한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제17권3호
  • /
  • Pages.210-216
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  • 2009
  • /
  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

홍경천의 면역활성이 증진된 용매별 분획 추출물

Improvement of Immune Activities of Rhodiola sachalinensis A. Bor. by Serial Solvent Fractionization

  • 하지혜 (강원대학교 BT특성화학부대학) ;
  • 김철희 (강원대학교 BT특성화학부대학) ;
  • 정향숙 (강원대학교 BT특성화학부대학) ;
  • 김영 (강원대학교 BT특성화학부대학) ;
  • 오성호 (강원대학교 BT특성화학부대학) ;
  • 김승섭 (강원대학교 BT특성화학부대학) ;
  • 정명훈 (강원대학교 BT특성화학부대학) ;
  • 마충제 (강원대학교 BT특성화학부대학) ;
  • 남종현 ((주)그래미) ;
  • 황백 (전남대학교 생물학과) ;
  • 최근표 (강원도립대학교 식품가공제과제빵과) ;
  • 박욱연 (강원도립대학교 식품가공제과제빵과) ;
  • 이현용 (강원대학교 BT특성화학부대학)
  • Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Kim, Cheol-Hee (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Jeong, Hyang-Suk (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Jin, Ling (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Oh, Sung-Ho (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Kim, Seung-Seop (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Jeong, Myoung-Hoon (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Ma, Choong-Je (College of Bioscience and Biotechnology, Kangwon National Univ.) ;
  • Nam, Jong-Hyun (Grami Co., Ltd.) ;
  • Hwang, Baik (Department of Biology, Chonnam National Univ.) ;
  • Choi, Geun-Pyo (Department of Food Processing & Bakery, Gangwon Provincial Univ.) ;
  • Park, Uk-Yeon (Department of Food Processing & Bakery, Gangwon Provincial Univ.) ;
  • Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National Univ.)
  • 발행 : 2009.06.30
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초록

This study was performed to compare the effects of immuno-modulating activities of Rhodiola sachalinensis A. Bor. fractionized by consecutive solvent separation. The Cytotoxicity of all fractionized extracts on human kidney cell (HEK293) was lower than crude extracts. Generally, the butanol and chloroform extracts showed less cytotoxicity on about 10.07% and 9.67% than the crude extracts. For human immune B and T cell growth, chloroform fraction showed the highest cell growth compared to the control. The secretion of cytokines (IL-6, $TNF-\alpha$) on human B and T cells were increased by adding chloroform extracts. Also, NK cell growth was significantly improved up to nearly 30% by adding the supernatant of B cell medium grown with the chloroform fraction. It was also found that chloroform fraction could yield higher nitric oxide production from macrophage than untreated control cells. Differentiation of HL-60 cells was increased up to 131.9% after treatment with chloroform fraction extracts, compared to the control. These results indicate that the chloroform fraction of R. sachalinensis have high immune activation activity than others fractions and the crude extracts, implying that this chloroform fractions could be used a new functional material.

키워드

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