The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Molecular Analysis of Korean Isolate of Barley mild mosaic virus (Iks Isolate)

  • Choi, Min-Kyung (Division of Agri-Food and Horticulture, Jeollabuk-Do Agricultural Research and Extension Services) ;
  • Kamala-Kannan, Seralathan (Division of Biotechnology, Chonbuk National University) ;
  • Oh, Byung-Taek (Division of Biotechnology, Chonbuk National University) ;
  • Park, Jong-Chul (Honam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Gun-Woong (Division of Biotechnology, Chonbuk National University) ;
  • Lee, Kui-Jae (Division of Biotechnology, Chonbuk National University) ;
  • Park, Yool-Jin (Department of Environmental Landscape Architecture-Design, Chonbuk National University)
  • 발행 : 2009.06.30
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초록

The complete nucleotide sequences of both RNA of an isolated Barley mild mosaic virus (BaMMV) from Iksan, Korea, have been determined. RNA1 was 7273 nucleotides long and encodes for a polyprotein of 2261 amino acids, which contains the eight putative functional proteins. RNA2 was 3520 nucleotides long and encodes for a polyprotein of 894 amino acids, which contains two functional proteins. Results of multiple sequence alignment showed 92.9% similarity with Na1 isolate, followed by Sil, UK(F), Asl1, Remis M and UK(M) isolates, respectively. Comparison of the BaMMV-Iks polyproteins with the corresponding proteins of BaMMV-Na1 isolates showed 95% amino acid sequence identity. The phylogenetic analysis revealed that Iks isolate was closely related to Na1 strain and have a common origin.

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참고문헌

  1. Dessens, J. and Meyer, M. 1995. Characterization of fungally and mechanically transmitted isolates of Barley mild mosaic virus: two strains in competition. Virology 212:383-391 https://doi.org/10.1006/viro.1995.1495
  2. Dougherty, W J. and Parks, T D. 1991. Post-translational processing of the tobacco etch virus 49 kDa smaller nuclear inclusion polyprotein: identification of internal cleavage site and delimitation of VPg and proteinase domain. Virology 183 :449-456 https://doi.org/10.1016/0042-6822(91)90974-G
  3. Gallie, D. R., Sleat, D. E., Watts, J. W, Turner, P. C. and Wilson, T. M. A. 1987. A comparison of eukaryotic viral 5'-leader sequences as enhancers of mRNA expression in vivo. Nucleic Acids Res. 15:8693-5711 https://doi.org/10.1093/nar/15.21.8693
  4. Hariri, D., Meyer, M. and Prudhomme, H. 2003. Characterization of a new Barley mild mosaic virus pathotype in France. Eur. J. Plant Pathol. 10:921-928 https://doi.org/10.1023/B:EJPP.0000003663.32298.f4
  5. Kamer, G and Argos, P. 1984. Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial virus RNA. Nucleic Acids Res. 12:7269-7282 https://doi.org/10.1093/nar/12.18.7269
  6. Kashiwazaki, S., Hayano, Y., Minobe, Y., Omura, T., Hibino, H. and Tsuchizaki, T. 1989. Nucleotide sequence of the capsid protein gene of Barley yellow mosaic virus. J. Gen. Virol. 70:3015-3023 https://doi.org/10.1099/0022-1317-70-11-3015
  7. Kashiwazaki, S., Minobe, Y., Omura, T. and Hibino, H. 1990. Nucleotide sequence of Barley yellow mosaic virus RNA1: a close evolutionary relationship with potyvirus. J. Gen. Virol. 71:2781-2790 https://doi.org/10.1099/0022-1317-71-12-2781
  8. Kashiwazaki, S. 1996. The complete nucleotide sequence and genome organization of Barley mild mosaic virus (Nal strain). Arch. Virol. 141:2077-2089 https://doi.org/10.1007/BF01718216
  9. Jonson, G, Park J. H., Noh, T H., Kim, M. J., Hyun, J. N. and Kim, J. G 2006. Isolation and biological characterization of Barley mild mosaic virus (BaMMY) mild and severe strains in Korea. Plant Pathol. J. 22:329-333 https://doi.org/10.5423/PPJ.2006.22.4.329
  10. Lee, K. J., Choi, M. K., Lee, H. W. and Rajkumar, M. 2006. Molecular analysis of Korean isolate of Barley yellow mosaic virus. Virus Genes 32: 171-176 https://doi.org/10.1007/s11262-005-6873-5
  11. Peerenboom, E., Jacobi, V, Antoniw, J. F., Schlichter, U., Cartwright, J. E., Steinbis, H. H. and Adams, J. M. 1996. The complete nucleotide sequence of RNA-2 of a fungally-transmitted UK isolate of barley mild mosaic bymovirus and identification of amino acid combinations possibly involved in fungus transmission. Virus Res. 40:149-159 https://doi.org/10.1016/0168-1702(95)01267-2
  12. Peerenboom, E., Cartwright, E. J., Foulds, I., Adams, M. J., Stratford, R., Rosner, A, Steinbiss, H. H. and Antoniw, J. F. 1997. Complete RNAI sequence of two UK isolates of Barley mild mosaic virus: a wild type fungus-transmissible isolate and a non-fungus-transmissible derivative. Virus Res. 50:175-183 https://doi.org/10.1016/S0168-1702(97)00069-5
  13. Shukla, D. D. and Ward, C. W. 1988. Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. J. Gen. Virol. 69:2703-2710 https://doi.org/10.1099/0022-1317-69-11-2703
  14. Shukla, D. D., Ward, C. Wand Brunt, A. A. 1994. The Potyviridae. CAB nternational, Wallingford. Timpe, U. and Kuhne, T. 1994. The complete nucleotide sequence of RNA2 of Barley mild mosaic virus (BaMMY). Eur. J. Plant. Pathol. 100:233-241 https://doi.org/10.1007/BF01876238
  15. Timpe, U. and Kuhne, T. 1995. In vitro transcripts of a full length cDNA of a naturally deleted RNA2 of Barley mild mosaic virus (BaMMY) replicate in BaMMY-infected plants. J. Gen. Virol. 76:2619-2623 https://doi.org/10.1099/0022-1317-76-10-2619
  16. Usugi, T. and Saito, Y. 1976. Purification and serological properties of Barley yellow mosaic virus and wheat yellow mosaic virus. Ann. Phytopathol. Soc. Japan 42: 12-20 https://doi.org/10.3186/jjphytopath.42.12
  17. Zheng, T., Cheng, Y., Chen, J. P., Antoniw, J. F. and Adams, M. J. 1999. The occurrence of Barley mild mosaic virus (BaMMY) in china and the nucleotide sequence of its coat protein gene. J. Phytopathol. 147:229-234 https://doi.org/10.1046/j.1439-0434.1999.147004229.x