analysis was done by listing percentage of pathway involvement, and the represented pathways were obtained from KEGG. The transcription factor binding sequences were obtained by Transfac database. By the promoter analysis, up-regulated genes by GS were mainly associated with MAPK, Regulation of actin cytoskeleton, Wnt, Focal adhesion and Long term potentiation pathway. Down-regulated genes by GS were mainly associated with MAPK and Antigen processing and presentation pathway. And some of the transcription factors like Sp1 and NF-Y in up-regulated genes and Oct-1 in down-regulated genes by GS also identified.

Keywords

References

  1. Iizuka N, Oka M, Yamada-Okabe H, Mori N, Tamesa T, Okada T. Takemoto N, Tangoku A, Hamada K, Nakayama H, Miyamoto T, Uchimura S, and Hamamoto Y. Comparison of gene expression profiles between hepatitis B virus- and hepatitis C virus-infected hepatocellular carcinoma by oligonucleotide microarray data on the basis of a supervised learning method. Cancer Res. 2002;62:3939-44.
  2. Hong Y, Ho KS, Eu KW and Cheah PY. A susceptibility gene set for early onset colorectal cancer that integrates diverse signaling pathways: implication for tumorigenesis. Clin Cancer Res. 2007;13(4):1107-14. https://doi.org/10.1158/1078-0432.CCR-06-1633
  3. Galbraith DW. The daunting process of MIAME. Nature. 2006;444(7115):31.
  4. Blanchard AP, Kaiser RJ and Hood LE. High-density oligonucleotide arrays. Biosensors Bioelectron. 1996;11:687-690. https://doi.org/10.1016/0956-5663(96)83302-1
  5. Hughes TR, Mao M, Jones AR, Burchard J, Marton MJ, Shannon KW, Lefkowitz SM, Ziman M, Schelter JM, Meyer MR, et al. Expression profiling using microarrays fabricated by an ink-jet oligonucleotide synthesizer. Nat Biotechnol. 2001;19:342-347. https://doi.org/10.1038/86730
  6. Iizuka N, Oka M, Yamada-Okabe H, Nishida M, Maeda Y, Mori N, Takao T, Tamesa T, Tangoku A, Tabuchi H, Hamada K, Nakayama H, Ishitsuka H, Miyamoto T, Hirabayashi A, Uchimura S and Hamamoto Y. Oligonucleotide microarray for prediction of early intrahepatic recurrence of hepatocellular carcinoma after curative resection. Lancet. 2003;361:923-929. https://doi.org/10.1016/S0140-6736(03)12775-4
  7. 전국한의과대학 본초학교수 공편저. 本草學. 서울:永林社. 1998:440.
  8. 신민교. 임상본초학. 서울:永林社. 2000:668-9.
  9. 江蘇新醫學院. 中藥大辭典(上). 서울:成輔社. 1980:1144-7.
  10. 許浚. 東醫寶鑑. 서울:法人文化社. 1999:1984.
  11. 尹吉榮. 東醫臨床方劑學. 서울:明寶出版社. 1985:83.
  12. 金仁洛. 皂莢과 조각자의 기원에 대한 연구. 大韓本草學會誌. 2006;21(1):44-9.
  13. 박은희, 신미자. 조각자 물추출물의 항염증 작용. 약학회지. 1993;37(2):124-8.
  14. 권두한, 김만배, 윤도영, 이영희, 김재화, 이희구, 회인성, 임종석, 최용경. 항바이러스 활성 식물자원 탐색. 한국약용작물학회지. 2003;11(1):24-30.
  15. 朴涌基, 康秉秀, 安相炫, 朴仁植, 金鎭澤. 皂角刺의 抗癌作用에 대한 實驗的 硏究. 대한본초학회지. 1997;12(1):53-66.
  16. Florell SR, Coffin CM, Holden JA, Zimmermann JW, Gerwels JW, Summers BK, Jones DA and Leachman SA. Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol. Mod Pathol. 2001;14(2):116-28. https://doi.org/10.1038/modpathol.3880267
  17. Weis S, Llenos IC, Dulay JR, Elashoff M, Martinez-Murillo F and Miller CL. Quality control for microarray analysis of human brain samples: The impact of postmortem factors, RNA characteristics, and histopathology. J Neurosci Methods. 2007;165(2):198-209. https://doi.org/10.1016/j.jneumeth.2007.06.001
  18. Kwon KH, Kim SJ, Kim HJ and Jung HH. Analysis of gene expression profiles in cholesteatoma using oligonucleotide microarray. Acta Otolaryngol. 2006;126(7):691-7. https://doi.org/10.1080/00016480500475633
  19. Zahurak M, Parmigiani G, Yu W, Scharpf RB, Berman D, Schaeffer E, Shabbeer S and Cope L. Pre-processing Agilent microarray data. BMC Bioinformatics. 2007;8:142. https://doi.org/10.1186/1471-2105-8-142
  20. Pavlcek A, Hrda S and Flegr J. Free-Tree--freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of genus Frenkelia. Folia Biol (Praha). 1999;45(3):97-9.
  21. 李時珍. 本草綱目 3券. 서울:醫聖堂. 1993:2021-2.
  22. 朴涌基. 조각자나무의 부위 및 채취시기별 Saponin 함량차이에 관한 연구. 동국대학교 대학원. 1990.
  23. 김충희, 하대식, 류재두, 허정호, 정명호, 최영태, 김곤섭, 김종수. 랫드에서 조각자(주엽) 나무 추출물인 gleditschia saponin의 경구 2주 반복투여 독성실험. 한국독성학회. 2002;18(3):285-92.
  24. 송상진. 조각자가 천식모델 생쥐의 면역세포 및 사이토카인에 미치는 영향. 대전대학교 대학원. 2005.
  25. 문구, 정병학, 김병주. 암 동서의 결합치료. 원광대학교출판국:신아출판사. 1999:93-7.
  26. Choi HJ, Yee SB, Park SE, Im E, Jung JH, Chung HY, Choi YH and Kim ND. Petrotetrayndiol A induces cell cycle arrest and apoptosis in SK-MEL-2 human melanoma cells through cytochrome c-mediated activation of caspases. Cancer Lett. 2006;232(2):214-25. https://doi.org/10.1016/j.canlet.2005.02.030
  27. 박경덕, 이석종, 이원주, 김도원, 정호윤, 조병채. 피부 악성 흑색종의 임상 및 병리조직학적 소견. 대한피부과학회지. 2007;45(2):149-58.
  28. 조용찬, 김완, 조은택, 서영호, 황선호, 기영화, 이봉규, 강원유, 정다운, 김삼철, 고향미, 정성광. 불명확한 원발부위의 전이성 대장 악성흑색종 1예. 대한소화기내시경학회지. 2006;32:62-6
  29. 한진근. 楡白皮 추출물이 인간의 피부 세포 재생 및 악성 흑색종 세포에 미치는 영향. 동신대학교 박사학위논문. 2007.
  30. 고홍개. 化氣調經湯이 피부 세포 재생 및 악성 흑색종 세포에 미치는 영향. 동신대학교 박사학위논문 2007.