Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Effects of Momordica Charantia L. Water Extracts on the Rat Liver and Kidney with Acute Toxicated by Lead

여주 추출물이 급성 납 중독된 흰쥐의 간과 콩팥에 미치는 효과

  • Lee, Hyun-Hwa (Deptartment of Biology, College of Natural Science, Chosun University) ;
  • Cheong, Min-Ju (Deptartment of Biology, College of Natural Science, Chosun University) ;
  • Huh, Jin (Deptartment of Biology, College of Natural Science, Chonnam University) ;
  • Song, Seon-Young (Deptartment of Skin and Beauty, Gwangju Health College) ;
  • Boo, Hee-Ock (Deptartment of Biology, College of Natural Science, Chosun University)
  • 이현화 (조선대학교 자연과학대학 생물학과) ;
  • 정민주 (조선대학교 자연과학대학 생물학과) ;
  • 허진 (전남대학교 자연과학대학 생물학과) ;
  • 송선영 (광주보건대학 피부미용과) ;
  • 부희옥 (조선대학교 자연과학대학 생물학과)
  • Received : 2009.12.01
  • Accepted : 2009.12.28
  • Published : 2009.12.31
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Abstract

The purpose of this study was to demonstrate the effect of Momordica Charantia L. water extracts, one of the natural chelator, on the biochemical and enzyme activity changes in the rats liver and kidney caused by lead acetate. Rat approximately 250 g in weight were grouped into the control, lead acetate treated, and the Momordica Charantia L. boiling water extracts treated after lead acetate groups. Lead acetate (1,000 ppm) and Momordica Charantia L. water extracts (5%, 10%) were delivered drinking water. Serum AST, ALT and BUN were measured, histological alteration of liver and kidney were examined by light microscopy. Momordica Charantia L. extract group was decreased serum AST, ALT and BUN level induced by lead. Optical observations of liver tissue, lead group were observed necrosis of hepatic cells and infiltration of inflammatory cells, but Momordica Charantia L. extract group was observed only slight infiltration of inflammatory cells around the central vein. Optical observations of kidney tissue, lead acetate induced atrophy and necrosis of glomerulus and infiltration of inflammatory cell around renal tubule. For the group treated with Momordica charantia L. extract, the glomerulus was similar to the control, some around the renal tubule was observed infiltration of inflammatory cells. In conclusion, Momordica Charantia L. water extract may protect the lead-induced toxicity on liver and kidney.

본 연구는 급성 납 독성에 대한 여주 추출물의 효과를 알아보기 위하여 시도되었다. 250 g 내외의 흰쥐를 대상으로 대조군, 납(1,000 ppm) 투여군, 납 투여 후 필리핀산과 일본산 여주 추출물을 5%와 10%로 희석하여 식수로 공급한 후 6주 후에 간과 콩팥 손상 억제 효과를 알아보기 위하여 생화학적 및 조직학적 실험을 실시하였다. 혈액중 AST, ALT와 BUN의 수치는 여주 추출물 투여군이 납 단독투여군보다 감소되었다. 간 조직의 광학현미경적 관찰에서 납 단독투여군은 간세포의 괴사와 염증세포의 침윤이 관찰되었으나 여주 추출물 투여군에서는 중심정맥 주위에 약간의 단핵세포의 조직 침윤만이 관찰되었다. 콩팥 조직의 광학현미경적 관찰에서 납 단독투여군은 콩팥토리 모세혈관의 위축과 괴사 현상이 관찰되었고 콩팥세관 주위 간질에 염증세포의 침윤이 관찰되었으나 여주 추출물 투여군에서는 일부 콩팥세관 주위에서 염증 세포의 침윤만이 관찰되었다. 이상의 연구 결과로 보아 여주가 납으로 유발된 간과 콩팥 손상을 보호하는 효과가 있는 것으로 사료되며 여주에 대한 다양한 연구가 필요할 것으로 사료된다.

Keywords

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