대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지

Splenocyte, BMMC, eosinophil을 통해 본 단삼(丹蔘)이 천식 관련 염증세포에 미치는 영향

Effects of Salviae miltiorrhizae on Inflammatory Cells Associated with Asthma via Splenocyte, BMMC, Eosinophil

  • 정승연 (경희대학교 한의과대학 폐계내과학 교실) ;
  • 김진주 (경희대학교 약학대학 한방생리학교실) ;
  • 정희재 (경희대학교 한의과대학 폐계내과학 교실) ;
  • 정승기 (경희대학교 한의과대학 폐계내과학 교실)
  • Jeong, Seung-Yeon (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung-hee University) ;
  • Kim, Jin-Ju (Dept. of Oriental Physiology, College of Pharmacy, Kyung-hee University) ;
  • Jung, Hee-Jae (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung-hee University) ;
  • Jung, Sung-Ki (Division of Allergy, Immune & Respiratory System, Dept. of Internal Medicine, College of Oriental Medicine, Kyung-hee University)
  • 발행 : 2009.03.31
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초록

Objectives : Asthma is a chronic inflammatory disorder of the airways by many cells such as mast cells, Th2 lymphocytes and eosinophile. The present study was aimed to evaluate the effects of Salviae miltiorrhizae (SM) on T cell cytokine production, mast cells. and eosinophils, Methods : We screened 13 herbs to find compounds with potential to control Th cytokine production. using concanavalin A (con A)-activated splenocyte cultures. Con A-activated $IFN-\gamma$ and IL-4 levels in supernatants of splenocyte cultures. Bone marrow derived mast cells (BMMC) were incubated with SM and then the expressions of membrane proteins of BMMC were analyzed by fluorescence activated cell sorter (FACS). BALB/c mice sensitized to ovalbumin (OVA) were challenged with aerosolized OVA for 6 weeks. During the last weeks some mice were treated with SM. Then eosinophils in bronchoalveolar lavage fluid (BALf) were counted and pathologic changes of lung tissue were observed with hematoxylin-eosin stain. Results : SM increased $IFN-\gamma$ level on splenocyte culture significantly. but had no significant effects on expressions of ICAM-1, CD62L, integrin $a_4$. c-kit, IL-3 receptors. CD11a, or IgE receptors of BMMC. SM treatment significantly inhibited eosinophil infiltrates in BALf and peribronchial lung inflammation. Conculusions : The present data suggested that SM may have an effect on Th cytokine secretion and eosinophils associated with asthma responses. Therefore SM might be of therapeutic value in treating asthma.

키워드

참고문헌

  1. Bateman ED, Hurd SS, Barnes PJ, Bousquet J, Drazen JM, FitzGerald M etal. Global strategy for asthma management and prevention:GINA executive summary. Eur Respir J. 2008;31(1):143-78. https://doi.org/10.1183/09031936.00138707
  2. Okayama Y, Hagaman DD, Metcalfe DD. A comparison of mediators released or generated by IFN-gamma-treated human mast cells following aggregation of Fc gamma RI or Fc epsilon RI. J Immunol.2001;166(7):4705-12. https://doi.org/10.4049/jimmunol.166.7.4705
  3. Kidd P. Th1/Th2 balance:the hypothesis, its limitations, and implication for health and disease. Altern Med Rev.2003;8(3):223-46.
  4. 대한 천식 및 알레르기학회.천식과 알레르기질환.서울:군자출판사;2002,p.55-8,245-7.
  5. 채우석.면역질환의 한방개념과 치료에 관한 문헌적 고찰. 대한한의학회지.1990;11(2):54-91.
  6. 이권익,김성훈,성락기.단삼의 항암효과와 활성물질 분리에 관한 연구.대한동의병리학회지.1996;10(2):76-91.
  7. 윤현정,허숙경,윤형중,박원환,박선동. 단삼 메탄올 추출물의 항염증 효과. 대한본초학회지.2007;22(4):65-73.
  8. Kim SY, Moon TC, Chang HW, Son KH, Kang SS, Kim HP. Effects of tanshinone I isolated from Salvia miltiorrhiza bunge on arachidonic acid metabolism and in vivo inflammatory responses. Phytother Res. 2002; 16(7):616-20. https://doi.org/10.1002/ptr.941
  9. Murakami M, Austen KF, Arm JP. The immediate phase of c-kit ligand stimulation of mouse bone marrowderived mast cells elicits rapid leukotriene C4 generation through posttranslational activation of cytosolic phospholipase A2 and 5-lipoxygenase. J Exp Med.1995;182 :197-206. https://doi.org/10.1084/jem.182.1.197
  10. Mosmann TR, Cherwinski H, Bond MW, Giedlin MA, Coffman RL. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986;136(7) :2348-57.
  11. Seder RA, Paul WE. Acquisition of lymphokineproducting phenotype by CD4+ T cells. Annu RevImmunol.1994;12:635-73.
  12. Abbas AK, Lichtman AH, Pober JS. Cellular and Molecular Immunology. 4th ed. Philadelpia: W.B Saunders; 2000,p.260-1.
  13. Powrie F, Coffman RL. Cytokineregulation of T-cell function: potential for therapeutic intervention. Immunol Today.1993;14(6):270-4. https://doi.org/10.1016/0167-5699(93)90044-L
  14. 신민교.임상본초학.서울: 영림사; 2000, p.519-21.
  15. 양선아,임남경,이인선.단삼 메탄올 추출물의 항혈전 및 항산화 효과. 한국식품과학회지. 2007;39(1):83-7.
  16. Li D, Xiong SD, Du DB. Inhibitory effects of Salvia miltiorrhiza injection coordinated with dexamethasone on interleukin-13 and eotaxin expression in lung of asthmatic rats. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2006;26(11):1007-10.
  17. Wei ZM. Effecs of injection Salviae miltiorrhizae on senile chronic asthmatic bronchiti spatients. Zhongguo Zhong Xi Yi Jie He Za Zhi.1996;16(7):402-4.
  18. 張文江, 苗青, 張燕萍. 從瘀血論治哮喘的研究近况. 北京中醫藥大學學报.2004;11(3):53-4.
  19. 王炳文, 杨瑞玲, 王秀献. 活血化瘀爲主治療顽固性哮喘52例. 國醫論坛.1995;10(5):38.
  20. Levi-Schaffer F, Weg VB. Mast cells,eosinophils and fibrosis. Clin Exp Allergy. 1997;27(suppl) : 64-70. https://doi.org/10.1111/j.1365-2222.1997.tb01829.x
  21. Tedder TF, Steeber DA, Chen A, Engel P. The selectins: vascular adhesion molecules. FASEB J.1995;9(10):866-73 https://doi.org/10.1096/fasebj.9.10.7542213
  22. Toru H, Kinashi T, Ra C, Nonoyama S, Yata J, Nakahata T. Interleukin-4 induces homotypic aggregation of human mast cells by promoting LFA-1/ICAM-1 adhesion molecules. Blood. 1997; 89(9): 3296-302.
  23. Gauvreau GM, Becker AB, Boulet LP, Chakir J, Fick RB, Greene WL etal.The effects of ananti-CD11a mAb, efalizumab, on allergen induced airway responses and airway inflammation in subjects with atopic asthma. J Allergy Clin Immunol.2003;112(2):331-8. https://doi.org/10.1067/mai.2003.1689
  24. Laberge S, Rabb H,Issekutz TB, Martin JG. Roleof VLA-4 and LFA-1 inallergen-induced airway hyperresponsiveness and lung inflammation in the rat. Am J Respir Crit Care Med.1995;151(3Pt1):822-9. https://doi.org/10.1164/ajrccm/151.3_Pt_1.822
  25. Bhattacharyya SP, Drucker I, Reshef T, Kirshenbaum AS, Metcalfe DD, Mekori YA. Activated T lymphocytes induce degranulation and cytokine production by human mast cells following cell-to-cell contact. J Leukoc Biol.1998;63(3):337-41. https://doi.org/10.1002/jlb.63.3.337
  26. Xu J, Grewal IS, Geba GP, Flavell RA. Impaired primary T cell responses in L-selectin-deficient mice. J Exp Med. 1996;183(2):589-98. https://doi.org/10.1084/jem.183.2.589
  27. Rose DM, Han J, Ginsberg MH. Alpha4 integrins and the immunere sponse. Immunol Rev. 2002;186:118-24. https://doi.org/10.1034/j.1600-065X.2002.18611.x
  28. Abraham WM, Sielczak MW, Ahmed A, Cortes A, Lauredo IT, Kim J etal. Alpha 4-integrins mediate antigen-induced late bronchial responses and prolonged airway hyperresponsiveness in sheep. J Clin Invest.1994;93(2):776-87. https://doi.org/10.1172/JCI117032
  29. Al-Muhsen SZ, Shablovsky G, Olivenstein R, Mazer B, Hamid Q.Theexpression of stem cell factor and c-kit receptor in human asthmatic airways. Clin Exp Allergy. 2004;34(6):911-6. https://doi.org/10.1111/j.1365-2222.2004.01975.x
  30. Martinez-Moczygemba M,Huston DP.Biology of common beta receptor-signaling cytokines: IL-3, IL-5, and GM-CSF. J Allergy Clin Immunol. 2003;112(4):653-65 https://doi.org/10.1016/j.jaci.2003.08.015
  31. Gebhardt T, Sellge G, Lorentz A, Raab R, Manns MP, Bischoff SC.Cultured human intestinal mast cells express functional IL-3 receptorsand respond to IL-3by enhancing growth and IgE receptor-dependent mediator release. Eur J Immunol.2002;32(8):2308-16. https://doi.org/10.1002/1521-4141(200208)32:8<2308::AID-IMMU2308>3.0.CO;2-X
  32. Pawankar R. Mast cells as orchestrators of the allergic reaction: the IgE-IgE receptor mast cell network. Curr Opin Allergy ClinImmunol. 2001;1(1):3-6.
  33. Maurer D, Ebner C, Reininger B, Fiebiger E, Kraft D, Kinet JP et al.Thehigh affinity IgE receptor (Fc epsilon RI) mediates IgE dependent allergen presentation. J Immunol. 1995;154(12):6285-90.
  34. 주창엽,정희재,정승기,이형구.杏仁이 천식관련 chemokine분비와 호산구 chemotaxis에 미치는 영향. 대한한방내과학회지.2006:27(1):208-20.
  35. 정광진,정희재,정승기,이형구 금은화가 천식유발 cytokine분비와 호산구 chemotaxis에 미치는 영향. 대한한방내과학회지. 2005;26(1):129-42.
  36. 정승연,이성헌,황준호,이건영,김진주,정희재 등. 加味淸上補下湯 열수 및 에탄올 추출물이 천식 관련 cytokine 분비에 미치는 영향. 대한 한방내과학회지. 2006;27(2)521-32.