Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Expression of Decidual Natural Killer (NK) Cells in Recurrent Abortion Patients with Increased Peripheral NK Cells

말초혈액 자연살해세포가 증가된 반복유산 환자의 탈락막 자연살해세포의 발현

  • Lee, Hyun-Joo (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center) ;
  • Kim, Sun-Haeng (Department of Obstetrics and Gynecology School of Medicine, Korea University) ;
  • Yang, Kwang-Moon (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center) ;
  • Park, Dong-Wook (Laboratory of Reproductive Biology & Infertility, Korea University) ;
  • Park, Chan-Woo (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center) ;
  • Hong, Sung-Ran (Department of Pathology, Korea University) ;
  • Cho, Dong-Hee (Department of Clinical Pathology, Korea University) ;
  • Song, In-Ok (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center) ;
  • Koong, Mi-Kyoung (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center) ;
  • Kang, Inn-Soo (Department of Obstetrics & Gynecology, Cheil General Hospital & Women's Healthcare Center)
  • 이현주 (관동대학 의과대학 제일병원 산부인과) ;
  • 김선행 (고려대학교 의과대학 산부인과학교실) ;
  • 양광문 (관동대학 의과대학 제일병원 산부인과) ;
  • 박동욱 (고려대학교 생식생물학물 불임연구실) ;
  • 박찬우 (관동대학 의과대학 제일병원 산부인과) ;
  • 홍성란 (고려대학교 진단병리과) ;
  • 조동희 (고려대학교 임상병리과) ;
  • 송인옥 (관동대학 의과대학 제일병원 산부인과) ;
  • 궁미경 (관동대학 의과대학 제일병원 산부인과) ;
  • 강인수 (관동대학 의과대학 제일병원 산부인과)
  • Published : 2009.09.30
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Abstract

Objectives: The purpose of this study was to compare the decidual NK cell populations between increased pre-conceptional peripheral NK cell population and normal pre-conceptional peripheral NK cell population in women with a history of recurrent abortion. Methods: Fourteen women with history of recurrent abortion and elevated pre-conceptional peripheral NK cell, above 15% of peripheral lymphocyte population were included in this study. As a control, twelve women with history of recurrent abortion and their peripheral NK cell percentage showed below 15% were included. Distribution of $CD56^+$ and $CD16^+$ NK cells in paraffin embedded decidual tissues including implantation sites were examined by immunohistochemical staining using anti-CD56, 16 monoclonal antibodies. After immuohistochemical staining, the numbers of decidual NK cells were counted and compared these results between study and control groups. Results: There was significant difference in decidual $CD56^+$ NK cell count ($170.1{\pm}132.1$ vs. $68.3{\pm}66.1$, p=0.02) between increased peripheral $CD56^+$ NK cell group and control group. But, there showed no statistically significant correlation between decidual $CD56^+$ NK cell count and peripheral $CD56^+$ NK cell percentage (r=0.229, p=0.261). Also there was no statistically difference decidual $CD16^+$ NK cell count between study and control group ($25.70{\pm}11.72$ vs. $31.17{\pm}22.67$), and no correlation between decidual $CD16^+$ NK cell and peripheral $CD16^+$ NK cell percentage (r=-1.40, p=0.535). Conclusions: This study shows that decidual $CD56^+$ NK cell are significantly increased in decidua of women exhibiting a history of recurrent abortion with increased $CD56^+$ peripheral NK cell. This study suggests that the percentage of peripheral NK cell reflect the expression of decidual NK cell. Consequently, pre-conceptional peripheral blood NK cell population can be the useful marker for detecting the risk of subsequent miscarriage.

목 적: 말초혈액 자연살해세포가 증가된 반복유산 환자군과 증가되지 않은 반복유산 환자군에서 다음 번 유산 시탈락막의 자연살해세포의 발현양상을 관찰하여 말초혈액 자연살해세포와 상관관계가 있는지 보고자 하였다. 연구방법: 말초혈액 자연살해세포가 15% 이상 증가된 반복유산 환자군 14명을 실험군으로, 15% 이하인 군 12명을 대조군으로 하여 연구를 진행하였다. 이 환자들에게서 다음 번 유산 시 착상부위를 포함한 자궁 탈락막 세포를 확인하여 면역조직화학염색을 통해 $CD56^+$와 $CD16^+$ 자연살해세포의 수를 세어 실험군과 대조군을 비교하였다. 결 과: 실험군과 대조군은 탈락막 내 $CD56^+$ 자연살해세포의 수 간에 통계적으로 유의한 차이 ($170.1{\pm}132.1$ vs. $68.3{\pm}66.1$, p=0.02)를 보였으나 둘 간의 상관관계 (r=0.229, p=0.261)는 없었다. 또한 두 군간에 $CD16^+$ 자연살해세포는 통계적으로 유의한 차이 ($25.70{\pm}11.72$ vs. $31.17{\pm}22.67$)를 보이지 않았다. 결 론: 본 연구는 말초혈액 $CD56^+$ 자연살해세포가 증가된 반복유산 환자군에서 말초혈액 자연살해세포가 증가되지 않은 군에 비하여 탈락막의 $CD56^+$ 자연살해세포가 증가되어 있음을 알 수 있었다. 이는 탈락막 자연살해세포가 반복유산의 면역학적 기전에서 중요한 역할을 할 것이라는 가능성을 제시해 주는 연구 결과이며 또한 말초혈액 자연살해세포의 측정이 반복유산 환자에서 다음 번 유산을 예측할 수 있는 유용한 지표임을 알 수 있다.

Keywords

References

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