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Expression Pattern of Immunoproteasome Subunits in Human Thymus

  • Oh, Kwon-Ik (Department of Pathology, Hallym University College of Medicine) ;
  • Seo, Jae-Nam (Department of Pathology, Hallym University College of Medicine)
  • Received : 2009.12.01
  • Accepted : 2009.12.10
  • Published : 2009.12.31
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Abstract

The expression pattern of immunoproteasomes in human thymus has not been analyzed but may have important consequences during thymic selection. Here we examined the expression patterns of immunoproteasome subunits in fetal and adult thymic tissues by immunohistochemistry and found that all three subunits are expressed in both cortical and medullary stromal cells. These data suggest that thymic selection in human can be affected by peptide repertoires generated by immunoproteasomes.

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References

  1. Starr TK, Jameson SC, Hogquist KA: Positive and negative selection of T cells. Annu Rev Immunol 21;139-176, 2003 https://doi.org/10.1146/annurev.immunol.21.120601.141107
  2. Siggs OM, Makaroff LE, Liston A: The why and how of thymocyte negative selection. Curr Opin Immunol 18;175-183, 2006 https://doi.org/10.1016/j.coi.2006.01.001
  3. Cosgrove D, Chan SH, Waltzinger C, Benoist C, Mathis D: The thymic compartment responsible for positive selection of CD4+ T cells. Int Immunol 4;707-710, 1992 https://doi.org/10.1093/intimm/4.6.707
  4. Anderson G, Jenkinson EJ: Lymphostromal interactions in thymic development and function. Nat Rev Immunol 1; 31-40, 2001 https://doi.org/10.1038/35095500
  5. Kloetzel PM, Ossendorp F: Proteasome and peptidase function in MHC-class-I-mediated antigen presentation. Curr Opin Immunol 16;76-81, 2004 https://doi.org/10.1016/j.coi.2003.11.004
  6. Fruh K, Gossen M, Wang K, Bujard H, Peterson PA, Yang Y: Displacement of housekeeping proteasome subunits by MHC-encoded LMPs: a newly discovered mechanism for modulating the multicatalytic proteinase complex. Embo J 13;3236-3244, 1994
  7. Gaczynska M, Rock KL, Spies T, Goldberg AL: Peptidase activities of proteasomes are differentially regulated by the major histocompatibility complex-encoded genes for LMP2 and LMP7. Proc Natl Acad Sci U S A 91;9213-9217, 1994 https://doi.org/10.1073/pnas.91.20.9213
  8. Tanaka K, Kasahara M: The MHC class I ligand-generating system: roles of immunoproteasomes and the interferon-gamma-inducible proteasome activator PA28. Immunol Rev 163;161-176, 1998 https://doi.org/10.1111/j.1600-065X.1998.tb01195.x
  9. Nil A, Firat E, Sobek V, Eichmann K, Niedermann G: Expression of housekeeping and immunoproteasome subunit genes is differentially regulated in positively and negatively selecting thymic stroma subsets. Eur J Immunol 34;2681-2689, 2004 https://doi.org/10.1002/eji.200425032
  10. Chen W, Norbury CC, Cho Y, Yewdell JW, Bennink JR: Immunoproteasomes shape immunodominance hierarchies of antiviral CD8(+) T cells at the levels of T cell repertoire and presentation of viral antigens. J Exp Med 193;1319-1326, 2001 https://doi.org/10.1084/jem.193.11.1319
  11. Toes RE, Nussbaum AK, Degermann S, Schirle M, Emmerich NP, Kraft M, Laplace C, Zwinderman A, Dick TP, Muller J, Schonfisch B, Schmid C, Fehling HJ, Stevanovic S, Rammensee HG, Schild H: Discrete cleavage motifs of constitutive and immunoproteasomes revealed by quantitative analysis of cleavage products. J Exp Med 194;1-12, 2001 https://doi.org/10.1084/jem.194.1.1
  12. Bandoh N, Ogino T, Cho HS, Hur SY, Shen J, Wang X, Kato S, Miyokawa N, Harabuchi Y, Ferrone S: Development and characterization of human constitutive proteasome and immunoproteasome subunit-specific monoclonal antibodies. Tissue Antigens 66;185-194, 2005 https://doi.org/10.1111/j.1399-0039.2005.00462.x
  13. Niedermann G: Immunological functions of the proteasome. Curr Top Microbiol Immunol 268;91-136, 2002
  14. Jameson SC, Hogquist KA, Bevan MJ: Positive selection of thymocytes. Annu Rev Immunol 13;93-126, 1995 https://doi.org/10.1146/annurev.iy.13.040195.000521
  15. Barton GM, Rudensky AY: Requirement for diverse, low-abundance peptides in positive selection of T cells. Science 283;67-70, 1999 https://doi.org/10.1126/science.283.5398.67

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