대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

목단피(牧丹皮)가 손상된 성상신경세포의 CD81 및 GFAP의 발현에 미치는 영향

The Effect of the Moutan Radicis Cortex on Expression of CD81 and GFAP in Injured Astrocyte

  • 문성진 (원광대학교 한의과대학 내과학교실) ;
  • 성기문 (원광대학교 한의과대학 내과학교실) ;
  • 임진영 (원광대학교광주병원 재활의학과) ;
  • 송봉근 (원광대학교 한의과대학 내과학교실)
  • Moon, Sung-Jin (Dept. of Internal Medicine, Wonk-wang University Oriental Medical School) ;
  • Seon, Ki-Moon (Dept. of Internal Medicine, Wonk-wang University Oriental Medical School) ;
  • Lim, Jin-Young (Dept. of Rehabilitation, Wonk-wang University Medical Center) ;
  • Song, Bong-Keun (Dept. of Internal Medicine, Wonk-wang University Oriental Medical School)
  • 발행 : 2009.03.31
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초록

Object : In conditions of brain infarction, irreversible axon damage occurs in the central nerve system (CNS), because gliosis makes physical and mechanical barriers. If gliosis formation could be suppressed, irreversible axon damage would be reduced. This could mean that an injured CNS could be regenerated. CD81 and GFAP have close relationships to gliosis. The increase in glial cells at CNS injury gives rise to the expression of CD81 and GFAP. CD81 was postulated to play a central role in the process of CNS scar formation. Method : In this study, the author investigated the effect of the water extract of the Moutan Radicis Cortex on regulation of CD81 and GFAP expression in injured CNS cells. MTT assay was used to examine cell viability, while RT-PCR and ELISA methods were carried out to measure the expression of CD81 and GFAP in the astrocyte. Results : We observed that water extract of the Moutan Radicis Cortex increased cell viability under hypoxia induced by $CoCl_2$ and suppressed the expression of CD81 and GFAP up-regulated by hypoxia. Conclusion : These results suggest that the Moutan Redicis Cortex could promote neural regeneration as a consequence of protecting CNS cells from hypoxia and suppressing the reactive gliosis following CNS injury.

키워드

참고문헌

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