Journal of Korean Academy of Nursing (대한간호학회지)

Korean Society of Nursing Science (한국간호과학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of DHEA Administration Alone or Exercise combined with DHEA before Steroid Treatment on Rat Hindlimb Muscles

스테로이드 치료 전 DHEA 단독투여와 DHEA 투여와 운동의 동시적용이 스테로이드에 의해 유발되는 쥐 뒷다리근의 위축 예방에 미치는 효과

  • 최명애 (서울대학교 간호대학) ;
  • 안경주 (대구가톨릭대학교 간호학과)
  • Published : 2009.06.26
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: The purpose of this study was to determine the effect of Dehydroepiandrosterone(DHEA) administration alone or exercise combined with DHEA before steroid treatment on rat hindlimb muscles. Methods: Male Sprague-Dawley rats were assigned to one of three groups: a steroid group(S, n=10) that had no treatment for 7 days before steroid treatment; a DHEA-steroid group(DS, n=8) that had 0.34 mmol/kg/day DHEA injection once a day for 7 days before steroid treatment and an exercise+DHEA-steroid group(EDS, n=9) that ran on the treadmill combined with 0.34 mmol/kg/day DHEA injection for 7 days before steroid treatment. At 15 days all rats were anesthetized and soleus, plantaris and gastrocnemius muscles were dissected. Body weight, food intake, muscle weight, myofibillar protein content and cross-sectional area of the dissected muscles were determined. Results: The DS group showed significant increases(p<.05) as compared to the steroid group in body weight, and muscle weight of gastrocnemius muscles. The EDS group showed significant increases(p<.05) as compared to the S group in body weight, muscle weight, myofibrillar protein content, and Type II fiber cross-sectional area of soleus, plantaris and gastrocnemius muscles. Conclusion: Exercise combined with DHEA administration before steroid treatment prevents steroid induced muscle atrophy, with exercise combined with DHEA administration being more effective than DHEA administration alone in preventing muscle atrophy.

Keywords

References

  1. An, G. J., Lee, Y. K., Lim, J. H., Choi, S., & Choe, M. A. (2000). Effect of exercise during acute stage on hindlimb muscles of stroke induced rat. Journal of Korean Biological Nursing Science, 2(2), 67-80
  2. An, G. J., & Choe, M. A. (2002). Effect of DHEA on type I and II muscles in a focal cerebral ischemia model rat. Journal of Korean Biological Nursing Science, 4(2), 19-42
  3. Apostolova, G., Schweizer, R. A., Balazs, Z., Kostadinova, R. M., & Odermatt, A. (2005). Dehydroepiandrosterone inhibits the amplication of glucocorticoid action in adipose tissue. American Journal of Physiology Endocrinology and Metabolism, 288, E957-964 https://doi.org/10.1152/ajpendo.00442.2004
  4. Araneo, B., & Daynes, R. (1995). DHEA functions as more than antiglucocorticoid in preserving immunocompetence after thermal injury. Endocrinology, 136, 393-401 https://doi.org/10.1210/en.136.2.393
  5. Blundell, J. E., & King, N. A. (1999). Physical activity and regulation of food intake: Current evidence. Medical Science of Sports &Exercise, 31(11 Suppl), 573-583
  6. Brown, G. A., Vukovich, M. D., Sharp, R. L., Reifenrath, T. A., Parsons, K. A., & King, D. S. (1999). Effect of oral DHEA on serum testosterone and adaptations to resistance training in young men. Journal of Applied Physiology, 87, 2274-2283
  7. Choe, M. A., Choi, J. A., & Shin, G. S. (1997). Effect of regular exercise during dexamethasone on the body weight, weight of hindlimb muscle and adrenal gland in young rats. Journal of Korean Academy of Nursing, 27, 510-519
  8. Choe, M. A. (1998). Effect of endurance exercise during dexamethasone treatment on the attenuation of atrophied hindlimb muscle induced by dexamethasone in rats. Journal of Korean Academy of Nursing, 28, 893-907
  9. Choe, M. A., Shin, G. S., Lee, E. J., & An, G. J. (2001). Effect of DHEA administration during dexamethasone treatment on mass of hindlimb muscles of rat. Journal of Korean Biological Nursing Science, 3, 63-74
  10. Choe, M. A., Shin, G. S., An, G. J., Choi, J. A., & Lee, Y. K. (2002). Effect of regular exercise during recovery period following steroid treatment on the atrophied type II muscles induced by steroid in young rats. Journal of Korean Academy of Nursing, 32, 550-559
  11. Choe, M. A., & An, G. J. (2007). Effects of exercise before steroid treatment on type I and type II hindlimb muscles in a rat model. Journal of Korean Academy of Nursing, 37, 81-90
  12. Dhatariya, K., Bigelow, M. L., & Nair, K. S. (2005). Effect of dehydroepiandrosterone replacement on insulinsensitivity and lipids in hypoadrenal women. Diabetes, 54, 765-769 https://doi.org/10.2337/diabetes.54.3.765
  13. Goldspink, G. (2005). Mechanical signals, IGF-I gene splicing, and muscle adaptation. Physiology, 20, 232-238 https://doi.org/10.1152/physiol.00004.2005
  14. Horber, F. F., Hoppeler, H., Scheidegger, J. R., Grunig, B. E., & Frey, F. J. (1987). Impact of physical-training on the ultrastructure of midthigh muscle in normal subjects and in patients treated with glucocorticoids. The Journal of Clinical Investigation, 79, 1181-1190 https://doi.org/10.1172/JCI112935
  15. Kim, Y. S., & Kim, C. G. (1991). Muscle fiber types by ATPase staining method. The Research Institute of Physical Education & Sports Science, 10, 93-103
  16. Kostka, T., Arsac, L. M., Patricot, M. C., Berthouze, S. E., Lacour, J. R., & Bonnefoy, M. (2000). Leg extensor power and DHEA sulfate, insulin-like growth factor-I and testosterone in healthy active elderly people. European Journal of Applied Physiology, 82, 83-90 https://doi.org/10.1007/s004210050655
  17. Kwak, C. K., Kang, C. M., Kang, H. S., Song, K. Y., Lee, M. S., Song, S. C., et al. (2000). Dehydroepiandrosterone-dependent induction of peroxisomal proliferation can be reduced by aspartyl esterification animal model. Journal of Korean Medical Science, 15, 533-541
  18. Lapier, T. K. (1997). Glucocorticoid-induced muscle atrophy: The role of exercise in treatment and prevention. Journal of Cardiopulmonary Rehabilitation, 17(2), 76-84 https://doi.org/10.1097/00008483-199703000-00002
  19. Lee, S. J., & McPherron, A. C. (1999). Myostatin and the control of skeletal muscle mass. Current Opinion Genetic Development, 9, 604-607 https://doi.org/10.1016/S0959-437X(99)00004-0
  20. Ma, K., Mallidis, C., Bhasin, S., Mahabadi, V., Artaza, J., Gonzalez-Cadavid, N., et al. (2003). Glucocorticoid-induced skeletal muscle atrophy is associated with upregulation of myostatin gene expression. American Journal of Physiolology Endocrinology &Metabolism, 285, E363-E371
  21. Morales, A. J., Haubrich, R. H., Hwang, J. Y., Asakura, H., & Yen, S. S. (1998). The effect of six months treatment with a 100mg daily dose of DHEA on circulating sex steroids, body composition and muscle strength in age-advanced men and women. Journal of Clinical Endocrinology &Metabolism, 78, 1360-1367 https://doi.org/10.1210/jc.78.6.1360
  22. Park, S. J., Lee, M. C., & Kim, S. J. (1999). Effect of physical exercise on experimental steroid-induced myopathy. Journal of Korean Neural Association, 17, 694-701
  23. Roubenoff, R. (2003). Sarcopenia: Effects on body composition and function. Journal of Gerontological Biology Science, 58, 1012-1017 https://doi.org/10.1093/gerona/58.11.M1012
  24. Sugino, M., Ohsawa, N., Ito, T., Ishida, S., Yamasaki, H., Kimura, F., et al. (1998). A pilot study of dehydroepi-androsterone sulfate in myotonic dystrophy. Neurology, 51, 586-598 https://doi.org/10.1212/WNL.51.2.586
  25. Tsuji, K., Furutama, D., Tagami, M., & Ohsawa, N. (1999). Specific binding and effects of DHEA-S on skeletal muscle cells. Life Science, 65, 17-26 https://doi.org/10.1016/S0024-3205(99)00215-5
  26. Villareal, D. T., Holloszy, J. O., & Kohrt, W. M. (2000). Effects of DHEA replacement on bone mineral density and body composition in elderly women and men. Clinical Endocrinology, 53, 561-568 https://doi.org/10.1046/j.1365-2265.2000.01131.x
  27. Villareal, D. T., & Holloszy, J. O. (2006). DHEA enhances effects of weight training on muscle mass and strength in elderly women and men. American Journal of Endocrinology &Metabolism, 291, E1003-1008 https://doi.org/10.1152/ajpendo.00100.2006

Cited by

  1. Effect of DHEA on Recovery of Muscle Atrophy Induced by Parkinson' s Disease vol.41, pp.6, 2011, https://doi.org/10.4040/jkan.2011.41.6.834
  2. 동물 실험을 적용한 국내 간호 연구 동향 분석 vol.17, pp.4, 2009, https://doi.org/10.7586/jkbns.2015.17.4.324