YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effect of Licochalcone A on the Production of Cytokines in LPS-Activated RAW264.7 Macrophage Cells

Licochalcone A가 대식세포주의 사이토카인 생성에 미치는 영향

  • Lee, Gi-Sae (College of Pharmacy & Research Institute of Drug Development, Chonnam National University) ;
  • Lee, Sung-Ho (College of Pharmacy & Research Institute of Drug Development, Chonnam National University) ;
  • Cho, Young-Chang (College of Pharmacy & Research Institute of Drug Development, Chonnam National University) ;
  • Yoon, Goo (Korea Institute of Science and Technology, Gangneung Institute) ;
  • Cheon, Seung-Hoon (College of Pharmacy & Research Institute of Drug Development, Chonnam National University) ;
  • Kang, Bok-Yun (College of Pharmacy & Research Institute of Drug Development, Chonnam National University)
  • 이기세 (전남대학교 약학대학 & 약품개발연구소) ;
  • 이성호 (전남대학교 약학대학 & 약품개발연구소) ;
  • 조영창 (전남대학교 약학대학 & 약품개발연구소) ;
  • 윤구 (강릉 한국과학기술연구원) ;
  • 천승훈 (전남대학교 약학대학 & 약품개발연구소) ;
  • 강복윤 (전남대학교 약학대학 & 약품개발연구소)
  • Received : 2009.04.08
  • Accepted : 2009.12.27
  • Published : 2009.12.31
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Abstract

Licochalcone A is a chalcone isolated from the roots of Glycyrrhiza inflate. In this study, we examined the effects of licochalonce A on the production of cytokines in LPS-activated macrophages. Licochalcone A inhibited the secretion of proinflammatory cytokines such as IL-1$\beta$, IL-6, and TNF-$\alpha$. The reduced secretion of proinflammatory cytokines is related to the differences in the mRNA expression of IL-1$\beta$, IL-6, and TNF-$\alpha$. Moreover, licochalcone A inhibited the mRNA expression of IL-12p40, IL-18, and IL-23p19. To investigate its mechanism, we performed gel shift assay. Licochalcone A reduced nuclear NF-${\kappa}B$ binding activity in LPS-activated RAW264.7 cells. Taken together, these results suggest that licochalcone A has anti-inflammatory effects in LPS-activated macrophages and its mechanism could be through the down-regulation of binding to the ${\kappa}B$ site.

Keywords

References

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