Applied Microscopy

  • 제39권4호
  • /
  • Pages.325-332
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  • 2009
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
권호 표지

자몽추출물의 콩팥조직 부패억제 효과에 관한 현미경적 연구

Microscopic Study of Decomposition-Inhibition Effect in Grapefruit-seed Extract in Rat Kidney

  • 황규성 (을지대학교 보건산업대학 장례지도학과) ;
  • 최기주 (을지대학교 전자현미경실) ;
  • 남용석 (가톨릭대학교 의과대학 응용해부학연구소) ;
  • 임도선 (을지대학교 보건과학대학 치위생학과)
  • Hwang, Kyu-Sung (Department of Funeral Science, College of Health Industry, Eulji University) ;
  • Choi, Ki-Ju (Electron Microscopy Laboratory, Eulji University) ;
  • Nam, Yong-Seok (Department of Anatomy Catholic Institute for Applied Anatomy, College of Medicine, Catholic University) ;
  • Lim, Do-Seon (Department of Dental Hygiene, College of Health Science, Eulji University)
  • 투고 : 2009.11.10
  • 심사 : 2009.12.24
  • 발행 : 2009.12.31
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초록

광범위한 천연 소독제로서 사용되어온 자몽추출물의 조직에 대한 부패억제 효과를 확인하고자 본 연구를 수행하였다. 실험을 위해 8주령 SD계 흰쥐 콩팥(kidney)을 사용하였고, 자몽추출물을 처리하지 않은 대조군과, 55% 자몽추출물의 분말과 수용액을 처리한 실험군으로 구분하여 광학 및 전자현미경으로 관찰하였다. 광학현미경적 소견에서 대조군의 경우, 1일군부터 보우만주머니의 경계가 불분명해지기 시작하였고, 사구체 및 세뇨관의 형태로 부분적으로 괴사가 진행되었으며, 3일군에서 보우만주머니, 사구체 및 세뇨관의 형태가 대부분의 괴사나 유실되어 형태의 구분이 어려웠다. 그러나 실험군의 경우, 3일군부터 보우만주머니, 사구체 및 세뇨관 형태의 명확한 구별이 어려웠고, 부분적인 괴사가 관찰되었다. 한편, 전자현미경적 소견에서 대조군의 경우, 1일군은 바닥판의 일부가 붕괴되었으나 전체적인 형태는 유지되었고, 세포질 내 세포소기관의 붕괴로 전자밀도가 높은 응집된 형태로 관찰되었다. 2일군에서는 대부분이 붕괴되어 형체를 구별할 수 없었다. 그러나 실험군의 경우는 2일군에서 세포막의 부분적인 파괴가 확인되었고, 3일군에서 바닥판의 일부가 붕괴되기 시작하였고 세포소기관의 붕괴로 인한 응집 현상이 관찰되었다. 본 연구결과는 $37^{\circ}C$, 습도 $80{\pm}5%$에서 55% 자몽추출물은 부패와 변성을 억제하는 부패억제제로서 효과가 있고, 실험군의 3일군 조직의 형태가 대조군의 1일군 조직의 형태적 특성과 유사하게 나타났다. 따라서 자몽추출물은 최소 2일 이상의 부패억제 효과가 있는 것으로 확인되었다.

It has been well known that grapefruit seed extract (GSE) have effects on the deodorization and a wide range disinfectant. But anybody do not research about the decomposition-inhibition effect of GSE. The present study was performed to investigate the decomposition-inhibition effect of GSE by measuring the microscope and electroscope observation with passage of time. A total of 30 healthy Sprague-Dawley (SD) rats weighting from 230 gm to 250 gm were used as experimental animals. One group that do not treated by GSE named control group and the other group that treated by 55% GSE named Experimental group. Under ether anesthesia, right kidney was obtained. Put each sample in $37^{\circ}C$ and humidity $80{\pm}5%$ incubator, we obtained each sample after 0 day, 1 day, 2 days, 3 days, 4 days and 5 days. 4 ${\mu}m$ of paraffin sections were obtained, stained H-E stain and observed by use of a microscope. Morphological change in tissue was similar to control 1 day group and experimental 3 days group. Therefore, decomposition-inhibition effect of GSE continued about 2 days and it protected necrosis. According to above results, the author suggest that 55% GSE is an effective decomposition inhibitor until 2 days on $37^{\circ}C$ and humidity $80{\pm}5%$ conditions.

키워드

참고문헌

  1. Bea EA, Han MJ, Kim DH: In vitro anti-Helicobacter pyloriactivity of some flavonoids and their metabolites. Planta Med 65 : 442-443, 1999 https://doi.org/10.1055/s-2006-960805
  2. Blankson H, Grotterod EM, Seglen PO: Prevention of toxin-induced cytoskeletal disruption and apoptotic liver cell death by the grapefruit flavonoid, naringin. Cell Death Differ 7 : 739-746, 2000 https://doi.org/10.1038/sj.cdd.4400705
  3. Brzozowski T, Konturek PC, Drozdowicz D, Konturek SJ, Zayachivska Oxana, Pajdo R, Kwiecien S, Pawlik WW, Hahn EG: Grapefruit-seed extract attenuates ethanol-and stress-induced gastric lesion via activation of prostaglandin, nitric oxide and sensory nerve pathways. World J Gastroenterol 11(41) : 6450-6458, 2005 https://doi.org/10.3748/wjg.v11.i41.6450
  4. Cho SH, Lee SY, Kim JW, Ko GH, Seo IW: Development and application of natural antimicrobial agent isolated from grapefruit seed extract. J Fd Hyg Safety 10(1) : 33-39, 1995
  5. Cvetnic Z, Vladimir-Knezevic S: Antimicrobial activity of grapefruit seed and pulp ethanolic extracts. Acta Pharm 54 : 243-250, 2004
  6. Dorn JM, Hopkins BM: Thanatochemisty. Prentice-Hall, Inc., pp. 311-322, 1998
  7. Edwards-Jones V, Buck R, Sahwcross SG, Dawson MM, Dunn K: The effect of essential oils on methicillin-resistant S. aureus using a dressing model. Burns 30 : 772-777, 2004 https://doi.org/10.1016/j.burns.2004.06.006
  8. Heggers JP, Cottingham J, Gusman J, Reagor L, Mccoy L, Carino E, Cox R, Zhao JG: The effectiveness of processed grapefruit-seed extract as an antibacterial agent II. Mechanism of action and in vitro toxicity. J Altern Complement Med 8 : 333-340, 2002 https://doi.org/10.1089/10755530260128023
  9. Hwang KS, Choi KJ, Paik DJ, Lim DS: Microscopic study of decomposition- inhibition in stabilized ClO$_2$ gas in kidney of rat with passage of time. Korean J of Microscopy 38(3) : 259-264, 2008
  10. Keevil JG, Osman HE, Reed JD, Folts JD: Grape juice, but not orange juice or grapefruit juice, inhibits human platelet aggregation. J Nutr 130 : 53-56, 2000 https://doi.org/10.1093/jn/130.1.53
  11. Kim MK, Park MS, Choi SU, Park HR, Hwang YI: Antimicrobial effect of grapefruit seed xtracts on preservation for carrot and spinach. Journal of Life Science 15(1) : 66-70, 2005 https://doi.org/10.5352/JLS.2005.15.1.066
  12. Park HK, Kim SB: Antimicrobial activity of grapefruit seed extracts. Korean J Food & Nutr 19(4) : 526-531, 2006
  13. Reagor L, Gusman J, McCoy L, Carino E, Heggers JP: The effectiveness of related grapefruit-seed extract as an antibacterial agent I. An in vitro agar assay. J Altern Complement Med 8 : 325-332, 2002 https://doi.org/10.1089/10755530260128014
  14. Shoko T, Soichi T, Megumi MM, Eri F, Jun K, Michiko W: Isolation and identification of an antibacterial compound from grape and its application to food. Nippon Nogeikagaku Kaishi 73 :125-128, 1999 https://doi.org/10.1271/nogeikagaku1924.73.125
  15. So FV, Guthrie N, Chambers AF, Moussa M, Carroll KK: Inhibition of human breast cancer cell proliferation and delay of mammary tumorigenesis by flavonoids and citrus juices. Nutrcancer 26 :167-181, 1996 https://doi.org/10.1080/01635589609514473
  16. Sundberg C, J$\ddot{o}$nsson H: Higher pH and faster decomposition in biowaste composting by increased aeration. Waste Management 28 : 518-526, 2008 https://doi.org/10.1016/j.wasman.2007.01.011
  17. Sundheim G, Langsrud S: Natural and acquired resistance of bacteria associated with food processing environments to disinfectant containing an extract form grapefruit seeds. International Biodeterioration & Biodegradation : 441-448, 1995
  18. Tirillini B: Grapefruit. the last decade acquisitions. Fitoterapia 71 : 29-37, 2000 https://doi.org/10.1016/S0367-326X(00)00176-3
  19. von Woedtke T, Schl$\ddot{u}$ter B, Pflegel P, Lindequist U, J$\ddot{u}$lich WD: Aspects of the antimicrobial efficacy of grapefruit seed extract and its relation to preservative substances contained. Pharmazie 54(6) : 452-456, 1999
  20. Xu W, Qu W, Huang K, Guo Feng, Yang J, Zhao H, Luo Y: Antibacterial effect of grapefruit seed extract on food-borne pathogens and its application in the preservation of minimally processed vegetables. Postharvest Biology and Technology 45 : 126-133, 2007 https://doi.org/10.1016/j.postharvbio.2006.11.019
  21. Zayachkivska OS, Konturek SJ, Drozdowich D, Konturek PC, Brzozowski T, Ghegotsky MR: Gastroprotective effects of flavenoids in plant extracts. J Physiol Pharmacol 56 : 219-231, 2005