Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characteristics of B-Cell-Specific Growth Substance Produced by Bacillus licheniformis E1

  • Kim, Joo-Young (Department of Internal Medicine, Yonsei University College of Medicine) ;
  • Chung, Kun-Sub (Department of Biological Resources and Technology, Yonsei University) ;
  • Park, Jeon-Han (Department of Microbiology, Yonsei University College of Medicine) ;
  • Kwak, Yi-Sub (Department of Physical Education, Dong-Eui University) ;
  • Lee, Bong-Ki (Department of Microbiology, Yonsei University College of Medicine)
  • Received : 2008.06.26
  • Accepted : 2008.09.03
  • Published : 2009.01.31
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Abstract

A B cell-specific growth substance (BGS) was isolated from the slime layer of Bacillus licheniformis E1. Unlike LPS, the BGS was not affected by polymixin B, an inhibitor of LPS, or by TLR4, and resulted in the growth of B cells. When BALB/c mice were treated with the BGS, the B cell population was found to increase in both the bone marrow and the spleen, with a marked increase after 24 h in the bone marrow and after 48 h in the spleen. When using antibodies to B cell lineage-restricted surface molecules to analyze the B cell population changes resulting from treatment with the BGS, an increase in immature B cells ($IgM^+$ and $AA4.1^+$) and mature B cells ($IgM^+$ and $IgD^+$) was found in the bone marrow 24 h after treatment with the BGS, whereas a decrease in mature B cells and increase in $IgG^+$ B cells were found in the spleen. When the BGS and OVA antigen were injected into the peritoneal cavity of BALB/c mice, this resulted in a high OVA-specific antibody titer in the sera, similar to that induced by aluminum hydroxide. Therefore, it is anticipated that the mass production of the BGS by B. licheniformis E1 could be used for studies of B cells in immunology, and contribute to the development of a new adjuvant for vaccine manufacture.

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References

  1. Abbas, A. K., A. H. Lichtman, and S. Pillai. 2007. Cellular and Molecular Immunology. Saunders Elsevier, Philadelphia, PA, U.S.A
  2. Ahmed, R. and D. Gray. 1996. Immunological memory and protective immunity: Understanding their relation. Science 272: 54-60 https://doi.org/10.1126/science.272.5258.54
  3. Allman, D. M., S. E. Ferguson, and M. P. Cancro. 1992. Peripheral B cell maturation. I. Immature peripheral B cells in adults are heat-stable $antigen^{hi}$ and exhibit unique signaling characteristics. J. Immunol. 149: 2533-2540
  4. Allman, D. M., S. E. Ferguson, V. M. Lentz, and M. P. Cancro. 1993. Peripheral B cell maturation. II. Heat-stable antigen (hi) splenic B cells are an immature developmental intermediate in the production of long-lived marrow-derived B cells. J. Immunol. 151: 4431-4444
  5. Borsutzky, S., K. Kretschmer, P. D. Becker, P. F. Muhlradt, C. J. Kirschning, S. Weiss, and C. A. Guzman. 2005. The mucosal adjuvant macrophage-activating lipopeptide-2 directly stimulates B lymphocytes via the TLR2 without the need of accessory cells. J. Immunol. 174: 6308-6313 https://doi.org/10.4049/jimmunol.174.10.6308
  6. Brack, C., M. Hirama, R. Lenhard-Schuller, and S. Tonegawa. 1978. A complete immunoglobulin gene is created by somatic recombination. Cell 15: 1-14 https://doi.org/10.1016/0092-8674(78)90078-8
  7. Brightbill, H. D., D. H. Libraty, S. R. Krutzik, R. B. Yang, J. T. Belisle, J. R. Bleharski, et al. 1999. Host defense mechanisms triggered by microbial lipoproteins through toll-like receptors. Science 285: 732-736 https://doi.org/10.1126/science.285.5428.732
  8. Chung, K. S., J. Y. Kim, S. W. Hong, and B. K. Lee. 2006. Isolation of bacteria producing a B-cell-specific biological response modifier found in Korean fermented soybean paste. J. Microbiol. Biotechnol. 16: 126-135
  9. Forster, I. and K. Rajewsky. 1990. The bulk of the peripheral Bcell pool in mice is stable and not rapidly renewed from the bone marrow. Proc. Natl. Acad. Sci. USA 87: 4781-4784 https://doi.org/10.1073/pnas.87.12.4781
  10. Gray, D. 1993. Immunological memory. Annu. Rev. Immunol. 11: 49-77 https://doi.org/10.1146/annurev.iy.11.040193.000405
  11. Guy, B. 2007. The perfect mix: Recent progress in adjuvant research. Nat. Rev. Microbiol. 5: 505-517 https://doi.org/10.1038/nrmicro1681
  12. Han, S. B., Y. D. Yoon, H. J. Ahn, H. S. Lee, C. W. Lee, W. K. Yoon, S. K. Park, and H. M. Kim. 2003. Toll-like receptormediated activation of B cells and macrophages by polysaccharide isolated from cell culture of Acanthopanax senticosus. Int. Immunopharmacol. 3: 1301-1312 https://doi.org/10.1016/S1567-5769(03)00118-8
  13. Hayashi, E. A., S. Akira, and A. Nobrega. 2005. Role of TLR in B cell development: Signaling through TLR4 promotes B cell maturation and is inhibited by TLR2. J. Immunol. 174: 6639-6647 https://doi.org/10.4049/jimmunol.174.11.6639
  14. Hem, S. L. and H. Hogenesch. 2007. Relationship between physical and chemical properties of aluminum-containing adjuvants and immunopotentiation. Expert Rev. Vaccines 6: 685-698 https://doi.org/10.1586/14760584.6.5.685
  15. Hu, H. and G. Moller. 1994. Lipopolysaccharide-stimulated events in B cell activation. Scand. J. Immunol. 40: 221-227 https://doi.org/10.1111/j.1365-3083.1994.tb03454.x
  16. Imler, J. L. and J. A. Hoffmann. 2001. Toll receptors in innate immunity. Trends Cell Biol. 11: 304-311 https://doi.org/10.1016/S0962-8924(01)02004-9
  17. Jo, W. S., S. T. Yee, S. Yoon, B. H. Nam, E. Do, B. S. Jung, et al. 2006. Immunostimulating factor isolated from Actinobacillus actinomycetemcomitans stimulates monocytes and inflammatory macrophages. Microbiol. Immunol. 50: 535-542 https://doi.org/10.1111/j.1348-0421.2006.tb03823.x
  18. Lavelle, E. C., P. McGuirk, and K. H. Mills. 2004. Molecules of infectious agents as immunomodulatory drugs. Curr. Top. Med. Chem. 4: 499-508 https://doi.org/10.2174/1568026043451249
  19. Leenaars, P. P., M. A. Koedam, P. W. Wester, V. Baumans, E. Claassen, and C. F. Hendriksen. 1998. Assessment of side effects induced by injection of different adjuvant/antigen combinations in rabbits and mice. Lab. Anim. 32: 387-406 https://doi.org/10.1258/002367798780599884
  20. Lortan, J. E., C. A. Roobottom, S. Oldfield, and I. C. MacLennan. 1987. Newly produced virgin B cells migrate to secondary lymphoid organs but their capacity to enter follicles is restricted. Eur. J. Immunol. 17: 1311-1316 https://doi.org/10.1002/eji.1830170914
  21. Lu, M., M. Zhang, A. Takashima, J. Weiss, M. A. Apicella, X. H. Li, D. Yuan, and R. S. Munford. 2005. Lipopolysaccharide deacylation by an endogenous lipase controls innate antibody responses to Gram-negative bacteria. Nat. Immunol. 6: 989-994 https://doi.org/10.1038/ni1246
  22. Mamchak, A. A. and P. D. Hodgkin. 2000. Regulation of lipopolysaccharide-induced B-cell activation: Evidence that surface immunoglobulin mediates two independently regulated signals. Immunol. Cell. Biol. 78: 142-148 https://doi.org/10.1046/j.1440-1711.2000.00896.x
  23. Matsunaga, K., A. Hosokawa, M. Oohara, N. Sugita, M. Harada, and K. Nomoto. 1998. Direct action of a proteinbound polysaccharide, PSK, on transforming growth factor-beta. Immunopharmacology 40: 219-230 https://doi.org/10.1016/S0162-3109(98)00045-9
  24. Morrison, D. C. and D. M. Jacobs. 1976. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry 13: 813-818 https://doi.org/10.1016/0019-2791(76)90181-6
  25. Muller, A., P. J. Rice, H. E. Ensley, P. S. Coogan, J. H. Kalbfleish, J. L. Kelley, et al. 1996. Receptor binding and internalization of a water-soluble (1-->3)-beta-D-glucan biologic response modifier in two monocyte/macrophage cell lines. J. Immunol. 156: 3418-3425
  26. Obayashi, K., T. Doi, and S. Koyasu. 2007. Dendritic cells suppress IgE production in B cells. Int. Immunol. 19: 217-226 https://doi.org/10.1093/intimm/dxl138
  27. Rehli, M. 2002. Of mice and men: Species variations of tolllike receptor expression. Trends Immunol. 23: 375-378 https://doi.org/10.1016/S1471-4906(02)02259-7
  28. Reth, M. 1992. Antigen receptors on B lymphocytes. Annu. Rev. Immunol. 10: 97-121 https://doi.org/10.1146/annurev.iy.10.040192.000525
  29. Rolink, A. G., J. Andersson, and F. Melchers. 1998. Characterization of immature B cells by a novel monoclonal antibody, by turnover and by mitogen reactivity. Eur. J. Immunol. 28: 3738-3748 https://doi.org/10.1002/(SICI)1521-4141(199811)28:11<3738::AID-IMMU3738>3.0.CO;2-Q
  30. Siegelman, M. H., H. C. DeGrendele, and P. Estess. 1999. Activation and interaction of CD44 and hyaluronan in immunological systems. J. Leukoc. Biol. 66: 315-321 https://doi.org/10.1002/jlb.66.2.315
  31. Snapper, C. M., F. R. Rosas, L. Jin, C. Wortham, M. R. Kehry, and J. J. Mond. 1995. Bacterial lipoproteins may substitute for cytokines in the humoral immune response to T cellindependent type II antigens. J. Immunol. 155: 5582-5589
  32. Soares-Schanoski, A., V. Gomez-Pina, C. Del Fresno, A. Rodriguez-Rojas, F. Garcia, A. Glaria, et al. 2007. 6-Methylprednisolone down-regulates IRAK-M in human and murine osteoclasts and boosts bone-resorbing activity: A putative mechanism for corticoid-induced osteoporosis. J. Leukoc. Biol. 82: 700-709 https://doi.org/10.1189/jlb.1106673
  33. Tzianabos, A. O. 2000. Polysaccharide immunomodulators as therapeutic agents: Structural aspects and biologic function. Clin. Microbiol. Rev. 13: 523-533 https://doi.org/10.1128/CMR.13.4.523-533.2000
  34. Tzianabos, A. O., P. R. Russell, A. B. Onderdonk, F. C. Gibson 3rd, C. Cywes, M. Chan, R. W. Finberg, and D. L. Kasper. 1999. IL-2 mediates protection against abscess formation in an experimental model of sepsis. J. Immunol. 163: 893-897
  35. Wakshull, E., D. Brunke-Reese, J. Lindermuth, L. Fisette, R. S. Nathans, J. J. Crowley, et al. 1999. PGG-glucan, a soluble beta-(1,3)-glucan, enhances the oxidative burst response, microbicidal activity, and activates an NF-kappa B-like factor in human PMN: Evidence for a glycosphingolipid beta-(1,3)-glucan receptor. Immunopharmacology 41: 89-107 https://doi.org/10.1016/S0162-3109(98)00059-9
  36. Wang, Y., S. P. Li, S. A. Moser, K. L. Bost, and J. E. Domer. 1998. Cytokine involvement in immunomodulatory activity affected by Candida albicans mannan. Infect. Immun. 66:1384-1391